substantially
more expressing clones than non-UCOE-derived
vectors
• Cell lines stable over 130 generations
• High-yielding cell lines can be derived in
less than 60 days, without amplification
the purification performance.
20442226w Rev 06/20 3 of 4
Note: For some tissue types, lysis is already
complete after 15 minutes. Therefore, this step
may be shortened accordingly.
Meanwhile
the purification performance.
20442226w Rev 06/20 3 of 4
Note: For some tissue types, lysis is already
complete after 15 minutes. Therefore, this step
may be shortened accordingly.
Meanwhile
AA, 175% Metals, 70% Iron 1
50% AA, 25% Metals, 130% Iron 1 150% AA, 25% Metals, 130% Iron 1
50% AA, 175% Metals, 130% Iron 1 150% AA, 175% Metals, 130% Iron 1
Centerpoint Base Medium
A
B C
20 v/v)
Detection: LC-MS, positive ESI mode, SIM; m/z 130
Flow Rate: 0.5 mL/min
Mobile Phase: Acetonitrile and 10 mM ammonium acetate, pH 6.8 (85:15 v/v). Total ionic strength: 1.5 mM
Temperature: 30 °C
immunoblotting (130 kDa)
and immunoprecipitation. Staining of p130CAS in
immunoblotting is specifically inhibited with the
immunizing peptide.
p130CAS (Crk-associated protein, Cas) is a 120-130 kDa
adaptor
Loading.
Sample Loading
2. Add an equal volume (either 300 μL or 130 μL) of
RNA Wash Buffer 3 and pipet up and down at least
10-15 times.
3. Place RNA Binding Plate on
SPKMBSMTTANK004 SMART TFF, Tank Cart, Pump Cart Hook Kit X Other No As required
#130 SPKMBSMTTANK005 SMART TFF, Tank Cart, Set of 4 Leveling Feet X Other No As required
#131 SPKMBSMTTANK006 SMART TFF, Tank
or packing slip.
4. Lu, R., and Serrero, G., Proc. Natl. Acad. Sci.
USA, 98, 142-147 (2001).
5. Ryan, C.L., et al., BMC Neurosci., 10, 130 (2009)
doi :10.1186/I47I-2202-10-130.
6. Baker, M., et
87909001
VS 130 x 500 x 500 87913001
VS 180 x 500 x 500 87918001
VS 250 x 500 x 500 87925001
Column Tube Extension Kit
VTKS 60 x 500 87906005
VTKS 90 x
, 593-599 (2003).
4. Lu, R., and Serrero, G., Proc. Natl. Acad. Sci. USA,
98, 142-147 (2001).
5. Ryan, C.L., et al., BMC Neurosci., 10, 130 (2009)
doi :10.1186/I47I-2202-10-130.
6. Baker, M., et
0.5 mL/min
Mobile Phase: 4 gm of ammonium acetate& 7.0 gm of sodium perchlorate in 1300 ml water.
Adjust pH to 2.2 with orthophosphoric acid. Mix Buffer & Acetonitrile 130:24 (v/v)
Temperature: 45 °
step add 300 μL or 130 μL RNA
Wash Buffer 3 (for lysates from cells grown in 96-
well plates), to each well of the lysis plate or tube.
Mix by pipetting up and down at least 10 - 15
times.
for nitric
oxide synthase (NOS), derived from activated mouse
macrophage RAW 264.7 cells (iNOS, 130 kDa), but
does not recognize NOS derived from brain tissue
(bNOS) and endothelial cells (eNOS). By
morphogenesis (embryonic axis formation) and
has been linked to some forms of cancer.1-4 Axin1 (axis
inhibitor-1, axin, 130 kDa) acts as a negative regulator
of Wnt signaling. It directly interacts with various
ion-exchange chromatography. Anti-
ASAP1/Centaurin β4 recognizes human ASAP1 by
immunoblotting (130 kDa, doublet band). Staining of
ASAP1 in immunoblotting is specifically inhibited with
the ASAP1
step add 300 L or 130 L of RNA
Wash Buffer 3 (for lysates from cells grown in
96 well plates), to each well of the lysis plate or
tube. Mix by pipetting up and down at least
10-15 times.