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Nathan Nguyen et al.
The Journal of biological chemistry, 295(39), 13584-13600 (2020-08-01)
During autophagy, LC3 and GABARAP proteins become covalently attached to phosphatidylethanolamine on the growing autophagosome. This attachment is also reversible. Deconjugation (or delipidation) involves the proteolytic cleavage of an isopeptide bond between LC3 or GABARAP and the phosphatidylethanolamine headgroup. This
Mark S Lowenthal et al.
Analytical and bioanalytical chemistry, 407(18), 5453-5462 (2015-05-01)
Chromatographic separation of monosaccharides hydrolyzed from glycoconjugates or complex, aggregate biomaterials, can be achieved by classic analytical methods without a need for derivatizing the monosaccharide subunits. A simple and sensitive method is presented for characterizing underivatized monosaccharides following hydrolysis from
Yifeng Qian et al.
Lab on a chip, 19(7), 1267-1276 (2019-03-02)
To elucidate the secretary function of immune cells, we develop a nanoplasmonic circular interferometric biosensor based on intensity interrogation for label-free and dynamic sensing of molecular secretion. Exceptional sensitivity has been demonstrated through coupling free light and surface plasmon polariton
Immunization of broiler chickens with five newly identified surface-exposed proteins unique to Clostridium perfringens causing necrotic enteritis.
Heidarpanah, et al.
Scientific Reports, 13, 5254-5254 (2023)
Christian B Brøchner et al.
Glia, 64(1), 90-104 (2015-08-22)
The glycosphingolipid SSEA-4 and the glycoprotein YKL-40 have both been associated with human embryonic and neural stem cell differentiation. We investigated the distribution of SSEA-4 and YKL-40 positive cells in proliferative zones of human fetal forebrain using immunohistochemistry and double-labeling
K Benschop et al.
Journal of clinical microbiology, 46(12), 3965-3970 (2008-10-24)
Human parechoviruses (HPeV) are widespread pathogens belonging to the Picornavirus family. Six genotypes, which have predominantly been isolated from children, are known. Data on prevalence of HPeV genotypes are generally based on cell culture, which may underestimate the prevalence of
A S Chaudhry
Journal of animal physiology and animal nutrition, 89(11-12), 403-412 (2006-01-13)
Two experiments compared the suitability of two commercial enzymes to estimate in vitro proteolysis of different proteins. Experiment 1 compared the proteolytic activity over various incubation times of a microbial enzyme (protease from Streptomyces griseus) with a plant enzyme (papain
Ajay Kumar Yagati et al.
Bioelectrochemistry (Amsterdam, Netherlands), 128, 165-174 (2019-04-21)
Most clinical tests for biomarker detection require the support of a laboratory, and the results are usually slow, less sensitive, and lack the possibility for Point-of-Care (PoC) testing. Further, with the increasing demand for sensitive, portable, rapid, and low-cost devices
Michelle Quintero-García et al.
Nutrients, 12(5) (2020-05-21)
Osteoporosis is a disease of the skeletal system characterized by low bone mass and bone weakening, which increase the risk of fracture. This disease is associated with menopause because hypoestrogenism induces the maturation and activation of osteoclasts. In addition, a
W Qi et al.
Journal of dairy science, 90(11), 5004-5011 (2007-10-24)
Bovine casein was digested with pepsin at pH 2.0 in a batch-stirred tank reactor. To investigate the effect of peptic digestion on the aggregate size and molecular weight distribution of bovine casein, the resulting hydrolysates were examined by size-exclusion chromatography
Hongxiu Liu et al.
Microbial pathogenesis, 142, 104055-104055 (2020-02-15)
Serine protease is the virulence factor of many pathogens. However, there are no prevailing data available for serine protease as a virulence factor derived from Mycobacterium avium subsp. paratuberculosis (MAP). The MAP3292c gene from MAP, the predicted serine protease, was
Ezequiel Hernández-Becerra et al.
Biological trace element research, 197(1), 224-232 (2020-01-11)
This study evaluates the effect of three calcium levels in the diet (normal, moderate, and severe calcium depletion) on bone metabolism of male Wistar rats during their growth period. Bone mineral density (BMD) and femur length were determined in vivo
C Poelaert et al.
Animal : an international journal of animal bioscience, 12(6), 1154-1164 (2017-11-03)
Over the past decade, in vitro methods have been developed to study intestinal fermentation in pigs and its influence on the digestive physiology and health. In these methods, ingredients are fermented by a bacterial inoculum diluted in a mineral buffer
Danyel Bueno Dalto et al.
Journal of animal science, 97(9), 3938-3946 (2019-07-12)
The present study compared the net intestinal absorption of zinc (Zn) and copper (Cu) after meals containing different dietary ratios among these trace elements. Ten 46-kg pigs were used in a cross-over design to assess the 10-h net portal-drained viscera
Danyel Bueno Dalto et al.
Journal of animal science, 98(3) (2020-02-23)
The present study compares the net portal appearance of dietary iron (Fe) and selenium (Se) after meals containing different sources and levels of these minerals. Twelve pigs (55.1 ± 3.7 kg) were used in a cross-over design to assess the
Juan Carlos Cordova et al.
Methods in molecular biology (Clifton, N.J.), 1486, 317-341 (2016-11-16)
Energy-dependent protein degradation is studied through the dual bead ClpXP motility assay. Processing of folded proteins involves recognition, unfolding, translocation, and degradation stages. A dual optical trap, in a passive force-clamp geometry, exhibits bead-to-bead displacements that directly follow subprocesses underlying
Junko Kasuya et al.
Journal of neurogenetics, 33(3), 164-178 (2019-05-18)
The Drosophila mutant paraShu harbors a dominant, gain-of-function allele of the voltage-gated sodium channel gene, paralytic (para). The mutant flies display severe seizure-like phenotypes, including neuronal hyperexcitability, spontaneous spasms, ether-induced leg shaking, and heat-induced convulsions. We unexpectedly found that two
Xinwang Cao et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(50), 21121-21125 (2009-11-27)
The Golgi-associated four-phosphate adaptor protein 2 (FAPP2) has been shown to possess transfer activity for glucosylceramide both in vitro and in cells. We have previously shown that FAPP2 is involved in apical transport from the Golgi complex in epithelial MDCK
Lindsey J Gray et al.
Fly, 15(1), 47-59 (2021-03-12)
Evolved metabolic thriftiness in humans is a proposed contributor to the obesity epidemic. Insect models have been shown to evolve both 'metabolic thrift' in response to rearing on high-protein diets that promote leanness, and 'obesity resistance' when reared on fattening
Chungwon Park et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(9) (2021-02-25)
Maintaining the balance between neuronal excitation and inhibition is essential for proper function of the central nervous system. Inhibitory synaptic transmission plays an important role in maintaining this balance. Although inhibitory transmission has higher kinetic demands compared to excitatory transmission
Betsaida Bibo-Verdugo et al.
Marine biotechnology (New York, N.Y.), 17(4), 416-427 (2015-04-17)
A chymotrypsin was purified from the gastric juice of California spiny lobster (Panulirus interrutpus), using preparative electrophoresis and affinity chromatography on agarose-p-aminobenzamidine. The molecular mass was estimated by polyacrylamide gel electrophoresis (SDS-PAGE) under denaturing conditions to be 28 kDa. Chymotrypsin
S Jake Gonzales et al.
Frontiers in immunology, 13, 809264-809264 (2022-06-21)
Memory B cells (MBCs) and plasma antibodies against Plasmodium falciparum (Pf) merozoite antigens are important components of the protective immune response against malaria. To gain understanding of how responses against Pf develop in these two arms of the humoral immune
Tianyang Liu et al.
Nature communications, 13(1), 6988-6988 (2022-11-18)
Plasmodium species cause malaria and kill hundreds of thousands annually. The microtubule-based motor kinesin-8B is required for development of the flagellated Plasmodium male gamete, and its absence completely blocks parasite transmission. To understand the molecular basis of kinesin-8B's essential role
L M Kotzé-Hörstmann et al.
Food & function, 13(24), 12648-12663 (2022-11-29)
Current pharmaceutical treatments addressing obesity are plagued by high costs, low efficacy and adverse side effects. Natural extracts are popular alternatives, but evidence for their anti-obesity properties is scant. We assessed the efficacy of a green (minimally-oxidized) Rooibos (Aspalathus linearis)
Fatemeh Kiaeian Moosavi et al.
Archives of insect biochemistry and physiology, 103(4), e21655-e21655 (2020-01-19)
Some studies have highlighted benefits for Lobesia botrana by adding Botrytis cinerea mycelium to an artificial larval diet and have suggested a mutualistic relationship between the two organisms on grapevine, hypothesizing that fungal sterols were the nutritional factor involved. Because
Regina L Agulto et al.
eLife, 10 (2021-07-27)
The microtubule-associated protein, doublecortin-like kinase 1 (DCLK1), is highly expressed in a range of cancers and is a prominent therapeutic target for kinase inhibitors. The physiological roles of DCLK1 kinase activity and how it is regulated remain elusive. Here, we
Kevin R Sieber et al.
Caries research, 53(4), 457-466 (2019-05-16)
Casein and mucin have been shown to improve the erosion-protective properties of the pellicle when applied in combination. The aim of this in vitro study was to optimize the concentrations of these 2 proteins to achieve a maximum protective effect.
Arthur H Cheng et al.
Cell reports, 26(12), 3191-3202 (2019-03-21)
Clock neurons within the mammalian suprachiasmatic nuclei (SCN) encode circadian time using interlocked transcription-translation feedback loops (TTFLs) that drive rhythmic gene expression. However, the contributions of other transcription factors outside of the circadian TTFLs to the functionality of the SCN
Tom Flossmann et al.
Cell reports, 26(12), 3173-3182 (2019-03-21)
Synchronized activity is a universal characteristic of immature neural circuits that is essential for their developmental refinement and strongly depends on GABAergic neurotransmission. A major subpopulation of GABA-releasing interneurons (INs) expresses somatostatin (SOM) and proved critical for rhythm generation in
Sreya Biswas et al.
Nature communications, 13(1), 2995-2995 (2022-06-01)
Hepatitis B virus has infected a third of the world's population, and 296 million people are living with chronic infection. Chronic infection leads to progressive liver disease, including hepatocellular carcinoma and liver failure, and there remains no reliable curative therapy.
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