N9877

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Detailed study of the interaction between human herpesvirus 6B glycoprotein complex and its cellular receptor, human CD134.

Huamin Tang et al.

Journal of virology, 88(18), 10875-10882 (2014-07-11)

Recently, we identified a novel receptor, CD134, which interacts with the human herpesvirus 6B (HHV-6B) glycoprotein (g)H/gL/gQ1/gQ2 complex and plays a key role in the entry of HHV-6B into target cells. However, details of the interaction between the HHV-6B gH/gL/gQ1/gQ2

Synergistic effect of muramyl dipeptide with heat shock protein 70 from Mycobacterium tuberculosis on immune activation.

Tae-Hyoun Kim et al.

Immunobiology, 220(1), 26-31 (2014-12-03)

Heat shock protein 70 from Mycobacterium tuberculosis (Mtb Hsp70) has been known to modulate immune response including dendritic cell activation. Muramyl dipeptide (MDP) is an immunoreactive derivative of peptidoglycan from all Gram-negative and Gram-positive bacteria and recognized to be responsible

Binding of calmodulin changes the calcineurin regulatory region to a less dynamic conformation.

Cuiping Fu et al.

International journal of biological macromolecules, 79, 235-239 (2015-05-10)

Calcineurin (CN) is a Ca(2+)/calmodulin (CaM) activated serine/threonine phosphatase, and its regulatory region (CNRR) plays a critical role in the coupling of Ca(2+) signals to cellular responses. Ca(2+)/CaM binds to the CNRR, resulting in a conformational change that removes an

Biophysical Characterization of a Vaccine Candidate against HIV-1: The Transmembrane and Membrane Proximal Domains of HIV-1 gp41 as a Maltose Binding Protein Fusion.

Zhen Gong et al.

PloS one, 10(8), e0136507-e0136507 (2015-08-22)

The membrane proximal region (MPR, residues 649-683) and transmembrane domain (TMD, residues 684-705) of the gp41 subunit of HIV-1's envelope protein are highly conserved and are important in viral mucosal transmission, virus attachment and membrane fusion with target cells. Several

Ion conductivity of the bacterial translocation channel SecYEG engaged in translocation.

Denis G Knyazev et al.

The Journal of biological chemistry, 289(35), 24611-24616 (2014-07-13)

While engaged in protein transport, the bacterial translocon SecYEG must maintain the membrane barrier to small ions. The preservation of the proton motif force was attributed to (i) cation exclusion, (ii) engulfment of the nascent chain by the hydrophobic pore

PUB22 and PUB23 U-BOX E3 ligases directly ubiquitinate RPN6, a 26S proteasome lid subunit, for subsequent degradation in Arabidopsis thaliana.

Seok Keun Cho et al.

Biochemical and biophysical research communications, 464(4), 994-999 (2015-07-21)

Drought stress strongly affects plant growth and development, directly connected with crop yields, accordingly. However, related to the function of U-BOX E3 ligases, the underlying molecular mechanisms of desiccation stress response in plants are still largely unknown. Here we report

Novel cathepsin B and cathepsin B-like cysteine protease of Naegleria fowleri excretory-secretory proteins and their biochemical properties.

Jinyoung Lee et al.

Parasitology research, 113(8), 2765-2776 (2014-05-17)

Naegleria fowleri causes a lethal primary amoebic meningoencephalitis (PAM) in humans and experimental animals, which leads to death within 7-14 days. Cysteine proteases of parasites play key roles in nutrient uptake, excystment/encystment, host tissue invasion, and immune evasion. In this

Crystal structure of the 5hmC specific endonuclease PvuRts1I.

Asgar Abbas Kazrani et al.

Nucleic acids research, 42(9), 5929-5936 (2014-03-19)

PvuRts1I is a prototype for a larger family of restriction endonucleases that cleave DNA containing 5-hydroxymethylcytosine (5hmC) or 5-glucosylhydroxymethylcytosine (5ghmC), but not 5-methylcytosine (5mC) or cytosine. Here, we report a crystal structure of the enzyme at 2.35 Å resolution. Although

Slow binding kinetics of secreted protein, acidic, rich in cysteine-VEGF interaction limit VEGF activation of VEGF receptor 2 and attenuate angiogenesis.

Marzena Cydzik et al.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 29(8), 3493-3505 (2015-04-30)

VEGF-A (VEGF) drives angiogenesis through activation of downstream effectors to promote endothelial cell proliferation and migration. Although VEGF binds both VEGF receptor 1 (R1) and receptor 2 (R2), its proangiogenic effects are attributed to R2. Secreted protein, acidic, rich in

Enterococcus faecalis 6-phosphogluconolactonase is required for both commensal and pathogenic interactions with Manduca sexta.

Jonathan F Holt et al.

Infection and immunity, 83(1), 396-404 (2014-11-12)

Enterococcus faecalis is a commensal and pathogen of humans and insects. In Manduca sexta, E. faecalis is an infrequent member of the commensal gut community, but its translocation to the hemocoel results in a commensal-to-pathogen switch. To investigate E. faecalis

Olfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure.

Matti F Pronker et al.

The Journal of biological chemistry, 290(24), 15092-15101 (2015-04-24)

Olfactomedin-1 (Olfm1; also known as noelin and pancortin) is a member of the olfactomedin domain-containing superfamily and a highly expressed neuronal glycoprotein important for nervous system development. It binds a number of secreted proteins and cell surface-bound receptors to induce

Interleukin-6 and lipopolysaccharide modulate hepcidin mRNA expression by HepG2 cells.

Pia Villarroel et al.

Biological trace element research, 150(1-3), 496-501 (2012-10-16)

Iron homeostasis is controlled by hepcidin (Hpc) as well as other ways. Hpc expression is regulated by iron (Fe) storage and by inflammation, but the joint effect of both stimuli remains unclear. We studied the modulatory role of inflammatory agents

A Disulfide Bond-forming Machine Is Linked to the Sortase-mediated Pilus Assembly Pathway in the Gram-positive Bacterium Actinomyces oris.

Melissa E Reardon-Robinson et al.

The Journal of biological chemistry, 290(35), 21393-21405 (2015-07-15)

Export of cell surface pilins in Gram-positive bacteria likely occurs by the translocation of unfolded precursor polypeptides; however, how the unfolded pilins gain their native conformation is presently unknown. Here, we present physiological studies to demonstrate that the FimA pilin

Molecular and biochemical characterization of Paragonimus westermani tyrosinase.

Y-A Bae et al.

Parasitology, 142(6), 807-815 (2015-01-27)

Trematode tyrosinases (TYRs) play a major role in the tanning process during eggshell formation. We investigated the molecular and biochemical features of Paragonimus westermani TYR (PwTYR). The PwTYR cDNA was composed of 1568-bp encompassing a 1422-bp-long open reading frame (474-amino

Effects of actin-like proteins encoded by two Bacillus pumilus phages on unstable lysogeny, revealed by genomic analysis.

Yihui Yuan et al.

Applied and environmental microbiology, 81(1), 339-350 (2014-10-26)

We characterized two newly isolated myoviruses, Bp8p-C and Bp8p-T, infecting the ginger rhizome rot disease pathogen Bacillus pumilus GR8. The plaque of Bp8p-T exhibited a clear center with a turbid rim, suggesting that Bp8p-T could transform into latent phage. Lysogeny

TYPE-ONE PROTEIN PHOSPHATASE4 regulates pavement cell interdigitation by modulating PIN-FORMED1 polarity and trafficking in Arabidopsis.

Xiaola Guo et al.

Plant physiology, 167(3), 1058-1075 (2015-01-07)

In plants, cell morphogenesis is dependent on intercellular auxin accumulation. The polar subcellular localization of the PIN-FORMED (PIN) protein is crucial for this process. Previous studies have shown that the protein kinase PINOID (PID) and protein phosphatase6-type phosphatase holoenzyme regulate

Chloroplast SRP54 Was Recruited for Posttranslational Protein Transport via Complex Formation with Chloroplast SRP43 during Land Plant Evolution.

Beatrix Dünschede et al.

The Journal of biological chemistry, 290(21), 13104-13114 (2015-04-03)

In bacteria, membrane proteins are targeted cotranslationally via a signal recognition particle (SRP). During the evolution of higher plant chloroplasts from cyanobacteria, the SRP pathway underwent striking adaptations that enable the posttranslational transport of the abundant light-harvesting chlorophyll-a/b-binding proteins (LHCPs).

CsCCL17, a CC chemokine of Cynoglossus semilaevis, induces leukocyte trafficking and promotes immune defense against viral infection.

Yong-Hua Hu et al.

Fish & shellfish immunology, 45(2), 771-779 (2015-06-09)

CC chemokines are the largest subfamily of chemokines, which are important components of the innate immune system. To date, sequences of several CC chemokines have been identified in half-smooth tongue sole (Cynoglossus semilaevis); however, the activities and functions of these

Structure-based Design Targeted at LOX-1, a Receptor for Oxidized Low-Density Lipoprotein.

Shraddha Thakkar et al.

Scientific reports, 5, 16740-16740 (2015-11-19)

Atherosclerosis related cardiovascular diseases continue to be the primary cause of mortality in developed countries. The elevated level of low density lipoprotein (LDL) is generally considered to be the driver of atherosclerosis, but recent years have seen a shift in

Combining free and aggregated cellulolytic systems in the cellulosome-producing bacterium Ruminiclostridium cellulolyticum.

Julie Ravachol et al.

Biotechnology for biofuels, 8, 114-114 (2015-08-14)

Ruminiclostridium cellulolyticum and Lachnoclostridium phytofermentans (formerly known as Clostridium cellulolyticum and Clostridium phytofermentans, respectively) are anaerobic bacteria that developed different strategies to depolymerize the cellulose and the related plant cell wall polysaccharides. Thus, R. cellulolyticum produces large extracellular multi-enzyme complexes

A highly responsive and selective fluorescent probe for imaging physiological hydrogen sulfide.

Zhi-jie Chen et al.

Biochemistry, 53(37), 5966-5974 (2014-08-21)

The discovery of hydrogen sulfide (H2S) as a novel gasotransmitter for cell signaling and other pathophysiological processes has spurred tremendous interest in developing analytical methods for its detection in biological systems. Herein, we report the development of a highly responsive

Continuous colorimetric screening assay for detection of d-amino acid aminotransferase mutants displaying altered substrate specificity.

Janet E B Barber et al.

Analytical biochemistry, 463, 23-30 (2014-06-21)

D-Amino acid aminotransferase (DAAT) catalyzes the synthesis of numerous d-amino acids, making it an attractive biocatalyst for the production of enantiopure d-amino acids. To bolster its biocatalytic applicability, improved variants displaying increased activity toward non-native substrates are desired. Here, we

Nitrilotriacetic acid and its salts.

IARC monographs on the evaluation of carcinogenic risks to humans, 48, 181-212 (1990-01-01)

CodY Regulates SigD Levels and Activity by Binding to Three Sites in the fla/che Operon.

Qutaiba O Ababneh et al.

Journal of bacteriology, 197(18), 2999-3006 (2015-07-15)

Exponentially growing cultures of Bacillus subtilis (PY79) are composed primarily of nonmotile, chained cells. The alternative sigma factor, SigD, promotes the phenotypic switch from nonmotile, chained cells to unchained, motile cells. In the present work, we investigated the role of

Biochemical and cellular evidence demonstrating AKT-1 as a binding partner for resveratrol targeting protein NQO2.

Tze-chen Hsieh et al.

PloS one, 9(6), e101070-e101070 (2014-06-27)

AKT plays an important role in the control of cell proliferation and survival. Aberrant activation of AKT frequently occurs in human cancers making it an attractive drug targets and leading to the synthesis of numerous AKT inhibitors as therapeutic candidates.

Recombinant protein truncation strategy for inducing bactericidal antibodies to the macrophage infectivity potentiator protein of Neisseria meningitidis and circumventing potential cross-reactivity with human FK506-binding proteins.

Magdalena K Bielecka et al.

Infection and immunity, 83(2), 730-742 (2014-12-03)

A recombinant macrophage infectivity potentiator (rMIP) protein of Neisseria meningitidis induces significant serum bactericidal antibody production in mice and is a candidate meningococcal vaccine antigen. However, bioinformatics analysis of MIP showed some amino acid sequence similarity to human FK506-binding proteins

Receptor usage and cell entry of bat coronavirus HKU4 provide insight into bat-to-human transmission of MERS coronavirus.

Yang Yang et al.

Proceedings of the National Academy of Sciences of the United States of America, 111(34), 12516-12521 (2014-08-13)

Middle East respiratory syndrome coronavirus (MERS-CoV) currently spreads in humans and causes ∼ 36% fatality in infected patients. Believed to have originated from bats, MERS-CoV is genetically related to bat coronaviruses HKU4 and HKU5. To understand how bat coronaviruses transmit

Salvage of the thiamin pyrimidine moiety by plant TenA proteins lacking an active-site cysteine.

Rémi Zallot et al.

The Biochemical journal, 463(1), 145-155 (2014-07-12)

The TenA protein family occurs in prokaryotes, plants and fungi; it has two subfamilies, one (TenA_C) having an active-site cysteine, the other (TenA_E) not. TenA_C proteins participate in thiamin salvage by hydrolysing the thiamin breakdown product amino-HMP (4-amino-5-aminomethyl-2-methylpyrimidine) to HMP

In-gel expression and in situ immobilization of proteins for generation of three dimensional protein arrays in a hydrogel matrix.

Ju-Young Byun et al.

Lab on a chip, 13(5), 886-891 (2013-01-11)

A method has been developed for the direct conversion of DNA arrays into three dimensional protein arrays on a hydrogel matrix. An agarose gel embedded with bacterial protein synthesis machinery was used as the DNA-programmable expression gel matrix for the

The p.R1819_C1948delinsS mutation makes von Willebrand factor ADAMTS13-resistant and reduces its collagen-binding capacity.

Viviana Daidone et al.

British journal of haematology, 170(4), 564-573 (2015-04-24)

This report concerns abnormal ADAMTS13 (a disintegrin and metalloprotease with a thrombospondin type 1 motif, member 13) and collagen interactions coinciding with the p.R1819_C1948delinsS von Willebrand factor (VWF) mutation associated with the deletion of the C-terminus of the A3 domain

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