Protein expression

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facet applications:Protein expression

facet content type:Technical Article

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Tag Removal Proteases for Recombinant Protein Purification

Proteases for biotinylated tag removal for protein purification workflows with related reagents and technical resources.

Cell-Free Protein Synthesis or Expression

A form of synthetic biology, cell-free protein synthesis or expression (CFPE) is a rapid method to produce recombinant proteins in solution without involving cells. Using biomolecular translation machinery extracted from a range of different cell types, researchers can express a

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Simplicon™ in vivo Protein Expression System

Simplicon: A non-genome integrating, tunable and sustained protein expression system in human cells, based on a single, polycistronic, and self-replicating synthetic RNA.

Troubleshooting Guide for Duolink® PLA for Flow Cytometry Detection

General tips and tricks for proper experiment execution, aid in identifying potential problems, and provide solutions to ensure a successful Duolink® PLA experiment for flow cytometry.

c-Src kinase (Csk)

The C-terminal c-Src kinase (Csk) is a 50-kDa cytosolic tyrosine kinase expressed in all examined cell types

FLAG® Tag Bacterial and Mammalian Expression Vectors

FLAG® and 3xFLAG® expression vectors and products for bacterial and mammalian expression vectors, detection and purification of proteins.

Caspases Inhibitors and Activators

Agents reported to activate cellular caspases include chemotherapeutic drugs, TNF receptor agonists, and other enzymes. Inhibitors of apoptosis were the first identified endogenous caspase inhibitors.

Phospholipase A2

Phospholipase A2 (PLA2) designates a class of enzymes that hydrolyze the sn-2 ester of glycerophospholipids to produce a fatty acid and a lysophospholipid. It has become clear that some of these enzymes liberate arachidonic acid in mammalian cells for the

Chloride Channels

We offer many products related to chloride channels for your research needs.

Adenosine Receptors

We offers many products related to adenosine receptors for your research needs.

Calcium Channels

We offers many products related to calcium channels for your research needs.

MAPKKKs

The extracellular signal regulated kinase (ERK1 and ERK2) pathways are activated by mitogens and play an important role in controlling cell growth and differentiation.

Prostanoid Receptors

We offer many products related to prostanoid receptors for your research needs.

Cannabinoid Receptors

We offer many products related to cannabinoid receptors for your research needs.

α2-Adrenoceptors

Learn about alpha-2 adrenoceptor and its subtypes, mediated responses, and applications of agonists. Included is a list of available products and a comparison table.

Serotonin Receptors

We offer many products related to serotonin receptors for your research needs.

GABAC Receptors

Introduction to GABAC receptors as well as modulators and other products for your research needs.

Serotonin Synthesis and Metabolism

Serotonin (5-hydroxytryptamine) is principally found stored in three main cell types - i) serotonergic neurons in the CNS and in the intestinal myenteric plexus, ii) enterochromaffin cells in the mucosa of the gastrointestinal tract, and iii) in blood platelets. Metabolism

Histamine Receptors

We offers many products related to histamine receptors for your research needs.

Sigma Receptors

Sigma receptors may constitute favorable targets for drug design in conditions such as psychiatric and movement disorders, amnesia, depression, cancer, inflammation and cocaine addiction.

Nuclear Receptors (Non-Steroids)

We offer many products related to non-steroid nuclear receptors for your research needs.

Cytokine Receptors (Hematopoetin Receptor Family)

We offer many products related to Cytokine Receptors (Hematopoetin Receptor Family) for your research needs.

Dopamine Receptors

We offer many products related to dopamine receptors for your research needs.

EGFR

Our company offers many products related to EGFR for your research needs.

Dopamine and Norepinephrine Metabolism

Either monoamine oxidase A (MAO) or catechol-O-methyltransferase (COMT) can catalyze the first step in catecholamine catabolism. The potentially toxic aldehyde intermediate generated in the MAO reaction (3,4-dihydroxyphenylacetaldehyde for dopamine, 3,4-dihydroxyphenylglycolaldehyde for norepinephrine) is either rapidly reduced to an alcohol (by

FGFR

We offer many products related to FGFR for your research needs.

Canonical Wnt / β-Catenin Signaling

We review the Wnt signaling pathway (also, canonical Wnt or beta (β)-catenin pathway) and provide links to related products at sigmaaldrich.com.

Residual Peroxide Determination in Food Packaging

ECM microenvironment shapes cell processes; engineering advances needed for complex control in cell and tissue studies.

Phosphoprotein Phosphatases (Tyrosine)

Protein tyrosine phosphatases' catalytic mechanism involves transient phosphorylation.

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