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G5635
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Boyuan Song et al.
Ultramicroscopy, 203, 145-154 (2019-02-11)
Direct electron detectors are an essential asset for the resolution revolution in electron cryo microscopy of biological objects. The direct detectors provide two modes of data acquisition; the counting mode in which single electrons are counted, and the integrating mode
M H Hetta et al.
Die Pharmazie, 69(2), 92-95 (2014-03-20)
The aim of this work was the estimation of resveratrol content in two successive extracts (EtOAc and MeOH) of peanuts (Arachis hypogaea L.) pericarp of Egypt, by TLC and HPLC methods. Results showed the presence of 3.0 and 0.5 microg/mL
Anca D Petrescu et al.
Protein expression and purification, 58(2), 184-193 (2008-01-08)
Acyl coenzyme A binding protein (ACBP) has been proposed to transport fatty acyl CoAs intracellularly, facilitating their metabolism. In this study, a new mouse recombinant ACBP was produced by insertion of a histidine (his) tag at the C-terminus to allow
Reciprocal regulation of pH 6 antigen gene loci by PhoP and RovA in Yersinia pestis biovar Microtus.
Yiquan Zhang et al.
Future microbiology, 8(2), 271-280 (2013-02-05)
To explore the transcriptional regulation of the psaEF and psaABC loci by the RovA and PhoP regulators in Yersinia pestis. Primer extension, LacZ fusion, gel mobility shift and DNase I footprinting assays were conducted in combination for this gene regulation
Weixing Dai et al.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 29(3), 303-309 (2013-05-07)
To establish the yeast-based bioassay system for the androgenic endocrine disruptors in the environment. The recombinant gene-transformed yeast cells were constructed based on two episomal vectors. In the expression vector, the expression of androgen receptor (AR) gene was driven by
E Paschke et al.
Human genetics, 109(2), 159-166 (2001-08-21)
An inherited deficiency in beta-galactosidase can result in GM1 gangliosidosis, with several phenotypes of generalized or chronic psychomotor deterioration, as well as in Morquio disease type B, a characteristic mucopolysaccharidosis free of neurological symptoms. We performed mutation analyses in 17
Yu Qian et al.
Development (Cambridge, England), 140(8), 1751-1761 (2013-03-28)
Peritubular myoid cells (PMCs) are myofibroblast-like cells that surround the seminiferous tubules and play essential roles in male fertility. How these cells modulate spermatogenesis and the signaling pathways that are involved are largely unknown. Here we report that Lgr4 is
Yasir Hasan Siddique et al.
Journal of insect science (Online), 12, 92-92 (2013-02-23)
In the present study the effect of 0.1, 0.2, 0.4, 0.8, and 1.0 µL/mL of the steroid K-canrenoate was evaluated in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg(9) for 6, 24, and 48 hours of duration. The
Urs Lewandrowski et al.
Molecular & cellular proteomics : MCP, 5(2), 226-233 (2005-11-03)
Among known platelet proteins, a prominent and functionally important group is represented by glycoprotein isoforms. They account e.g. for secretory proteins and plasma membrane receptors including integrins and glycoprotein VI as well as intracellular components of cytosol and organelles including
Han Liu et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(46), 18555-18560 (2013-10-30)
The tongue is a critical element of the feeding system in tetrapod animals for their successful adaptation to terrestrial life. Whereas the oral part of the mammalian tongue contains soft tissues only, the avian tongue has an internal skeleton extending
Assays for bacterial mucin-desulfating sulfatases.
A M Roberton et al.
Methods in molecular biology (Clifton, N.J.), 125, 417-426 (2000-05-23)
Alexandra Moores et al.
Journal of bacteriology, 196(1), 148-156 (2013-10-29)
The phase variation (reversible on-off switching) of the type 1 fimbrial adhesin of Escherichia coli involves a DNA inversion catalyzed by FimB (switching in either direction) or FimE (on-to-off switching). Here, we demonstrate that RfaH activates expression of a FimB-LacZ
Kathryn K Bercury et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(13), 4466-4480 (2014-03-29)
During CNS development, oligodendrocytes, the myelinating glia of the CNS, progress through multiple transitory stages before terminating into fully mature cells. Oligodendrocyte differentiation and myelination is a tightly regulated process requiring extracellular signals to converge to elicit specific translational and
Juan Antonio Marchal et al.
Drug design, development and therapy, 7, 1301-1313 (2013-11-07)
Bozepinib [(RS)-2,6-dichloro-9-[1-(p-nitrobenzenesulfonyl)-1,2,3,5-tetrahydro-4,1-benzoxazepin-3-yl]-9H-purine] is a potent antitumor compound that is able to induce apoptosis in breast cancer cells. In the present study, we show that bozepinib also has antitumor activity in colon cancer cells, showing 50% inhibitory concentration (IC50) values lower
Radhika Dixit et al.
Development (Cambridge, England), 140(21), 4398-4406 (2013-10-17)
Recent studies have shown that mesothelial progenitors contribute to mesenchymal lineages of developing organs. To what extent the overlying mesothelium contributes to lung development remains unknown. To rigorously address this question, we employed Wt1(CreERT2/+) mice for high-fidelity lineage tracing after
Edgar Rodríguez-Negrete et al.
Methods in molecular biology (Clifton, N.J.), 1072, 241-258 (2013-10-19)
The yeast two-hybrid system is currently one of the most standardized protein interaction mapping techniques. The rationale of the yeast two-hybrid system relies on the physical separation of the DNA-binding domain from the transcriptional activation domain of several transcription factors.
J W Callahan
Biochimica et biophysica acta, 1455(2-3), 85-103 (1999-11-26)
GM1 gangliosidosis and Morquio B disease are distinct disorders both clinically and biochemically yet they arise from the same beta-galactosidase enzyme deficiency. On the other hand, galactosialidosis and sialidosis share common clinical and biochemical features, yet they arise from two
Purification and properties of a beta-hexosidase from Sporobolomyces singularis.
J A Blakely et al.
Canadian journal of biochemistry, 47(11), 1021-1025 (1969-11-01)
Alan Merk et al.
IUCrJ, 7(Pt 4), 639-643 (2020-07-23)
We report the determination of the structure of Escherichia coli β-galactosidase at a resolution of ∼1.8 Å using data collected on a 200 kV CRYO ARM microscope equipped with a K3 direct electron detector. The data were collected in a single 24 h
A Hinek et al.
The Journal of clinical investigation, 91(3), 1198-1205 (1993-03-01)
We and others have previously shown that a 67-kD cell surface elastin/laminin-binding protein (EBP) is responsible for cell adhesion to elastin and laminin and for mediating the process of elastin fiber assembly, but the nature of this protein was unknown.
Javier A Menendez et al.
Cell cycle (Georgetown, Tex.), 12(4), 555-578 (2013-02-02)
Aging can be viewed as a quasi-programmed phenomenon driven by the overactivation of the nutrient-sensing mTOR gerogene. mTOR-driven aging can be triggered or accelerated by a decline or loss of responsiveness to activation of the energy-sensing protein AMPK, a critical
N Ishii et al.
Clinical genetics, 48(2), 103-108 (1995-08-01)
Morquio B disease was found in a 15-year-old Japanese boy who presented with progressive generalized skeletal dysplasia without neurological manifestations. Mild keratan sulfaturia was found, and beta-galactosidase was deficient in fibroblasts. Gene analysis revealed two mutant alleles, 83Tyr-->His (Y83H) and
Daniela S Gerhard et al.
Genome research, 14(10B), 2121-2127 (2004-10-19)
The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random
S Privitera et al.
The Journal of biological chemistry, 273(11), 6319-6326 (1998-04-16)
Our previous studies showed immunological and functional similarities, as well as partial sequence homology, between the enzymatically inactive alternatively spliced variant of human beta-galactosidase (S-gal) and the 67-kDa elastin/laminin-binding protein (EBP) from sheep. To define the genetic origin of the
Toshiya Suzuki et al.
Plant physiology, 173(1), 183-205 (2016-11-12)
Pollen exine is essential for protection from the environment of the male gametes of seed-producing plants, but its assembly and composition remain poorly understood. We previously characterized Arabidopsis (Arabidopsis thaliana) mutants with abnormal pollen exine structure and morphology that we
Arun Azad et al.
International journal of radiation oncology, biology, physics, 88(2), 385-394 (2014-01-15)
To examine the effects of combined blockade of DNA-dependent protein kinase (DNA-PK) and poly(adenosine diphosphate-ribose) polymerase-1 (PARP-1) on accelerated senescence in irradiated H460 and A549 non-small cell lung cancer cells. The effects of KU5788 and AG014699 (inhibitors of DNA-PK and
Emmanuel Roze et al.
Movement disorders : official journal of the Movement Disorder Society, 20(10), 1366-1369 (2005-06-30)
GM1 gangliosidosis is due to beta-galactosidase deficiency. Only patients with type 3 disease survive into adulthood and develop movement disorders. Clinical descriptions of this form are rare, particularly in non-Japanese patients. We describe four new patients and systematically analyze all
Jennifer M Stratford et al.
PloS one, 9(9), e107238-e107238 (2014-09-06)
Fos-Tau-LacZ (FTL) transgenic mice are used to visualize the anatomical connectivity of neurons that express c-Fos, an immediate early gene, in response to activation. In contrast to typical c-Fos protein expression, which is localized to the nucleus of stimulated neurons
Zachary C Ryan et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(15), 6199-6204 (2013-03-27)
Inactivating mutations of the SOST (sclerostin) gene are associated with overgrowth and sclerosis of the skeleton. To determine mechanisms by which increased amounts of calcium and phosphorus are accreted to enable enhanced bone mineralization in the absence of sclerostin, we
N G Asp et al.
The Biochemical journal, 114(2), 351-359 (1969-09-01)
1. Two beta-galactosidases from human small-intestinal mucosa were separated by gel-filtration chromatography and the properties of the two enzymes were studied. Lactose and four hetero beta-galactosides were used as substrates. 2. One of the enzymes was particle-bound and could be
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