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2. Jefferson, R.A., et al., GUS fusions: beta-glucuronidase as a sensitive
and versatile gene fusion marker in higher plants. EMBO J. 6, 3901
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(1998).
12. Szabo, C. et al., J. Exp. Med., 186, 1041 (1997).
13. Mizumoto, K., and Farber
diluent (for reduced background add 1-5% BSA to diluent) for 60 minutes.
5. Rinse 3x3 minutes each with PBS.
6. Incubate with primary antisera diluted in diluent 12-24 hours (longer incubations may give
Acad. Sci. USA 98, 12438
p53 (1-342, C-Terminal Deletion), His•Tag®,
Human, Recombinant, S. frugiperda
(p53 del 1-342)
A human p53 mutant protein (amino acids 1-342) with
deletion of the C-terminal
Semin Cell Biol 5:165-174.
5. Rao JY et al. (1990). Cancer Res 50:2215-2220.
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7. Burridge K et al. (1990). Cell Diff Dev 32:337-342.
8. Turner
traces of amplicons in the size range of 250-400 bp (the theoretical fragment sizes are: 287, 324,
342, 364 and 370 bp) See graph below.
Example of the purified library of the 5R-PLEX positive control
Wein-
heim and New York.
4 Müller-Wiefel, D. E. et al. (1978) Monatsschr. Kinderheilkd. 126,
342-343.
5 Müiller-Wiefel, D. E. et al. (1978) Eur. J. Pediatr. 128,103-111.
Changes to
previous version
volume of fresh pre-warmed
complete medium to cells and incubate for an
additional 24 hours
Day 5
Second harvest of Viral Particles
22. Between 60-72 hours post-transfection collect viral
volume of fresh pre-warmed
complete medium to cells and incubate for an
additional 24 hours
Day 5
Second harvest of Viral Particles
22. Between 60-72 hours post-transfection collect viral
Fedière, G. (1990). A new serum-free medium
for lepidopteran cell culture. J. Invertebr. Pathol. 55, 342-349.
13. DiSorbo, D.M, Whitford, W.G, and Weiss, S.A. (1991).
Accessory reagents to enhance quantification
. Cell Physiol. 180, 263.
23. Wieder, T., et al. 1997. FEBS Lett. 411, 260.
24. Bektas, M., et al. 1998. Exp. Dermatol. 7, 342.
25. Murohara, T., et al. 1996. Circ. Res. 78, 780.
26. Masamune, A., et al
the nicotinic acetylcholine receptor in
the rat central nervous system. J. Comp. Neurol. 349:325-342.
Duvoisin RM, Deneris ES, Patrick J, Heinemann S (1989) The functional diversity of the
neuronal
-45 minutes at room temperature.
5. Add entire transfection cocktail volume to the
corresponding flask(s) containing HEK293T cells.
6. Incubate at 37 °C for 12-24 hours.
Day 3
Change media
7. Pre-warm
error). The DNA
Labeling Solution is active for approximately 24 hours.
Antibody Solution Catalog No. (Color code) 1 Assay 5 Assays 10 Assays
Anti-BrDU-Fluorescein F 6175(orange cap) 5.00
Antibody to all tubes except Total Count tubes (1-2) and NSB tubes
(3-4).
5. Vortex, cover, and incubate overnight (20-24 hours) at 4 C.
Day Two
6. Hydrate the
7: 329-342.
4. Buecker C, et al. (2010) A murine ESC-like state facilitates
transgenesis and homologous recombination in human
pluripotent stem cells. Cell Stem Cell 6: 535-546.
5. Huangfu
should be
decontaminated in 10% bleach before disposal.
5. Place plate in an incubator at 37 °C in a humidified
5% CO2 atmosphere for 24 hours.
Day 3 Selection
1.
should be
decontaminated in 10% bleach before disposal.
5. Place plate in an incubator at 37 °C in a humidified
5% CO2 atmosphere for 24 hours.
Day 3 Selection
1.
nonMurine research use requires a license from Cell Genesys, Inc.
Please contact Cell Genesys, Inc. at 342 Lakeside Drive, Foster
City, California 94404. Use of this technology to make or sell
products or
nonMurine research use requires a license from Cell Genesys, Inc.
Please contact Cell Genesys, Inc. at 342 Lakeside Drive, Foster
City, California 94404. Use of this technology to make or sell
products or