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Protein purification
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facet applications:Protein purification
facet content type:Technical Article
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ANTI-FLAG® M2磁珠
FLAG®表达系统是一种表达、纯化和检测重组融合蛋白的成熟方法。作为FLAG®的可靠供应商，Sigma®现提供FLAG标签抗体标记的免疫磁珠，用于免疫沉淀、蛋白纯化和蛋白互作研究。ANTI-FLAG® M2磁珠由ANTI-FLAG® M2鼠单克隆抗体与超顺磁铁包覆的4%琼脂糖微珠偶联而成，平均粒径50 µM。M2抗体可结合哺乳动物、细菌和植物提取物融合蛋白N端、Met-N端或C端的FLAG®肽。
PureProteom 自动化 IgG 纯化
通过 PureProteome™ Protein A 和 Protein G 磁珠，可利用 KingFisher Duo 磁珠纯化仪快速、可重现地纯化免疫球蛋白 (IgG)。
使用Amicon® Pro离心过滤器进行蛋白质标记和纯化
市售蛋白标记试剂盒让研究人员可以灵活定制自己的免疫检测panel。
细胞器分离
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
抗体亲和色谱的脱盐和缓冲液置换
实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
酵母细胞裂解试剂
我们的酵母细胞裂解试剂具有通用性、无磷酸盐、非变性等特点。CelLytic Y细胞裂解试剂可作为酵母细胞裂解和蛋白溶解的有效方法。CelLytic Y酵母酶解试剂盒为从酿酒酵母、巴斯德毕赤酵母和粟酒裂殖酵母酵母菌株中高效形成原生质球和提取蛋白提供了一种便捷的方法。
Anti-FLAG® M2磁珠
比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。
Amicon® Pro 纯化系统 – 蛋白纯化
Amicon Pro 让蛋白纯化更简单。此系统可提供一致的样品制备，可实现更可靠的蛋白回收率、同时保证纯度、简化数据生成。
色谱柱填充和抗体亲和层析的制备
本页介绍了高效的色谱柱填充和抗体亲和层析的制备。
The Potential of Dendritic Polyester Scaffolds as Biocompatible Drug Delivery Agents
Professor Michael Malkoch provides an overview of the unique properties of dendritic molecules and their application in biomedical fields. He highlights bis-MPA dendritic scaffolds as a promising biodegradable and biocompatible platform for drug delivery applications
Manual and Automated Purification
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
Anti-FLAG® M2 Magnetic Beads
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
Chelators
BioUltra Chelators. A chelate is a water-soluble complex between a metal ion and a complexing agent. It usually does not dissociate easily in solution, but forms an inert complex.
Converting from Flow Velocity to Volumetric Flow Rates
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
Yeast Cell Lysis Reagents
Our yeast cell lysis reagents are versatile, phosphate-free, and non-denaturing. CelLytic Y Cell Lysis reagent provides an effective method for yeast cell lysis and protein solubilization. The CelLytic Y Yeast Enzymatic Lysis Kit provides a convenient method for
Organelle Isolation
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify
Agarose Beads Vs. Magnetic Beads in ChIP
Agarose beads Vs. Magnetic beads in Chromatin Immunoprecipitation (ChIP)
Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification
Troubleshooting of Cleavage Methods
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
General Considerations for Purification of GST-tagged Protein
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
HIC as a Capture Step
This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.
Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography
Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature
Performing an Isolation of Recombinant Protein Complexes
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.
Troubleshooting Purification Methods
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
Troubleshooting Guide for Affinity Chromatography of Tagged Proteins
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Performing High-Throughput Screening Using His MultiTrap™ HP and His MultiTrap™ FF 96-Well filter Plates
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
Optimizing Purification of Histidine-Tagged Proteins
How to optimize purification of histidine-tagged proteins using Cytiva products.
Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON® Columns
His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.
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