vial, spin down the solid to the bottom by a microcentrifuge or by a desktop centrifuge.
2. Add 109 μl of high-purity DMSO to one vial of Dye to make a 1 mM stock solution.
3. Dilute the stock
,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼74 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 81–109 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
C (see
Appendix A, Recipes).
Chill 100% and 70% ethanol solutions at -20°C.
Equilibrate a heating block or water bath at 65°C
Sample Preparation:
From a 6- or 12-well microtiter plate choose an
occlusion-negative
Anti-Paip2 is produced in rabbit using as immunogen a
synthetic peptide corresponding to amino acids 93-109
of human Paip2 (GeneID: 51247), conjugated to KLH
via an added cysteine residue. The immunizing peptide
fluid by Protein A chromatography.
Monoclonal Anti-Prion Protein recognizes amino acid
residues 109-112 of PrP from human, hamster and
feline by immunoblotting, ELISA, immunoprecipitation
and immunohistochemistry
least 6 months at -20 °C. A stock solution containing at
least 20 µg protein per ml can be stored under sterile
conditions at 2-8 °C for one month and in single-use
aliquots for six months at −70 °C without
3rd ed. (Rose, N.R.
et al., eds.). American Society for Microbiology
(Washington, D.C.), pp. 99-109 (1986).
4. Mlinar, Diana, "The Role of Trappin-2 and RANTES
in Mediating Resistance to HIV-1 Infection
pp. 1552-1566 (1961).
5. Fariss, M.W., and Reed, D.J., Methods Enzymol.,
143, 101-109 (1987).
6. Lorusso, M. et al., FEBS Lett., 195(1-2), 298-302
(1986).
7. Pontremoli, S. et al., J
3rd ed. (Rose, N.R.
et al., eds.). American Society for Microbiology
(Washington, D.C.), pp. 99-109 (1986).
4. O'Reilly, Isobel, "Potentiation of Drug-Induced
Cytotoxicity by Conjugated Linoleic
Code PROT-RA). 109 µg of protein was loaded
on a 7 cm, pH 4-7 IPG strip (Product Code I 2906),
focused for 50,000 volt hours, then run on a 4-20%
Tris-Glycine SDS-PAGE gel at 150 volts for 70 min. The
gel
3rd ed. (Rose, N.R.
et al., eds.). American Society for Microbiology
(Washington, D.C.), pp. 99-109 (1986).
4. Rasmussen, Mads Skytte, "Characterization of
Matrix Derived Effects on Osteoclast
(Washington, D.C.), pp. 99-109 (1986).
5. Bauerle, W. L. et al., J. Amer. Soc. Hort. Sci.,
131(2), 295-301 (2006).
6. Dahabreh, Z. et al., World J. Stem Cells, 6(4),
497-504 (2014).
7. Ásgeirsson
5-8
Human
PDGF-BB is a 24.3 kDa B chain homodimer protein
containing 109 amino acid residues.
In platelets, 70% of the PDGF is present as the
AB dimer, with most of the remainder as
3rd ed. (Rose, N.R.
et al., eds.). American Society for Microbiology
(Washington, D.C.), pp. 99-109 (1986).
4. Hsu, Ching-Yin, "Application of Silver
Nanoparticle-based Materials in Orthopedic
. Phase contrast or light microscope
4. Polypropylene tubes
5. Cells / Tissue samples
6. 70% glycerol (optional)
Precautions
• Please refer to the Material Safety Data Sheet at www.chemicon.com
Gelatin as a delivery vehicle for
the controlled release of bioactive molecules. J.
Control Release, 109(1-3), 256-274 (2005).
9. Huang Y, et al., In vitro characterization of
chitosan-gelatin scaffolds
: Methods and Applications. Kruse, P. and Patterson, M. (eds). New
York Academic Press, pp 106-109.
10. Yamada, K. and Akiyama, S. (1984). Preparation of Cellular Fibronectin. In: Methods for Preparation
: Methods and Applications. Kruse, P. and Patterson, M. (eds). New
York Academic Press, pp 106-109.
10. Yamada, K. and Akiyama, S. (1984). Preparation of Cellular Fibronectin. In: Methods for Preparation