LOK (1-348) Active (L3794) - Datasheet
LOK (1-348) Active
human, recombinant
GST-tagged, expressed in Sf9 cells
Catalog Number L3794
Lot Number 019K1618
Storage Temperature –70 °C
Synonyms
LOK (1-348) Active (L3794) - Datasheet
LOK (1-348), active, GST-tagged, human
Precisio Kinase
recombinant, expressed in Sf9 cells
Catalog Number L3794
Lot Number 060M0858
Storage Temperature
T., et al., J. Immunol., 105, 1439 (1970)
5. Martin, A., et al., Immunochemistry, 13, 317-324
(1976)
6. Thomas, A.E., et al., J. Immunology, 113, 1, 348-
358 (1974)
7. Curman, B. et al., Biochemistry
potassium
phosphate, 330 mM NaCl,
1 mM EDTA,
5 mM dithioerythritol,
0.02% polydocanol (v/v),
50% glycerol (v/v),
pH approximately 7.0 (+4°C).
10 656 348 001 1 vial,
5,000 U (10 U/μl)
Sma I
Immunogen: Recombinant protein E2F transcription factor
2
Immunogen Sequence: GI # 4758226, sequence 348 - 437
The product is a clear, colorless solution in phosphate
buffered saline, pH 7.2, containing
the nitrocellulose twice with water.
5. Incubate the nitrocellulose in the secondary reagent of choice (a goat anti-rabbit HRP conjugated IgG, Catalog #
12-348, 1:5000 dilution was used) in PBS-MLK for
compression) with Eshmuno
®
S before (blue) and after storage in
1M NaOH (red, dashed line) for 348 days.
BDM SO3 DMAA 08Re52 08 100 16 col3004:10_UV1_280nm BDM SO3
Sakurai, T. et al., Nature, 348, 732 (1990).
3. Cheng, H.F. et al., Mol. Pharmacol., 44, 533
(1993).
4. Stjernquist, M. et al., Cell Tissue Res., 292, 1
(1998).
5. Yanagisawa, M., et al., Nature
temperatures. Store the
white plates at room temperature. Store the Goat Anti-Rabbit IgG, HRP Conjugate (12-348-MN),
Anti-Nitrotyrosine, 200X (07-752), and BSA , nitrated (13-127) at -20ºC. If required, aliquot
Cell metabolism, 7(4) 348-356 (2008).
4. Altomare, D.A., and Testa, J.R., Perturbations of
the AKT signaling pathway in human cancer.
Oncogene, 24(50), 7455-7464 (2005).
5. Cicenas, J., The potential
intracellular ion background
of 200 mM K+, 2.0 mM Mg2+, 0.001 mM Ca2+): log aNa ~-2.5
Electrical resistance, tip diameter ~2 µm: ~1010 Ω
Response time: 90% response time ≤5 s
Electrode Characteristics
down-
regulates trafficking of ceramide in the synthesis of
sphingomyelin. Mol. Biol. Cell, 20(1), 348-57
(2009).
PRECISIO is a registered trademark of Sigma-Aldrich
Co. LLC.
RC,MAM 10/11-1
Epigenetics and
human disease: translating basic biology into
clinical applications. CMAJ, 174, 341-348 (2006).
Imprint is a registered trademark of Sigma-Aldrich
Biotechnology LP and Sigma-Aldrich Co
) Tumor invasion and metastasis: role of the
basement membrane. Am. J. Pathol. 117, 339-348
5
Warranty
EMD Millipore Corporation (“EMD Millipore”) warrants
Transport System in
Mammalian Peroxisomes and its Characterization.
Biochem. Biophys. Res. Commun., 348(4),
1224-31 (2006).
3. Richardson, C.L. et al., Isocitrate Ameliorates
Anemia by Suppressing
Harbor, NY), pp. 346-348 (1988).
4. Bergmeyer, H.-U. et al., Methods of Enzymatic
Analysis, 2nd ed. (Bergmeyer, H.-U., ed.). Verlag
Chemie/Academic Press, pp. 1205-1227 (1974).
5. Bernt, E. and
assays.
1. Prepare a 10 mM solution of H202 by
diluting 4 L of H202 Standard (0.88 M)
with 348 L of purified water, mix well.
2. Prepare a 1 mM H202 solution by diluting
100
Harbor, NY), pp. 346-348 (1988).
4. Bergmeyer, H.-U. et al., Methods of Enzymatic
Analysis, 2nd ed. (Bergmeyer, H.-U., ed.). Verlag
Chemie/Academic Press, pp. 1205-1227 (1974).
5. Bernt, E. and Bergmeyer
Harbor, NY), pp. 346-348 (1988).
4. Bergmeyer, H.-U. et al., Methods of Enzymatic
Analysis, 2nd ed. (Bergmeyer, H.-U., ed.). Verlag
Chemie/Academic Press, pp. 1205-1227 (1974).
5. Bernt, E. and Bergmeyer
expressed in CHO cells).
Figure 5. Rate of inactivation the hKv3.3 channel.
Typical current trace of hKv3.3 channel stepped to +30 mV from a holding potential of –80
mV for 200 ms. Time course of inactivation
overlapping dam- methylation (°), but is
sensitive to 5-methyl and 5-hydroxymethylcytosine, as indi-
cated (*).
Storage buffer 20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA, 10 mM 2-Mercap-
toethanol, 0.01%
between estradiol
and progesterone in cycles with luteal phase
deficiency. Fertil. Steril., 1982; 37: 348-354.
12. Kirschner, M.A., The role of hormones in the
etiology of human breast cancer. Cancer
just before use. Do not store the diluted standards.
Dilute 4 µL of 0.88 M H2O2 Standard with 348 µL of
water to prepare a 10 mM H2O2 standard solution.
Dilute the 10 mM H2O2 standard further to