laboratory use only.
SPGPM10RJ
SPGPM20RJ
http://www.SigmaAldrich.com
PF07111w Rev 09/20 2 of 3
Directions to Use the Steripak™-GP
Filter with a Pressure Vessel
or Peristaltic
cycle essential for peptidoglycan
biosynthesis. J. Microbiol. Meth., 75, 70-74 (2008).
5. Makino, A. et al., Cinnamycin (Ro 09-0198)
promotes cell binding and toxicity by inducing
transbilayer
1 µL
Assay Buffer 75 µL
2. Add 80 µL of Working Reagent to each sample
well. Tap plate to mix.
Measurement
1. Incubate for 10 minutes at room temperature.
:
1. Combine:
600 µl of platelet-free human plasma
75 µl of 50 mM Tris-HCl, pH 7.4, with 100 mM NaCl
and 0.5% human serum albumin
75 µl of Gly-Pro-Arg-Pro solution (36 mg/ml,
Catalog Number
compounds, GW2974, inhibits
the proliferation of ErbB-2- and EGFR-expressing tumor
cell lines at concentrations 75-fold greater for tumor cells than for normal
cells. In
Reproducibility
Lane 1: Molecular
Weight Markers
Lanes 2–11: Replicates
Figure 2. Ten replicates of a human plasma sample (75 µL) were diluted with 125 µL
of equilibration buffer and individually
range is 75–300 g/ml.
As a growth supplement for use in monoclonal
antibody production the optimal range is
25–100 g/ml.
Storage/Stability
Store the product at 2–8 C. After reconstitution
to kg scale -75 to +190 °C
3 Kilo Labs: Safebridge®
Cat III capable
g to kg scale -75 to +190 °C
2 Safebridge® Cat IV
drying/packaging suites
g to kg scale -10 to +100
bar (75 psi)
5.2 bar (75 psi)
5.2 bar (75 psi)
Working temperature range 4–40 °C (39–104 °F)
Weight (without tubing) 127 g (4.5 oz)
Dimensions Manifold
Tubing
14.6 × 5.1 × 4.4 cm (5.75 ×2× 1.75
tear drop receiver plate. 2.
NOTE: The recommended volume is 150 µL per well.
Add cells at desired density to wells in the filter plate. 3.
NOTE: The recommended volume is 50-75 µL per well.
Gently
DNA was
measured.
Reference
1. Avanzi, G., et al., Br. J. Haematol., 69, 359 (1988).
KAA,PHC 09/06-1
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