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Nadine Dyballa et al.
Methods in molecular biology (Clifton, N.J.), 893, 47-59 (2012-06-06)
Proteins separated by two-dimensional gel electrophoresis can be visualized by in-gel detection using -different staining methods. Ideally, the dye should bind non-covalently to the protein following a linear response curve. Since protein concentrations in biological systems may vary by six
C Scheler et al.
Electrophoresis, 19(6), 918-927 (1998-06-25)
Identification of proteins separated by two-dimensional electrophoresis (2-DE) is a necessary task to overcome the purely descriptive character of 2-DE and a prerequisite to the construction of 2-DE databases in proteome projects. Matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS) has a
Karel Mazanec et al.
Journal of mass spectrometry : JMS, 41(12), 1570-1577 (2006-12-01)
The use of low-molecular-mass color pI markers for the determination of pI values of proteins in gel isoelectric focusing (IEF) in combination with mass spectrometry is described. Different types of substituted phenols of known pI values within the mass range
Na Na et al.
Methods in molecular biology (Clifton, N.J.), 869, 511-520 (2012-05-16)
Polyacrylamide gel electrophoresis (PAGE) has been one of the most powerful and widely used separation techniques for complex biological samples, whose traditional detection methods include organic dye or silver staining. As a simple, convenient, and ultrasensitive detection of proteins for
Mayara Medeiros de Freitas Carvalho et al.
Scientific reports, 9(1), 8107-8107 (2019-05-31)
Non-alcoholic fatty liver disease (NAFLD), the most predominant liver disease worldwide, is a progressive condition that encompasses a spectrum of disorders ranging from steatosis to steatohepatitis, and, ultimately, cirrhosis and hepatocellular carcinoma. Although the underlying mechanism is complex and multifactorial
S Fazekas de St Groth et al.
Biochimica et biophysica acta, 71, 377-391 (1963-05-14)
Two new procedures are described for the estimation of protein by direct photometry on electrophoretic strips. The protein complexes of procion brilliant blue RS and coomassie brilliant blue R250 are shown to follow Beer's law up to 50 and 20
Veronica Zaga-Clavellina et al.
Reproductive biology and endocrinology : RB&E, 9, 13-13 (2011-01-27)
Premature rupture of fetal membranes (PROM) complicated with intrauterine infection has been associated to alterations of the extracellular matrix (ECM) homeostasis. The aim of this work was to evaluate the integral/functional response of the amnion (AMN) and choriodecidua (CHD) to
One-step Coomassie brilliant blue R-250 staining of proteins in polyacrylamide gel.
H Chen et al.
Analytical biochemistry, 212(1), 295-296 (1993-07-01)
Quantitation of protein on gels and blots by infrared fluorescence of Coomassie blue and Fast Green.
Shen Luo et al.
Analytical biochemistry, 350(2), 233-238 (2005-12-13)
Coomassie blue staining of gels and blots is commonly employed for detection and quantitation of proteins by densitometry. We found that Coomassie blue or Fast Green FCF bound to protein fluoresces in the near infrared. We took advantage of this
E Zeindl-Eberhart et al.
Electrophoresis, 18(5), 799-801 (1997-05-01)
A new, easy method for the immunodetection of specific antigens in two-dimensional electrophoresis (2-DE) is described. Areas of 2-DE gels containing antigens of interest are electrophoretically transferred to polyvinylidene difluoride membranes, immunostained with specific antibodies using Fast Red or 5-bromo-4-chloro-3-indolyl
Joseph M Tuscano et al.
Cancer immunology, immunotherapy : CII, 60(6), 771-780 (2011-02-25)
Previous studies have shown that bispecific antibodies that target both CD20 and CD22 have in vivo lymphomacidal properties. We developed a CD20-CD22 bispecific antibody (Bs20x22) from anti-CD20 and the anti-CD22 monoclonal antibodies (mAb), rituximab and HB22.7, respectively. Bs20x22 was constructed
Tomohito Kameda et al.
Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering, 47(13), 2035-2039 (2012-08-09)
The objective of this study is to evaluate the capacity of Mg-Al layered double hydroxide (Mg-Al LDH) intercalated with organic dye to adsorb aromatic compounds in aqueous solutions. Mg-Al LDH intercalated with Coomassie Brilliant Blue R anion (BB(-)) was prepared
Jerome C Servaites et al.
Analytical biochemistry, 421(1), 75-80 (2011-12-06)
Protein is a large component of the standing biomass of algae. The total protein content of algae is difficult to measure because of the problems encountered in extracting all of the protein from the cells. Here we modified an existing
Zhang Xin-Guo Chen Jian-Hua et al.
Journal of chromatographic science, 50(9), 820-825 (2012-06-22)
A human serum albumin and Thymosin α1 (HSA-Tα1) fusion protein was designed and over-expressed in Pichia pastoris. To purify the fusion protein, a new native preparative electrophoresis system that involved a modified device with a sample receiving chamber, and an
Jintae Lee et al.
Applied and environmental microbiology, 75(6), 1703-1716 (2009-01-27)
SdiA is a homolog of quorum-sensing regulators that detects N-acylhomoserine lactone (AHL) signals from other bacteria. Escherichia coli uses SdiA to reduce its biofilm formation in the presence of both AHLs and its own signal indole. Here we reconfigured SdiA
Reiner Westermeier
Proteomics, 6 Suppl 2, 61-64 (2006-10-13)
In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There
Chun Yi Liau et al.
Journal of bioscience and bioengineering, 106(1), 111-113 (2008-08-12)
A modified Coomassie Brilliant Blue G 250 staining method for detecting chitinolytic enzymes in chitin-containing polyacrylamide gel electrophoresis (PAGE) is presented. The staining formed achromatic zones at the locations of the migrated enzyme. Using Streptomyces griseus chitinase, we have demonstrated
High-efficiency staining of proteins on different blot membranes.
C R Yonan et al.
Analytical biochemistry, 338(1), 159-161 (2005-02-15)
Virginia Caponera et al.
Journal of economic entomology, 113(1), 348-352 (2019-10-08)
Damage from termite infestations is economically significant and control can be costly when requiring the widespread use of conventional insecticides. Erythritol, a polyalcohol sweetener that is safe for human consumption, causes increased mortality when ingested by some insects, indicating potential
Montse Olivé et al.
Neuromuscular disorders : NMD, 18(12), 929-933 (2008-10-25)
A 27-year-old woman of Moldavian origin presented at the age of 15 with progressive proximal limb weakness and painful cramps in her calf muscles. Clinical examination revealed prominent muscle weakness in proximal muscles of the lower extremities and distal anterior
Elias Leiva-Salcedo et al.
Mediators of inflammation, 2011, 152625-152625 (2011-09-24)
The purinergic P2X7 receptor (P2X7R) plays an important role during the immune response, participating in several events such as cytokine release, apoptosis, and necrosis. The bacterial endotoxin lipopolysaccharide (LPS) is one of the strongest stimuli of the immune response, and
Pierrot Lundimu Tugirimana et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 24(5), 1485-1490 (2008-12-20)
A simple spot test was developed, which allows quantification of microalbuminuria. Evaluation was carried out according to the ISO 15189 guidelines. Urine was spotted on cellulose acetate strips and stained using different sensitive protein binding dyes (nigrosin, Coomassie Blue R-250
Jiafa Ren et al.
Journal of the American Society of Nephrology : JASN, 30(9), 1674-1685 (2019-07-19)
Following an acute insult, macrophages regulate renal fibrogenesis through the release of various factors that either encourage the synthesis of extracellular matrix synthesis or the degradation of matrix via endocytosis, proteolysis, or both. However, the roles of infiltrating versus resident
K Kubo
Analytical biochemistry, 213(2), 200-205 (1993-09-01)
In sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) according to the method of Weber and Osborn, protein bands are often distorted by tailing at both ends, and the phenomenon is often called "edge tailing." This was eliminated by adding glycerol at
J A Beeley et al.
Electrophoresis, 17(3), 505-506 (1996-03-01)
Detection of human parotid salivary proteins by dansylation and UV-transillumination after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) has been compared with Coomassie Blue R-250 and silver staining procedures. Dansylation gives superior results in terms of both resolution and sensitivity, especially
D Lee et al.
European journal of biochemistry, 268(2), 295-301 (2001-02-13)
alpha-Synuclein has been implicated in various neurodegenerative disorders, including Parkinson's and Alzheimer's diseases, by its participation in abnormal protein depositions. As the protein has been suggested to play a significant role in the formation of the deposits which might be
G Sreeramulu et al.
Electrophoresis, 16(3), 362-365 (1995-03-01)
A novel method for destaining of polyacrylamide gels, stained with Coomassie Brilliant Blue R-250, is described, based on the use of 0.5 M NaCl in water as the destainer, requiring only 2-3 h. Concentrated (> 2 M) or dilute (<
David Choveaux et al.
Methods in molecular biology (Clifton, N.J.), 869, 585-589 (2012-05-16)
Proteins separated by SDS-polyacrylamide gel electrophoresis need to be stained with organic dyes to be visualized and to enable comparisons to be made between the intensity of protein bands to observe and determine differences in protein concentration. The standard protein
A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis.
A Chrambach et al.
Analytical biochemistry, 20(1), 150-154 (1967-07-01)
Jie Zang et al.
Journal of experimental botany, 71(19), 5896-5910 (2020-07-21)
Iron (Fe) is an essential micronutrient and plays an irreplaceable role in plant growth and development. Although its uptake and translocation are important biological processes, little is known about the molecular mechanism of Fe translocation within seed. Here, we characterized
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