are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM Dithiothreitol, 1 mM
are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 ml by incu-
bation for 16 h at 25°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM dithiothreitol, 1 mM
are ligated with 1 U T4-DNA
ligase (Cat. No. 10 481 220 001) in a volume of 10 �l by
incubation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM
MgCl2, 5 mM Dithiothreitol, 1 mM ATP
are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4 °C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM dithiothreitol, 1 mM
are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4° C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM Dithiothreitol, 1 mM
are ligated with 1 U T4 DNA
Ligase (Cat. No. 10 481 220 001) in a volume of 10 �l by
incubation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM
MgCl2, 5 mM Dithiothreitol, 1 mM ATP
are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 25° C in 66 mM Tris-HCl, 5 mM
MgCl2, 5 mM Dithiothreitol, 1 mM ATP
are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4° C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM Dithiothreitol, 1 mM ATP
are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM Dithiothreitol, 1 mM ATP, pH
are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM Dithiothreitol, 1 mM ATP, pH
are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM dithiothreitol, 1 mM ATP
are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4° C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM dithiothreitol, 1 mM ATP
ligated with 1 unit T4-DNA
ligase (Cat. No. 10 481 220 001) in a volume of 10 �l by
incubation for 16 h at 4°C in 66 mM Tris-HCl,
5 mM MgCl2, 5 mM dithioerythritol, 1 mM ATP, pH
are ligated with 1 U T4-DNA ligase (Cat. No. 10
481 220 001) in a volume of 10 �l by incubation for 16 h
at 4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithioth-
reitol, 1 mM ATP, pH
are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4 °C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM dithiothreitol, 1 mM ATP
�DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 481 220) in a volume of 10 �l by incubation
for 16 h at 4° C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM Dithiothreitol, 1 mM ATP, pH
are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 25°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM Dithiothreitol, 1 mM ATP, pH
are ligated with 1 U T4 DNA
Ligase (Cat. No.10 481 220 001) in a volume of 10 �l by
incubation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM
MgCl2, 5 mM Dithiothreitol, 1 mM ATP, pH
Manta, V. (1990) Gene 92, 1–248.
3 George, J.&Chirikjian, J.G.(1982) Proc. Natl. Acad. Sci. USA 79,
2432.
4 Kolesnikov, V. A. et al. (1981) FEBS Lett. 132, 101.
5 Rebase The Restriction Enzyme
Bron, S. & Murray, K. (1975) Mol. Gen. Genet. 143, 25.
2 Kessler, C. & Manta, V. (1990) Gene 92, 1–248.
3 Rebase The Restriction Enzyme Database:
http://rebase.neb.com
� Ad2 SV40 � X174 M13mp7 pBR322
are ligated with 1 U T4-DNA ligase (Cat.
No. 10 481 220 001) in a volume of 10 �l by incubation
for 16 h at 4 °C in 66 mM Tris-HCl, 5 mM MgCl2, 5 mM
Dithiothreitol, 1 mM ATP