Gel electrophoresis

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Bis Tris Polyacrylamide Gel Electrophoresis Technology

Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.

Components of Rehydration Solution

This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Reproducibility with Biological Buffers

Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

Troubleshooting 2-D DIGE Results

Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.

DNA / Protein Electrophoresis and Troubleshooting Tables

Precipitation Procedures

This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

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