CN
94-12-2
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关键词:'94-12-2'
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The Effective Use of Protein Kinase Inhibitors
ni
n
(W
1
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(1
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)
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(L
9
90
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(5
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)
Q
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Aryl acylamidase from Pseudomonas fluorescens ATCC 39005 (A4951)
7400 to 48 ml of
deionized water. PREPARE FRESH.)
ProductInformation
Revised: 12/21/94 Page 2 of 4
Enzymatic Assay of ARYL ACYLAMIDASE
(EC 3.5.1.13)
REAGENTS: (continued)
G. Color
The Effective Use of Protein Kinase Inhibitors
ni
n
(W
1
62
8)
(1
µ
M
)
LY
2
94
00
2
(L
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Q
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Data Sheet - C6236
-CALPAIN-94
(DOMAIN III, INSERT 2), LARGE SUBUNIT
Developed in Rabbit, Affinity Isolated Antibody
Product Number C 6236
Product Description
Anti-Calpain-94 (Domain III, Insert 2), Large Subunit
(P8289) - Enzyme Assay
Water 2.00
Reagent A (Buffer) 0.50
Reagent C (Mg(OAc)2) 0.25
Reagent D (AP) 0.10
Reagent E (DTT) 0.10
Reagent F (ATP) 0.05
Revised: 12/27/94 Page 3 of 4
Enzymatic Assay of PROTEIN KINASE CATALYTIC
Anti-Calpain-94 (Catalytic Domain) Large Subunit antibody produced in rabbit (C3239)
µ-calpain, m-calpain, nCL-2, nCL-3, etc). It binds to the
reduced and non-reduced protein. By immunoblotting
against the reduced protein, the antibody identifies
bands at 94 kDa, 82 kDa, 62 kDa, 60 kDa
Anti-Calpain-94 (Domain II) Large Submit antibody produced in rabbit (C3364)
antibody
recognizes latent and active calpain 94. It does not
crossreact with other calpain family members
(µ-calpain, m-calpain, nCL-2, nCL-3, etc). By immuno-
blotting against the reduced
Arginine kinase from lobster tail muscle (A3389)
1880.)
D. 2 M Potassium Chloride Solution (KCl)
(Prepare 10 ml in deionized water using Potassium Chloride, Sigma Prod. No. P-4504.)
ProductInformation
Revised: 09/12/94 Page 2 of 4
Enzymatic
Data Sheet - C6361
and
active protein. It binds to human calpain-94, but does
not crossreact with other calpain family members
(µ-calpain, M-calpain, LP-82/85 calpain, nCL-2, nCL-3,
etc.). The rat 88 kDa and 90 kDa calpains
Data Sheet - C6486
and active protein. It binds to
human calpain-94, but does not crossreact with other
calpain family members (µ-calpain, m-calpain, LP-82/85
calpain, nCL-2, nCL-3, etc). The rat 88 kDa and 90 kDa
forms
(L9656) - Enzyme Assay
Lipoprotein Lipase
in cold Reagent A.)
ProductInformation
SPPNPB01.001 Page 2 of 3
Revised: 01/12/94
Enzymatic Assay of LIPOPROTEIN LIPASE
(E.C. 3.1.1.34)
PROCEDURE:
Pipette (in milliliters
(L2254) - Enzyme Assay
Lipoprotein Lipase
in cold Reagent A.)
ProductInformation
SPPNPB01.001 Page 2 of 3
Revised: 01/12/94
Enzymatic Assay of LIPOPROTEIN LIPASE
(E.C. 3.1.1.34)
PROCEDURE:
Pipette (in milliliters
Data Sheet - C6611
and active proteins. It binds
to human calpain-94, but does not crossreact with other
calpain family members (µ-calpain, m-calpain, LP-82/85
calpain, nCL-2, nCL-3, etc.). The rat 88 kDa and 90
kDa calpains
Extract-N-Amp Tissue PCR Kit: Rapid Genomic DNA Extraction from Tissue Coupled with PCR
(Figure 2) 94 °C 3 min
25 ml Tissue Prep 35 cycles of 40 cycles of
0.5-1 cm of 100 ml Neut B 94 °C 45 sec 94 °C 30 sec
mouse tail tip 58 °C 1 min 58 °C
Anti-Calpain-94 (LP828588 90 Isoforms) (Domain I) Large Subunit antibody produced in rabbit (C3739)
m-calpain, nCL-2, nCL-3, etc). The antibody
binds to the reduced and non-reduced protein. By
immunoblotting against the reduced protein, the
antibody recognizes isoforms of calpain 94 (Lp82, 85,
88
(I9656) - Enzyme Assay
Lipoprotein Lipase
in cold Reagent A.)
ProductInformation
L9656 Page 2 of 3
SPPNPB01.001
Revised: 01/12/94
Enzymatic Assay of LIPOPROTEIN LIPASE
(E.C. 3.1.1.34)
Sigma Prod. Nos. L-2254
ANTI- PROMININ-1(CD133)
antigen.
The original paper is located at: http://www.pnas.org/cgi/reprint/94/23/12425.pd
IMMUNOGEN:
Telencephali of ten 12-day-old NMRI mouse embryos (E12, ~1 mg protein), which consist
mostly
(C5896) - Enzyme Assay
4504. Adjust to pH 8.0 at 37°C with 1 M HCl.)
ProductInformation
Revised: 03/17/94 Page 2 of 4
Enzymatic Assay of CHOLINE OXIDASE
(EC 1.1.3.17)
REAGENTS: (continued)
F.
(N5386) - Enzyme Assay
10 ml in deionized water using Perchloric Acid, Sigma Stock No. 24425-2.)
N5386 Page 2 of 3
SS-DNA03
Revised: 12/21/94
Enzymatic Assay of NUCLEASE, MICROCOCCAL
(EC 3.1.31.1)
PROCEDURE
(P1391) - Enzyme Assay
0.1 - 0.2 unit/ml of Alkaline
Phosphatase in cold Reagent C.)
P1391 Page 2 of 3
SPPNPP01
Revised: 07/12/94
Enzymatic Assay of PHOSPHATASE ALKALINE1
(EC 3.1.3.1 )
Glycine with Zinc Assay
(P4377) - Enzyme Assay
0.1 - 0.2 unit/ml of Alkaline
Phosphatase in cold Reagent C.)
P4377 Page 2 of 3
SPPNPP01
Revised: 07/12/94
Enzymatic Assay of PHOSPHATASE ALKALINE1
(EC 3.1.3.1 )
Glycine with Zinc Assay
Product Information - P2640
0.1 - 0.2 unit/ml of Alkaline
Phosphatase in cold Reagent C.)
P2640 Page 2 of 3
SPPNPP01
Revised: 07/12/94
Enzymatic Assay of PHOSPHATASE ALKALINE1
(EC 3.1.3.1 )
Glycine with Zinc Assay
(P5395) - Enzyme Assay
0.1 - 0.2 unit/ml of Alkaline
Phosphatase in cold Reagent C.)
P5395 Page 2 of 3
SPPNPP01
Revised: 07/12/94
Enzymatic Assay of PHOSPHATASE ALKALINE1
(EC 3.1.3.1 )
Glycine with Zinc Assay
(P8639) - Enzyme Assay
0.1 - 0.2 unit/ml of Alkaline
Phosphatase in cold Reagent C.)
P8639 Page 2 of 3
SPPNPP01
Revised: 07/12/94
Enzymatic Assay of PHOSPHATASE ALKALINE1
(EC 3.1.3.1 )
Glycine with Zinc Assay
(P4002) - Enzyme Assay
0.1 - 0.2 unit/ml of Alkaline
Phosphatase in cold Reagent C.)
P4002 Page 2 of 3
SPPNPP01
Revised: 07/12/94
Enzymatic Assay of PHOSPHATASE ALKALINE1
(EC 3.1.3.1 )
Glycine with Zinc Assay
(P5931) - Enzyme Assay
0.1 - 0.2 unit/ml of Alkaline
Phosphatase in cold Reagent C.)
P5931 Page 2 of 3
SPPNPP01
Revised: 07/12/94
Enzymatic Assay of PHOSPHATASE ALKALINE1
(EC 3.1.3.1 )
Glycine with Zinc Assay
Product Information - P3765
0.1 - 0.2 unit/ml of Alkaline
Phosphatase in cold Reagent C.)
P3765 Page 2 of 3
SPPNPP01
Revised: 07/12/94
Enzymatic Assay of PHOSPHATASE ALKALINE1
(EC 3.1.3.1 )
Glycine with Zinc Assay
Product Information - P7515
0.1 - 0.2 unit/ml of Alkaline
Phosphatase in cold Reagent C.)
P7515 Page 2 of 3
SPPNPP01
Revised: 07/12/94
Enzymatic Assay of PHOSPHATASE ALKALINE1
(EC 3.1.3.1 )
Glycine with Zinc Assay
(C4405) - Enzyme Assay
4504. Adjust to pH 8.0 at 37°C with 1 M HCl.)
ProductInformation
Revised: 03/17/94 Page 2 of 4
Enzymatic Assay of CHOLINE OXIDASE
(EC 1.1.3.17)
REAGENTS: (continued)
F.
(P4069) - Enzyme Assay
0.1 - 0.2 unit/ml of Alkaline
Phosphatase in cold Reagent C.)
P4069 Page 2 of 3
SPPNPP01
Revised: 07/12/94
Enzymatic Assay of PHOSPHATASE ALKALINE1
(EC 3.1.3.1 )
Glycine with Zinc Assay
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