From Cell Cultures (~2 × 108 cells)
Perform the procedure at 4 °C. All solutions and
equipment should be cooled before use. Homogenize
the samples using a 7 mL Dounce glass tissue grinder
Dilution Buffer:
3.1. For tissue extracts from 4 g of tissue: 40 mL
3.2. For cell culture extracts from at least
2 × 108 cells: 30 mL
OptiPrep™ Density Gradient Medium Solutions
retrovirus)
Run 1: 3.3 x 108 Run 1: 1.5 x 103
5.4 ± 0.0
Run 2: 3.3 x 108 Run 2: 1.5 x 103
MVM (parvovirus)
Run 1: 1.4 x 108 Run 1: 5.5 x 105
2.3 ± 0.1
Run 2:
(1994)
14. Jendraschak, E. and E.H. Sage, Semin. Cancer
Biol., 7, 139 (1996)
15. Leddda, R. et al., J. Invest. Dermatol. 108, 210
(1997)
16. Strandjord, T.P. et al., Am. J. Respir. Cell Mol. Biol
challenge of 108–1010 plaque forming units of φX–174 virus
particles at 50 SCFM per 10-inch cartridge.
6
Air Flow Rate and Pressure Drop – Opticap® XL Capsule Filters
0 10 20
Geobacillus stearothermophilus spore suspension, adjusted to a con-
centration of 108 KBE/ml (stray range: 7 · 107 to 3 · 108 KBE/ml).
Test agar for the antibiotics residue test according to KUNDRAT,
Cat.
EA7S
(1.5 × 108 cells/ml)
(µl)
GVB2+
(µl)
Distilled
water
(µl)
A** v – 200 300–v –
B v 5 200 295–v –
C v 10 200 290–v –
D v 20 200 280–v –
E v 30 200 270–v
Erythrocytes
(1.5 × 108 cells/ml)
(µl)
GVB2+
(µl)
Distilled
water
(µl)
A** v – 200 300–v –
B v 5 200 295–v –
C v 10 200 290–v –
D v 20 200 280–v –
E v 30 200 270–v
Syringe Filter 7 min. 12 sec. n/a 4 min. 6 sec. 2 min. 5 min. 25 sec. 15 min. 40 sec.
Supplier Y Syringe Filter 2 min. 30 sec. 2 min. 3 sec. 4 min. 4 min. 30 sec.