Advanced gene editing

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facet applications:Advanced gene editing

facet content type:Technical Article

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Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

Cas9-Mediated Recombineering for Kdo2-Lipid A

E. coli CRISPR/Cas-mediated Lambda-Red HR vector system enables gene editing via homology-directed repair.

CRISPR Essentials: MISSION® CRISPR gRNAs, Cas9 and Related Products

Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments.

Long Oligos

Techniques for long oligo synthesis and purification offer superior quality unmatched by other suppliers.

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

Safe Lentivirus Handling

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

MISSION® Lentiviral Controls

When conducting shRNA experiments, proper controls are a key element of experimental design. Proper use of controls permits accurate interpretation of knockdown results and provides assurance of the specificity of the observed phenotype. We offer a variety of positive, negative

shRNA Gene Families Sets

MISSION® shRNA Library gene sets mapped by Bioinformatics team for specific cellular pathways.

Small Molecule CRISPR Enhancers

Modulation of homology-directed repair (HDR) within the context of CRISPR-genome editing has led to the identification of small molecules that enhance CRISPR-mediated HDR efficiency in various cell types.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

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