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Konstantin Chingin et al.
Analytical chemistry, 84(15), 6856-6862 (2012-07-12)
We introduce an online multiple-junction capillary isoelectric focusing fractionator (OMJ-CIEF) for separation of biological molecules in solution by pI. In OMJ-CIEF, the separation capillary is divided into seven equal sections joined with each other via tubular Nafion membrane insertions. Each
Hanzhi Wu et al.
Talanta, 131, 640-644 (2014-10-05)
Biologically active peptides play a role in plant signaling and defense. Elderberry juice is known to contain a variety of anthocyanin compounds, a sub-set of polyphenols, which are responsible for the deep purple color of the juice. In this paper
Michael Boppré et al.
Phytochemical analysis : PCA, 26(3), 215-225 (2015-02-04)
The freshwater aquatic plant Gymnocoronis spilanthoides (Senegal tea plant, jazmín del bañado, Falscher Wasserfreund) is an invasive plant in many countries. Behavioural observations of pyrrolizidine alkaloid-pharmacophagous butterflies suggested the presence of pyrrolizidine alkaloids in the plant. To determine whether the
Dawei Chen et al.
Journal of agricultural and food chemistry, 63(44), 9855-9862 (2015-10-22)
A novel dispersive micro solid phase extraction (DMSPE) method based on a polymer cation exchange material (PCX) was applied to the simultaneous determination of the 30 triazine herbicides in drinking water with ultrahigh-performance liquid chromatography-high-resolution mass spectrometric detection. Drinking water
Zheng Wang et al.
Analytica chimica acta, 725, 81-86 (2012-04-17)
A modified SBA-15 mesoporous silica material NH(2)-SBA-15 was synthesized successfully by grafting γ-aminopropyl-triethoxysilane. The material was characterized using transmission electron microscopy (TEM) and Fourier transform infrared/Raman (FT-IR/Raman) spectroscopy, and used for the first time in a flow injection on-line solid
Stefan Harmsen et al.
Nature protocols, 12(7), 1400-1414 (2017-07-08)
The unique spectral signatures and biologically inert compositions of surface-enhanced resonance Raman scattering (SERRS) nanoparticles make them promising contrast agents for in vivo cancer imaging. Our SERRS nanoparticles consist of a 60-nm gold nanoparticle core that is encapsulated in a
S A Hassanzadeh-Tabrizi et al.
Materials science & engineering. C, Materials for biological applications, 58, 737-741 (2015-10-20)
In the present study, forsterite nanoparticles were synthesized via surfactant-assisted sol-gel method using cetyltrimethyl ammonium bromide (CTAB) as a surfactant. The effects of CTAB contents and heat treatment on the textural properties and drug release from nanoparticles were investigated. The
Shinji Yamashita et al.
Lipids, 49(12), 1261-1273 (2014-11-12)
We have developed a new method for determining ethanolamine plasmalogen contents in marine invertebrates. This quantification method involves derivatization of ethanolamine glycerophospholipid (EtnGpl) subclasses, alkenylacyl (plasmalogen), diacyl, and alkylacyl subclasses, by enzyme treatment and acetylation, followed by separation and detection
Bradley Duncan et al.
Small (Weinheim an der Bergstrasse, Germany), 11(11), 1302-1309 (2014-11-11)
An effective method for the generation of hybrid organic-inorganic nanocomposite microparticles featuring controlled size and high structural stability is presented. In this process, an oil-in-water Pickering emulsion is formed using hydrophilic amine-functionalized silica nanoparticles. Covalent modification using a hydrophobic maleic
Chiang-Jui Chu et al.
Macromolecular rapid communications, 35(18), 1598-1605 (2014-08-08)
Block copolymers have been extensively studied over the last few decades because they can self-assemble into well-ordered nanoscale structures. The morphologies of block copolymers in confined geometries, however, are still not fully understood. In this work, the fabrication and morphologies
Mina Mirzaian et al.
Journal of lipid research, 56(4), 936-943 (2015-01-30)
Sulfatides are found in brain as components of myelin, oligodendrocytes, and neurons but are also present in various visceral tissues. Metachromatic leukodystrophy (MLD) is an inherited lysosomal storage disorder caused by a deficiency of arylsulfatase A, leading to severe white
Ramsey I Zeitoun et al.
Lab on a chip, 11(23), 4022-4028 (2011-10-15)
Nanolitre droplets in microfluidic devices can be used to perform thousands of independent chemical and biological experiments while minimizing reagents, cost and time. However, the absence of simple and versatile methods capable of controlling the contents of these nanolitre chemical
Mir Mahdi Abolghasemi et al.
Journal of chromatography. A, 1381, 48-53 (2015-01-27)
In the present study, 12-tungstophosphoric (PW) acid as heteropoly acid, supported on silica-coated γ-Fe2O3 nanoparticles (NPs), was used as a new fiber coating for solid-phase microextraction (SPME). The γ-Fe2O3@SiO2-PW nanocomposite with high surface area was synthesized and characterized by SEM
Qi Ge et al.
American journal of physiology. Lung cellular and molecular physiology, 309(10), L1093-L1102 (2015-09-06)
Altered ECM protein deposition is a feature in asthmatic airways. Fibronectin (Fn), an ECM protein produced by human bronchial epithelial cells (HBECs), is increased in asthmatic airways. This study investigated the regulation of Fn production in asthmatic or nonasthmatic HBECs
Shengqiang Tong et al.
Journal of chromatography. A, 1374, 77-84 (2014-12-06)
Spiral tube assembly counter-current chromatography was successfully applied in enantioseparation of dl-tryptophan using bovine serum albumin as chiral selector. An improved biphasic aqueous-aqueous solvent system 12.0% (w/w) polyethyleneglycol 8000-9.0% (w/w) dibasic potassium phosphate-0.1% ammonia-78.9% water was used as the solvent
Chao Yu et al.
Journal of hazardous materials, 283, 519-528 (2014-12-03)
Manganese oxide nanoparticles (MnO NPs) have been regarded as a new class of T1-positive contrast agents. The cytotoxicity of silica coated MnO NPs (MnO@SiO2 NPs) was investigated in human cervical carcinoma cells (HeLa) and mouse fibroblast cells (L929). The changes
Sandrine Aros-Calt et al.
Journal of proteome research, 14(11), 4863-4875 (2015-10-27)
Staphylococcus aureus can cause a variety of severe disease patterns and can readily acquire antibiotic resistance; however, the mechanisms by which this commensal becomes a pathogen or develops antibiotic resistance are still poorly understood. Here we asked whether metabolomics can
Levi B Wood et al.
Scientific reports, 5, 16622-16622 (2015-11-14)
Alzheimer's disease (AD) therapeutics based on the amyloid hypothesis have shown minimal efficacy in patients, suggesting that the activity of amyloid beta (Aβ) represents only one aspect of AD pathogenesis. Since neuroinflammation is thought to play an important role in
Jianshe Wang et al.
Journal of proteome research, 14(1), 330-341 (2014-09-03)
Perfluoroalkyl chemicals (PFASs) are a class of highly stable man-made compounds, and their toxicological impacts are currently of worldwide concern. Administration of perfluorononanoic acid (PFNA), a perfluorocarboxylic acid (PFCA) with a nine carbon backbone, resulted in dose-dependent hepatomegaly in mice
James P Grinias et al.
Journal of chromatography. A, 1370, 270-273 (2014-12-03)
Reducing the particle size distribution (PSD) of sub-2 μm chromatographic packing materials can improve the performance of capillary UHPLC columns, but several size refinement methods are only partially effective in this size range. To this end, a preparative scale hydrodynamic
Mahdi Shahmiri et al.
Scientific reports, 5, 16378-16378 (2015-11-18)
Aurein 1.2 is a 13 residue antimicrobial peptide secreted by the Australian tree frog Litoria Aurea. It is a surface-acting membrane disrupting peptide that permeabilizes bacterial membranes via the carpet mechanism; the molecular details of this process are mostly unknown.
A Farag et al.
Journal of dental research, 93(12), 1313-1319 (2014-10-02)
The periodontal ligament is the key tissue facilitating periodontal regeneration. This study aimed to fabricate decellularized human periodontal ligament cell sheets for subsequent periodontal tissue engineering applications. The decellularization protocol involved the transfer of intact human periodontal ligament cell sheets
Bat-Chen Cohen et al.
PloS one, 10(3), e0121645-e0121645 (2015-03-11)
Milk fat globule size is determined by the size of its precursors-intracellular lipid droplets-and is tightly associated with its composition. We examined the relationship between phospholipid composition of mammary epithelial cells and the size of both intracellular and secreted milk
F Munarin et al.
International journal of biological macromolecules, 72, 199-209 (2014-08-12)
Pectin-based biocomposite hydrogels were produced by internal gelation, using different hydroxyapatite (HA) powders from commercial source or synthesized by the wet chemical method. HA possesses the double functionality of cross-linking agent and inorganic reinforcement. The mineralogical composition, grain size, specific
Youngjoo Kwon et al.
PloS one, 6(4), e18872-e18872 (2011-04-29)
Epithelial ovarian cancer is an aggressive and deadly disease and understanding its invasion mechanisms is critical for its treatment. We sought to study the penetration/invasion of ovarian tumor cells into extracellular matrices (ECMs) using a fibroblast-derived three-dimensional (3D) culture model
Kui Cheng et al.
Colloids and surfaces. B, Biointerfaces, 126, 387-393 (2014-12-17)
Design of an implant surface with appropriate nanostructures has been considered to be an effective way to promote osteoblast cell growth and osseointegration. In this work, TiO2 nanorods were integrated into TiO2 nanodot films by hydrothermal growth to engineer a
Ruben Magni et al.
Journal of translational medicine, 13, 346-346 (2015-11-06)
Prompt antibiotic treatment of early stage Lyme borreliosis (LB) prevents progression to severe multisystem disease. There is a clinical need to improve the diagnostic specificity of early stage Lyme assays in the period prior to the mounting of a robust
Sarah E Stewart et al.
Biochimica et biophysica acta, 1848(1 Pt A), 115-126 (2014-10-15)
Streptolysin O (SLO) is a bacterial pore forming protein that is part of the cholesterol dependent cytolysin (CDC) family. We have used quartz crystal microbalance with dissipation monitoring (QCM-D) to examine SLO membrane binding and pore formation. In this system
Michael A Gillette et al.
Cell, 182(1), 200-225 (2020-07-11)
To explore the biology of lung adenocarcinoma (LUAD) and identify new therapeutic opportunities, we performed comprehensive proteogenomic characterization of 110 tumors and 101 matched normal adjacent tissues (NATs) incorporating genomics, epigenomics, deep-scale proteomics, phosphoproteomics, and acetylproteomics. Multi-omics clustering revealed four
Blanca Estela Jaramillo-Loranca et al.
Synapse (New York, N.Y.), 69(4), 213-225 (2015-02-17)
Sigma (σ) receptors have generated a great deal of interest due to their possible role in psychosis, neuroprotection, and various other behaviors including addictive processes. Sigma receptors have been located in brain areas involved in motor functions, including the dopaminergic
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