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Milliflex Oasis User Guide
并用无菌
涂布棒将接种物涂抹在培养基上。
说明：建议在培养皿涂抹不超过50 μL。
为了保持方法一致，必须在对照培养皿上涂布
相同体积。
• 用漏斗盖盖住接种后的培养皿。
• 在指定的温度和时间孵育培养皿。
说明：孵育培养皿，盖子朝下。
第25 / 32页
8. Milliflex® 预制培养皿
可以使用这些产品（老一代）代替Milliflex
Aldrich Polymer Products Application - Ionx
-100 8 G 50-100 Cl 46 66 1.2 3.5 0-14
21,742-5 1X8-200 8 G 100-200 Cl 46 66 1.2 3.5 0-14
21,743-3 1X8-400 8 G 200-400 Cl 46 66 1.2 3.5 0-14
42,876-0 MSA-1 - Mp 20-50 Cl 60 66 1.0 4.0 0-14
43,665-8
Aldrich Polymer Products Application - Transitions
napthalenedicarboxylate 113
Ethylene oxide -66 66
Ethylene terephthalate 72 265
2-Ethylhexyl acrylate -50
2-Ethylhexyl methacrylate -10
2-Ethylhexyl vinyl ether -66
Ethyl methacrylate 65
Ethyl vinyl ether
Data Sheet - I5646
7041 (1992).
2. Devos, R., et al., EMBO J., 8, 2133, (1991).
3. Tavernier, J., et al., Cell, 66, 1175 (1991).
4. Kitamura, T., et al., Cell, 66, 1165 (1991).
11/98
Sigma brand
Data Sheet - R6634
dithiothreitol
200 µg/ml bovine serum albumin
50% (v/v) glycerol
pH 8.3
1x Digestion Buffer SA (B 7531) Composition for Alw
44 I: 100 % Digestion at 37 °C.
33 mM Tris-acetate
66 mM Potassium acetate
10 mM Magnesium
POST-TRANSLATIONAL MODIFICATIONS
Anti-Phosphotyrosine Agarose, clone PT-66
A 8076 Monoclonal Anti-Phosphoserine Agarose, clone PSR-45
A 7951 Monoclonal Anti-Phosphothreonine Agarose, clone PTR-8
Product
Code Name Description
The Effective Use of Protein Kinase Inhibitors
22 94 57 15 53 45 65 75 93 43 59 97 53 74 23
96 102 60 71 50 30 22 1 95 59 6 72 36 93 84 94 14 26 90 101 82 61
48
Data Sheet - R6004
v) Triton X-100
50% (v/v) glycerol
pH 8.0
Quality Control Testing
1x Digestion Buffer SA (B 7531) Composition
for Ava II: 100% Digestion at 37 °°°°C.
33 mM Tris-acetate
66 mM Potassium acetate
Anti-Cyclin E antibody produced in rabbit (C4976)
fixative (50% ethanol, 50% acetic acid) for 1
min. at room temperature.
4. Wash the cells with PBS, 2 X for 15 min. with
gentle agitation. Do not shake cells.
5. Add 400 µl PBS containing
Impurities & Related Compounds: Reference Materials
106-47-8
50-63-5
86-98-6
113-92-8
120244-82-8
1246816-57-8
1202-34-2
22630-25-7
67-97-0
434-16-2
29342-05-0
41621-49-2
14818-35-0
73963-72-1
54197-66-9
73963
Data Sheet - P7997
of mouse KIR3.1
(GIRK1) as the immunogen. This sequence is identical
in rat, and has 64/66, 63/66, and 59/66 residues
identical in human, guinea pig, and chicken,
respectively. The antibody was affinity
The Effective Use of Protein Kinase Inhibitors
22 94 57 15 53 45 65 75 93 43 59 97 53 74 23
96 102 60 71 50 30 22 1 95 59 6 72 36 93 84 94 14 26 90 101 82 61
48
Product Information Sheet-04693132001
11 734 334 001
Pepstatin 2 mg 10 253 286 001
10 mg 11 359 053 001
50 mg 11 524 488 001
1 2 3
1 2 3
03
21
.0
47
28
66
10
01
Data Sheet - 11012
Clin. Pathol., 4, 66 (1951)
7. E.J.L. Lowbury, A.G. Collins, The use of a new cetrimide product in a selective medium for Pseudomonas
pyocyanea, J. Clin. Pathol., 8, 47 (1955)
8. X. Bühlmann, W.A.
Product Information Sheet - A9285
been
reported.
8
The crystal structure of uncleaved wild-type
AACT, expressed recombinantly, has been published.
9
Purified AACT loses its inhibitory activity above 50 C,
and below pH
Data Sheet - R4506
pH 7.5. A 10 µl reaction mixture, incubated for
16 hrs. at 4 °C, contained: 0.1 unit T4-DNA ligase,
66 mM Tris-HCl, 5 mM MgCl2, 1 mM dithioerythritol and
1 mM ATP.
The degree of ligation and subsequent
Data Sheet - I17001
mouse leukemia L
1210 cells in vitro by interferon preparations. Proc.
Natl. Acad. Sci. U. S. A., 66, 1052-8 (1970).
4. Knight, E., Jr., Antiviral and cell growth inhibitory
activities reside in the
Product Information Sheet - C7150
• Albumin, bovine serum, Catalog
Number A8531, 50 mg/vial
(Contains ∼0.3% dithiothreitol)
66 kDa
• Blue Dextran, Catalog Number
D4772, 50 mg/vial
2,000 kDa
Precautions and Disclaimer
Product Information Sheet - A8531
• Albumin, bovine serum, Catalog
Number A8531, 50 mg/vial
(Contains ∼0.3% dithiothreitol)
66 kDa
• Blue Dextran, Catalog Number
D4772, 50 mg/vial
2,000 kDa
Precautions and Disclaimer
Data Sheet - 91019
sporogenes (11437/-) 50-100 +++ ≥50%
Escherichia coli (25922/00013) ≥104 - 0%
References:
1. Schaedler R. W., Dubos R. and Castello R. (1965) J. Exp. Med. 122. 59-66.
2. Mata L. J., Carrillo C. and
Data Sheet - R8257
10 µl reaction mixture, incubated
for 16 hrs. at 20 °C contained 0.5 units T4-DNA ligase,
66 mM Tris-HCl, 5 mM MgCl2, 5 mM dithioerythritol,
1
Data Sheet - H9773
21-26 (1987).
8. Van de Rhee, M.D. et al., Mol. Gen. Genet., 250,
252-258 (1996).
9. Perl, A. et al., Nat. Biotechnol., 14, 624-628 (1996).
10. Woods, J.P. et al, Infect. Immun., 66, 1697-1707
(
Data Sheet - P5872
al., J. Biol. Chem., 274, 18141-18144
(1999).
8. Loster, K. et al., Biochem. J., 356, 233-240 (2001).
9. Bern, O. et al., FEBS Lett., 403, 45-50 (1997).
10. Volberg, T. et al., J. Cell Sci., 114
Product Information Sheet - A3886
• Albumin, bovine serum, Catalog
Number A8531, 50 mg/vial
(Contains ∼0.3% dithiothreitol)
66 kDa
• Blue Dextran, Catalog Number
D4772, 50 mg/vial
2,000 kDa
Precautions and Disclaimer
Product Information Sheet - C7025
• Albumin, bovine serum, Catalog
Number A8531, 50 mg/vial
(Contains ∼0.3% dithiothreitol)
66 kDa
• Blue Dextran, Catalog Number
D4772, 50 mg/vial
2,000 kDa
Precautions and Disclaimer
Data Sheet - A6191
et al., Antipain, a new protease inhibitor
isolated from actinomycetes., J. Antibiot., 25,
263-66 (1972).
3. Umezawa, H., Structures and activities of protease
inhibitors of microbial origin.
Application Note - RNA Isolation from FFPE Tissues
20 25 30 35 40 45 50 55 60 65
Fl
uo
re
sc
en
ce
Time (seconds)
Fl
uo
re
sc
en
ce
Ti
m
e
(s
ec
on
ds
)
Time (seconds)
1 2
Data Sheet - B7150 - Lot 61K8804
Storage 2-8°C; After opening, transfer contents to
small vial provided for your convenience.
Mattson Galaxy 3020 FT-IR
70 eV Electron Ionization Mass Spectrum
40 50 60 70 80
Product Information - 452882
9125
Product Description
Molecular Formula: NaBH4
Molecular Weight: 37.83
CAS Number: 16940-66-2
Sodium borohydride is a white crystalline powder,
packaged in a glass bottle. The bottle is
Data Sheet - R0629
C. A 10 µl reaction mixture, incubated
for 16 hrs. at 20 °C, contained 0.5 unit T4-DNA ligase,
66 mM Tris-HCl, 5 mM MgCl2, 1mM dithiothreitol and
1 mM ATP.
The degree of ligation and
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