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J D Shoemaker et al.
Journal of chromatography, 562(1-2), 125-138 (1991-01-02)
Eighty-five clinical urine samples and nineteen urine samples previously found by other laboratories to suggest genetic metabolic defects were prepared for trimethylsilylation by treatment with urease, followed by azeotropic dehydration. The "Target Analyte Search" program provided with the VG Trio
Minsang Lee et al.
The Plant journal : for cell and molecular biology, 41(5), 685-696 (2005-02-11)
Homoserine kinase (HSK) produces O-phospho-l-homoserine (HserP) used by cystathionine gamma-synthase (CGS) for Met synthesis and threonine synthase (TS) for Thr synthesis. The effects of overexpressing Arabidopsis thaliana HSK, CGS, and Escherichia coli TS (eTS), each controlled by the 35S promoter
S M Nayeemul Bari et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(24), 9926-9931 (2013-05-30)
Cholera epidemics have long been known to spread through water contaminated with human fecal material containing the toxigenic bacterium Vibrio cholerae. However, detection of V. cholerae in water is complicated by the existence of a dormant state in which the
Ce Feng Liu et al.
Biochemistry, 52(9), 1603-1610 (2013-02-08)
Autoinducer inactivator A (AiiA) is a metal-dependent N-acyl homoserine lactone hydrolase that displays broad substrate specificity but shows a preference for substrates with long N-acyl substitutions. Previously, crystal structures of AiiA in complex with the ring-opened product N-hexanoyl-l-homoserine revealed binding
K G Gazarian et al.
Medical hypotheses, 58(4), 279-283 (2002-05-25)
The hepatitis B infection leads to various profound pathological processes in liver metabolism. Some biochemical alterations detectable by blood analysis are currently used for a preliminary evaluation of the infection. Based on existing data we present here evidence that non-protein
Chunjuan Miao et al.
Biochemical and biophysical research communications, 427(2), 293-298 (2012-09-22)
N-acyl-homoserine lactones (AHLs) are a class of bacterial quorum-sensing (QS) signals that are commonly used by Gram-negative bacteria for cell-to-cell communication. Recently, it has become evident that AHLs can regulate plant root growth and trigger plant defense responses; however, little
Magdalena Górny et al.
Molecules (Basel, Switzerland), 24(23) (2019-11-27)
Impaired glutathione (GSH) synthesis and dopaminergic transmission are important factors in the pathophysiology of schizophrenia. Our research aimed to assess the effects of l-buthionine-(S,R)-sulfoximine (BSO), a GSH synthesis inhibitor, and GBR 12909, a dopamine reuptake inhibitor, administered alone or in
Edinaldo Rogério da Silva Moraes et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 907, 1-6 (2012-09-22)
The present study describes a simple and efficient method utilizing high performance liquid chromatography (HPLC) coupled to fluorescence detection for the determination of kinetic parameters of glutamate uptake in nervous tissue. Retinal tissue obtained from 7-day-old chicks was incubated with
Sun-Pyo Kim et al.
Current microbiology, 65(6), 701-710 (2012-09-11)
This study was undertaken to determine whether cyclic AMP (cAMP) or cAMP-receptor protein (CRP) modulates the activity of the autoinducer (AI)-2-mediated quorum sensing (QS) system in response to glucose availability in Vibrio vulnificus. A mutation in crp impaired V. vulnificus
Sandra N Majuta et al.
Journal of the American Society for Mass Spectrometry, 30(6), 1102-1114 (2019-04-14)
Rapid, solution-phase hydrogen/deuterium exchange (HDX) coupled with mass spectrometry (MS) is demonstrated as a means for distinguishing small-molecule metabolites. HDX is achieved using capillary vibrating sharp-edge spray ionization (cVSSI) to allow sufficient time for reagent mixing and exchange in micrometer-sized
Amanda L Garner et al.
Chemical communications (Cambridge, England), 49(15), 1515-1517 (2013-01-19)
As a guide for chemical probe design, focused analogue synthetic studies were undertaken upon the lactone ring of 3-oxo-C(12)-homoserine lactone. We have concluded that hydrolytic instability of the heterocyclic ring is pivotal for its ability to modulate immune signaling and
Ryoko Taguchi et al.
American journal of physiology. Cell physiology, 307(2), C162-C168 (2014-05-23)
N-acyl-homoserine lactones (AHL) are quorum-sensing molecules in bacteria that play important roles in regulating virulence gene expression in pathogens such as Pseudomonas aeruginosa. The present study compared responses between undifferentiated and differentiated Caco-2 cells to N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL). A low
Michal Brunwasser-Meirom et al.
Nature communications, 7, 10407-10407 (2016-02-03)
We explore a model for 'quenching-like' repression by studying synthetic bacterial enhancers, each characterized by a different binding site architecture. To do so, we take a three-pronged approach: first, we compute the probability that a protein-bound dsDNA molecule will loop.
Jing Yu et al.
Infection and immunity, 81(11), 4053-4062 (2013-08-21)
The autoinducer-2 (AI-2) quorum-sensing system has been linked to diverse phenotypes and regulatory changes in pathogenic bacteria. In the present study, we performed a molecular and biochemical characterization of the AI-2 system in Yersinia pestis, the causative agent of plague.
Dongsheng Luo et al.
Journal of agricultural and food chemistry, 67(37), 10401-10411 (2019-08-24)
This research applied inhibitors to reduce the content of cooked off-flavor components (dimethyl sulfide, dimethyl disulfide, dimethyl trisulfide, and 3-(methylthio)propanaldehyde) in heat-treated melon juice. The effects of glucose oxidase (GOD) on the formation and release of these four volatile sulfur
Dan Yu et al.
BMC microbiology, 12, 288-288 (2012-12-12)
Staphylococcus aureus is an important pathogen that causes biofilm-associated infection in humans. Autoinducer 2 (AI-2), a quorum-sensing (QS) signal for interspecies communication, has a wide range of regulatory functions in both Gram-positive and Gram-negative bacteria, but its exact role in
Kuk-Ki Hong et al.
Journal of industrial microbiology & biotechnology, 41(10), 1517-1524 (2014-08-27)
There has been a significant global interest to produce bulk chemicals from renewable resources using engineered microorganisms. Large research programs have been launched by academia and industry towards this goal. Particularly, C4 chemicals such as succinic acid (SA) and 1,4-butanediol
Hsuan-Chen Wu et al.
Molecular systems biology, 9, 636-636 (2013-01-24)
Escherichia coli were genetically modified to enable programmed motility, sensing, and actuation based on the density of features on nearby surfaces. Then, based on calculated feature density, these cells expressed marker proteins to indicate phenotypic response. Specifically, site-specific synthesis of
Danuta Kowalczyk-Pachel et al.
PloS one, 11(1), e0147238-e0147238 (2016-01-26)
The role of cocaine in modulating the metabolism of sulfur-containing compounds in the peripheral tissues is poorly understood. In the present study we addressed the question about the effects of acute and repeated (5 days) cocaine (10 mg/kg i.p.) administration
Walter M Blair et al.
Journal of microbiological methods, 92(3), 250-252 (2013-01-12)
The Vibrio harveyi autoinducer-2 (AI-2) bioassay is used routinely to screen for inhibition of the AI-2 quorum sensing system. The present study utilizes three well-described bacterial strains to demonstrate that inconsistent normalization across matrices undermines the assay's use in screening
D C Turnell et al.
Clinical chemistry, 28(3), 527-531 (1982-03-01)
This method for estimating clinically important amino acids in serum or urine within 40 min involves o-phthalaldehyde/2-mercaptoethanol derivatization and reversed-phase "high-pressure" liquid chromatography. Homocysteic acid is an internal standard, and homoserine and norvaline are reference peaks. For all the amino
Harshad Lade et al.
Journal of microbiology and biotechnology, 25(11), 1908-1919 (2015-07-04)
This work investigated the potential of curcumin (CCM) and (-)-epigallocatechin gallate (EGCG) to inhibit N-acyl homoserine lactone (AHL)-mediated biofilm formation in gramnegative bacteria from membrane bioreactor (MBR) activated sludge. The minimum inhibitory concentrations (MICs) of CCM alone against all the
Varnika Roy et al.
Applied microbiology and biotechnology, 97(6), 2627-2638 (2012-10-12)
Quorum sensing (QS), the process of autoinducer-mediated cell-cell signaling among bacteria, facilitates biofilm formation, virulence, and many other multicellular phenotypes. QS inhibitors are being investigated as antimicrobials because of their potential to reduce symptoms of infectious disease while slowing the
Vorrapon Chaikeeratisak et al.
Cell, 177(7), 1771-1780 (2019-06-15)
Cargo trafficking along microtubules is exploited by eukaryotic viruses, but no such examples have been reported in bacteria. Several large Pseudomonas phages assemble a dynamic, tubulin-based (PhuZ) spindle that centers replicating phage DNA sequestered within a nucleus-like structure. Here, we
Anneli Nordqvist et al.
Bioorganic & medicinal chemistry, 16(10), 5501-5513 (2008-05-09)
A combination of a literature survey, structure-based virtual screening and synthesis of a small library was performed to identify hits to the potential antimycobacterial drug target, glutamine synthetase. The best inhibitor identified from the literature survey was (2S,5R)-2,6-diamino-5-hydroxyhexanoic acid (4
S Ravanel et al.
Archives of biochemistry and biophysics, 316(1), 572-584 (1995-01-10)
Cystathionine gamma-synthase, the first enzyme specific for the methionine biosynthetic pathway, was purified to apparent homogeneity from spinach leaf chloroplasts. A nonradioactive assay based on O-phthaldialdehyde derivatization of L-cystathionine and fluorescence detection was developed to determine the cystathionine gamma-synthase activity.
H Ozaki et al.
Journal of biochemistry, 91(4), 1163-1171 (1982-04-01)
Out of 27 strains of methionine auxotrophs of Brevibacterium flavum, 14 strains did not grow on homoserine but grew on O-acetylhomoserine, and all were found to lack homoserine O-acetyltransferase [EC 2.3.1.31] alone. Another 3 strains did not grow on O-acetylhomoserine
Xiangan Han et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 52(9), 1167-1172 (2012-12-15)
The LuxS/AI-2 quorum sensing (QS) system shared by Gram-positive and Gram-negative bacteria involves the production of autoinducer-2 (AI-2). In this study, the method of biosynthesis of AI-2 was established using recombinant LuxS and Pfs of avian pathogenic Escherichia coli (APEC)
Magdalena Skoczylas et al.
Journal of chromatography. A, 1609, 460514-460514 (2019-09-29)
Quantitative structure - retention relationships analysis was applied to investigate the molecular retention mechanism of proteinogenic and non-proteinogenic amino acids on the amino acid- and peptide-silica stationary phases. Twelve stationary phases with chemically bonded amino acids of different types (glycine
Minhao Wu et al.
The Journal of biological chemistry, 288(22), 15878-15887 (2013-04-17)
Quorum-sensing systems are widely used by bacteria to control behavior in response to fluctuations in cell density. Several small diffusible molecules called autoinducers act as signaling molecules in quorum-sensing processes through interplay with sensors. Autoinducers modulate vital physiological functions such
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