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., Regulation of antioxidant metabolism
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., Characterization of a MAPKK-
like protein kinase TOPK. Biochem. Biophys. Res.
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2. Simons-Evelyn, M. et al., PBK/TOPK is a novel
mitotic kinase which is upregulated
., Characterization of a MAPKK-
like protein kinase TOPK. Biochem. Biophys. Res.
Commun., 325, 997-1004 (2004).
2. Simons-Evelyn, M. et al., PBK/TOPK is a novel
mitotic kinase which is upregulated
Lot Number 051M0821:
997 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7.2, 12.5 mM
glycerol 2-phosphate, 20 mM MgCl2, 25 mM MnCl2,
5 mM EGTA, and 2 mM EDTA
., Characterization of a MAPKK-
like protein kinase TOPK. Biochem. Biophys. Res.
Commun., 325, 997-1004 (2004).
2. Simons-Evelyn, M. et al., PBK/TOPK is a novel
mitotic kinase which is upregulated
., Characterization of a MAPKK-
like protein kinase TOPK. Biochem. Biophys. Res.
Commun., 325, 997-1004 (2004).
2. Simons-Evelyn, M. et al., PBK/TOPK is a novel
mitotic kinase which is upregulated
., Characterization of a MAPKK-
like protein kinase TOPK. Biochem. Biophys. Res.
Commun., 325, 997-1004 (2004).
2. Simons-Evelyn, M. et al., PBK/TOPK is a novel
mitotic kinase which is upregulated
Standard by
mixing 3 L of the 100 mM Glutathione
Standard with 997 L of purified water.
2. Prepare a 3 µM GSH Standard by mixing
5 L of the 300 µM GSH Standard with
495 L of
4. Samuelsson, B., et al., Thromboxanes
and thromboxanes. Annu. Rev.
Biochem., 47, 997-1029 (1978).
5. Long, C. R., et al., Thromboxane B2
induced changes in renal blood flow,
renal interstitial
1998) Death by a thousand cuts: an ever
increasing list of caspase substrates. Cell Death Differ. 5(12), 997-1000.
Homogeneous Caspases Assay, fluorimetric y Version 09
http://www.sigmaaldrich.com
duplicate wells.
1. Make a 3000 ng/ml stock solution by diluting 3 µl of the 1 mg/ml standard into 997 µl of 1X
ELISA Diluent. Then make a 92.3 ng/ml working stock solution by diluting 30.8 µl
4. Samuelsson, B., et al., Prostaglandins and
thromboxanes. Annu Rev Biochem; 47, 997-1029
(1978).
5. Long, C. R., et al., Prostaglandin E2 induced
changes in renal blood flow, renal interstitial
DNA
damage-induced G2-M checkpoint activation by histone H2AX and 53BP1. Nat Cell Biol.
2002;4:993-997. PMID: 12447390
12. Crowe SL, Movsesyan VA, Jorgensen TJ, Kondratyev A. Rapid phosphorylation of