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60-24-2
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关键词:'60-24-2'
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Product Information Sheet - MAK468
Working Reagent according to Table 2. Fresh
reconstitution is recommended.
Table 2.
Preparation of Working Reagent
Reagent Working Reagent
Assay Buffer 60 µL
Enzyme A 1 µL
Micropure-EZ Enzyme Removers
Removers 8 42528
24 42549
100 42530
Micropure-EZ Enzyme Removers 8 42531
Filter units only (for use with Microcon) 24 42532
100 42533
Microcon Centrifugal UF Filter Devices
(includes 2 vials with
Cell Culture Poster
68.6 101.6 53.8 47.9 1.2 101.1
0
20
40
60
80
100
120
140
160
180
200
n=9 n=2 n=2
3D Insert Cell Seeding Protocol
Size
PS1520 PS3040 PCL3030 PCL3050
96‐well 15 20 20 25
48‐well 25 50 50 70
24‐well 60 120 130 160
12‐well 120 240 270 340
6‐well 300 600 690 880
4. Gently place
Amicon® Ultra-2 Centrifugal Filter Devices User Guide
137 15 80 25 879 2
10 731 3 101 21 51 39 30 71 203 10
15 60 33 37 57 22 90 61 34
20 215 10 39 51 24 85 21 99 32 63
Product Information Sheet - P5475
Dilute a 3 µl aliquot of the C-CMV-24 sequencing
primer with 6 µl of 1X TE to make a final
concentration of 1.67 pmol/µl.
2. Add 2-3 µl (3.5-5 pmoles) of C-CMV-24 sequencing
primer to 10 µl (1 pmole
Rotary Evaporator Replacement Glassware
Cat. No.
24/40 210-215 160 48068 Z682411-1EA
29/32 210-215 160 46964 Z682438-1EA
24/40 R114-R144 160 32336 Z682462-1EA
29/32 R114-R144 160 32001 Z682470-1EA
29/42 R114-R144 60 32338 Z682489-1EA
24/40 114-
Poster: Directed Migration of Neural Stem Cells by Angiogenic Factors
40 60
Number of Cells x 1000
80 100 1200
O
pt
ic
al
D
en
sit
y
(5
70
n
M
) y=4E-06x + 0.1497
0
.05
0.1
0.15
0.2
0.25
0.3
MMP Activation Chart
9749
MMP-17 MT4-MMP rProCatDom TrypsinTPCK, 5 µg/ml 25°C; 30-120 min 24 Takino et al., JBC 270/ ’95/ 23013
MMP-24 MT5-MMP 63 nd Intracellularly 40-46 29 Pei
Product Information Sheet - MAK472
Standard
Purified
Water
Iron
(µg/dL)
1 100 µL - 1000
2 80 µL 20 µL 800
3 60 µL 40 µL 600
4 40
Product Information Sheet - SHP002
pre-warmed
complete medium to cells and incubate for an
additional 24 hours
Day 5
Second harvest of Viral Particles
22. Between 60-72 hours post-transfection collect viral
particles by carefully
Application Note: Multi-Column Capture
quantity loaded in batch mode) can roughly
meet a BT of 60%, which represents about 90%
of the resin utilization (instead of 60–70%).
Thus loading 2 columns set in series instead of
one column, allows
Product Information Sheet - MAK530
all components to room temperature prior
to use.
mak530pis Rev 01/24
2
Product Information Sheet-11277073910
Stop the reaction by adding 2 μl of 0.2 M EDTA (pH 8.0).
1
2
3
4
https://www.sigmaaldrich.com
2. How to Use this Product
Product Information Sheet - W2629
µm x 24 cm, coated
7. Before each sample application, perform the
following purge cycles:
60 seconds with deionized water
60 seconds with Washing Solution
60 seconds with deionized water
60 seconds
Micropure-EZ Enzyme Removers
200 µL) into the Micropure-EZ reservoir. Close cap.
2.Place assembled device in a microcentrifuge and spin at
full speed (12,000–14,000 × g) for 60 seconds. Spin time
will depend on the sample volume.
Data Sheet - P7957
Dilute a 3 µl aliquot of the C-24 Sequencing Primer
with 6 µl of 1X TE to make a final concentration of
1.67 pmol/µl.
2. Add 2-3 µl (3.5-5 pmoles) of C-24
Data Sheet - L6037
µl)
Medium in
wells (µl)
96-well 0.1-
0.5
5-25 95-75
24-well 0.5-2 25-100 225-150
6-well 2-6 100-300 900-700
60 mm 6-8 300-400 2200-2100
100 mm 8-12 400-600 4600-4400
GOAT ANTI-CHOLINE ACETYLTRANSFERASE (ChAT) AFFINITY PURIFIED POLYCLONAL ANTIBODY
reduced background add 1-5% BSA to diluent) for 60 minutes.
5. Rinse 3x3 minutes each with PBS.
6. Incubate with primary antisera diluted in diluent 12-24 hours (longer incubations may give better results
Data Sheet - P2188
µm x 24 cm, coated
7. Before each sample application, perform the
following purge cycles:
60 seconds with deionized water
60 seconds with Washing Solution
60 seconds with deionized water
60 seconds
Data Sheet - P2693
µm x 24 cm, coated
7. Before each sample application, perform the
following purge cycles:
60 seconds with deionized water
60 seconds with Washing Solution
60 seconds with deionized water
60 seconds
miR-24-3p Hu-Cy3 SmartFlare™ RNA Detection Probe
representative.
miR-24-3p Hu-Cy3
SmartFlare™
RNA Detection Probe
Cat. # SF-1093
pack size: 50µL (250 rxns)
Fast GC-A Practical Guide for Increasing Sample Throughput without Sacrificing Quality
Dichlorobenzene
16. 2-Methylphenol
17. Bis(2-chloroisopropyl)ether
18. N-Nitroso-di-n-propylamine
19. 4-Methylphenol
20. Hexachloroethane
21. Nitrobenzene-d5 (surr.)
22. Nitrobenzene
23. Isophorone
24. 2-Nitrophenol
Product Information Sheet - SHP001
pre-warmed
complete medium to cells and incubate for an
additional 24 hours
Day 5
Second harvest of Viral Particles
22. Between 60-72 hours post-transfection collect viral
particles by carefully
The 3D Petri Dish
-6EA 12-60TR 60 rods
24 well format24 well format
Z764043-6EA 24-96 96 small spheroids
Z764051-6EA 24-35 35 large spheroids
Z764108-6EA 24-1 spheroids, mixed pack
Z764078-6EA 24-24TR 24 rods
Z764086-
Preparation and Sequencing of Fosmid DNA Templates using the Montage BAC96 Miniprep Kit and Montage SEQ96 & SEQ384 Clean-up Kits and Plates
1. 95° C for 5 minutes
2. 95° C for 30 seconds
3. 50° C for 10 seconds2
4. 60° C for 4 minutes
5. Repeat steps 2-4 for a total of 100 cycles
6. Hold at 4 °C
Technical Data Sheet - Brix standards for Refractometry
shelf life is 24 month
Tested for homogeneity and stability
Storage at room temperature
° Brix
0°
5°
10°
20°
30°
40°
SureFlex Process Container Film
20 in. H2O/60 secs.
Testing performed at 25 °C
Oxygen transmission (O2) ASTM D-3985 0.28 cc/100 in.2 /24 hrs. (435 cc/m2/24 hrs.)
carbon Dioxide (cO2) ASTM D-1434 0.58 cc/100 in.2/24 hrs. (9.02
Product Information Sheet - PEPCE
µm x 24 cm, coated
7. Before each sample application, perform the
following purge cycles:
60 seconds with deionized water
60 seconds with Washing Solution
60 seconds with deionized water
60 seconds
Product Information Sheet - P2188
µm x 24 cm, coated
7. Before each sample application, perform the
following purge cycles:
60 seconds with deionized water
60 seconds with Washing Solution
60 seconds with deionized water
60 seconds
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