ion-exchange chromatography. Anti-
ASAP1/Centaurin β4 recognizes human ASAP1 by
immunoblotting (130 kDa, doublet band). Staining of
ASAP1 in immunoblotting is specifically inhibited with
the ASAP1
(VG-17) (Prod. No. A 4102)
MW
130 kDa
160
95
75
Mouse brain (Lane A, C) and rat brain (Lane B)
cytosolic fractions were separated on SDS-PAGE and
blotted with Rabbit Anti-ASAP1 (VG-17)
(Prod
1:2,000 - 1:500 dilution) by immunoblotting
using HeLa or Jurkat cell lysates. A wide band of 80-
130 kDa can be detected. This represents mature
protein, precursor and glycosylated TACE.
Note: In
Tissue
plasminogen activators: chemical and physiological
aspects. Semin. Thromb. Hemos., 10(1), 6-17
(1984).
RBG/JBB/MAM 10/02
Sigma brand products are sold through Sigma-Aldrich, Inc.
Anti-Axin1 (C-terminal region) specifically recognizes
human and mouse axin1 by immunoblotting (~130 kDa).
Staining of the axin1 band in immunoblotting is
specifically inhibited by the immunizing peptide
immunizing peptide immobilized on
agarose.
Anti-Axin1 recognizes mouse axin1 by immunoblotting
(~130 kDa). Staining of the axin1 band in
immunoblotting is specifically inhibited by the
immunizing peptide
course of activation (e.g. 349 ± 130 ms at -110
mV and 104 ± 32 ms at -140 mV, n=6). Under whole-cell recording conditions
the mean current amplitude was 2.7 ± 0.5 nA at -130 mV (n =10) and reversal
using BSA as a calibrator. The correction factor
from the Bradford method to Amino Acid Analysis is
130% for this protein.
Identity: Confirmed by peptide mapping
Purity: 95% (SDS-PAGE)
UniProt
-Arg
511
,
with a C-terminal 8-histidine tag). The DTT-reduced
protein migrates as a 100–130 kDa polypeptide on
SDS-PAGE because of glycosylation. This protein is
manufactured in human cells
muscle cells, and chondrocytes.7 The enzyme
is homodimer and each subunit has a molecular mass
of 130 kDa. It is also known as macrophage NOS
(mNOS) or inducible NOS (iNOS).
Type III NOS is found
agarose.
Anti-Axin1 (C-terminal) specifically recognizes rat and
mouse axin1 by immunoblotting (~130 kDa). Staining of
the axin1 band in immunoblotting is specifically
inhibited by the immunizing peptide
using BSA as a calibrator. The correction factor
from the Bradford method to Amino Acid Analysis is
130% for this protein.
Identity: Confirmed by peptide mapping
Purity: 95% (SDS-PAGE)
inducible (iNOS) by immunoblotting and
immunohistochemistry. In immunoblotting, the antibody
detects a 130 kDa band representing iNOS in samples
first induced with IFN (interferon) and LPS
(lipopolysaccharides
Kreutzberg, G., Neuroscience, 20,
23 (1987).
16. Godfrey, E., and Shooter, E., J. Neurosci., 6, 2543
(1986).
17. Marchetti, D., and Perez-Polo, J., J. Neurochem.,
49, 475 (1987).
18. Buxser, S., et
wash step with dig-wash buffer before
digestion.
Library
Preparation
A prominent peak
at ~130 bp is
detected by
Bioanalyzer
analysis in a large
number of
samples
This peak corresponds
immunoblotting (130 kDa)
and immunoprecipitation. Staining of p130CAS in
immunoblotting is specifically inhibited with the
immunizing peptide.
p130CAS (Crk-associated protein, Cas) is a 120-130 kDa
adaptor