hydroxide solution 50% for analysis EMSURE® 1.58793.9200
Sodium hydroxide solution about 32% (for the determination of nitrogen) for analysis EMSURE® 1.05590.9200
Sodium hydroxide solution about 32% EMPLURA® 1.05587.9200
5.14 4.89 105
1:8 1.18 1.25 97
1:16 0.49 0.59 83
Specificity
• The Phospho-IκBα [pSer32] ELISA is specific for
measurement of human IκBα, which is
phosphorylated on serine 32.
• The
removal of
the fitting on the injector or detector.
• Option 2: female M6/male 10-32 fitting to
female 10-32/female 10-32 fitting two-piece
adapter (Catalog Number 55068) – A two-piece
adapter can be
removal of
the fitting on the injector or detector.
• Option 2: female M6/male 10-32 fitting to
female 10-32/female 10-32 fitting two-piece
adapter (Catalog Number 55068) – A two-piece
adapter can be
removal of
the fitting on the injector or detector.
• Option 2: female M6/male 10-32 fitting to
female 10-32/female 10-32 fitting two-piece
adapter (Catalog Number 55068) – A two-piece
adapter can be
µl Lysis Solution + 32 µl 5M NaCl Pulse vortex.
8. Incubate at 65 °C for 5 min.
9. Incubate at room temperature for 5 min.
10. Spin for 2 min to pellet beads. Discard all but
~50 µl supernatant.
removal of
the fitting on the injector or detector.
• Option 2: female M6/male 10-32 fitting to
female 10-32/female 10-32 fitting two-piece
adapter (Catalog Number 55068) – A two-piece
adapter can be
of 3 times of 10 minutes each.
8. Set up a radioactive control to measure the total
-
32
P-ATP counts introduced into the reaction. Spot
5 l of the -
32
P-ATP Assay Cocktail