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Philippe Pierrat et al.
Langmuir : the ACS journal of surfaces and colloids, 31(45), 12362-12371 (2015-10-29)
Understanding the ionization behavior of lipid membranes is a key parameter for successful development of lipid-based drug delivery systems. Accurate determination of the ionization state of a titratable species incorporated in a lipid bilayer however requires special care. Herein we
Marco M Domingues et al.
International journal of molecular sciences, 22(5) (2021-04-04)
Cholesterol is responsible for the plasticity of plasma membranes and is involved in physiological and pathophysiological responses. Cholesterol homeostasis is regulated by oxysterols, such as 25-hydroxycholesterol. The presence of 25-hydroxycholesterol at the membrane level has been shown to interfere with
Jilong Li et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 26(9), 2178-2188 (2018-05-29)
Lyophilized keratinocyte-targeted nanocarriers (TLNκ) loaded with locked nucleic acid (LNA) modified anti-miR were developed for topical application to full thickness burn injury. TLNκ were designed to selectively deliver LNA-anti-miR-107 to keratinocytes using the peptide sequence ASKAIQVFLLAG. TLNκ employed DOTAP/DODAP combination
Saša Vrhovec Hartman et al.
New biotechnology, 47, 60-66 (2018-03-05)
Microcavities provide a well-controlled flow-free microenvironment and play an important role in many microfluidic systems, for example as cell-culturing microchambers. Here we show that transient concentration gradients that emerge during diffusive exchange of solutes in microcavities induce passive migration (diffusiophoresis)
Léa P Wilhelm et al.
The EMBO journal, 36(10), 1412-1433 (2017-04-06)
StAR-related lipid transfer domain-3 (STARD3) is a sterol-binding protein that creates endoplasmic reticulum (ER)-endosome contact sites. How this protein, at the crossroad between sterol uptake and synthesis pathways, impacts the intracellular distribution of this lipid was ill-defined. Here, by using
Jürgen Kreiter et al.
Biomolecules, 10(5) (2020-05-06)
Several mitochondrial proteins, such as adenine nucleotide translocase (ANT), aspartate/glutamate carrier, dicarboxylate carrier, and uncoupling proteins 2 and 3, are suggested to have dual transport functions. While the transport of charge (protons and anions) is characterized by an alteration in
Saori Nonaka et al.
Frontiers in immunology, 11, 520-520 (2020-04-16)
Pseudomonas entomophila is a highly pathogenic bacterium that infects insects. It is also used as a suitable model pathogen to analyze Drosophila's innate immunity. P. entomophila's virulence is largely derived from Monalysin, a β-barrel pore-forming toxin that damages Drosophila tissues
Michael J Munson et al.
Communications biology, 4(1), 211-211 (2021-02-18)
RNA-based therapies have great potential to treat many undruggable human diseases. However, their efficacy, in particular for mRNA, remains hampered by poor cellular delivery and limited endosomal escape. Development and optimisation of delivery vectors, such as lipid nanoparticles (LNPs), are
Karolina Sapoń et al.
Membranes, 10(8) (2020-08-06)
We developed a sensitive fluorescence-based assay for determination of exosome concentration. In our assay, Cholera toxin subunit B (CTB) conjugated to a fluorescence probe and a gel filtration technique (size-exclusion chromatography) are used. Exosomal membranes are particularly enriched in raft-forming
Xue Li et al.
Frontiers in bioengineering and biotechnology, 8, 1027-1027 (2020-10-13)
Hybrid porous nanoscale metal organic frameworks (nanoMOFs) made of iron trimesate are attracting increasing interest as drug carriers, due to their high drug loading capacity, biodegradability, and biocompatibility. NanoMOF surface modification to prevent clearance by the innate immune system remains
Younes F Barooji et al.
Scientific reports, 6, 30054-30054 (2016-07-23)
Membrane deformation is a necessary step in a number of cellular processes such as filopodia and invadopodia formation and has been shown to involve membrane shaping proteins containing membrane binding domains from the IRSp53-MIM protein family. In reconstituted membranes the
Peng Guo et al.
Theranostics, 6(1), 1-13 (2016-01-02)
Lipocalin 2 (Lcn2) is a promising therapeutic target as well as a potential diagnostic biomarker for breast cancer. It has been previously shown to promote breast cancer progression by inducing the epithelial to mesenchymal transition in breast cancer cells as
Juan L Paris et al.
Molecules (Basel, Switzerland), 25(14) (2020-07-28)
Complexes combining nucleic acids with lipids and polymers (lipopolyplexes) show great promise for gene therapy since they enable compositional, physical and functional versatility to be optimized for therapeutic efficiency. When developing lipopolyplexes for gene delivery, one of the first evaluations
Zuolong Chen et al.
Journal of immunology (Baltimore, Md. : 1950), 200(9), 3117-3127 (2018-03-28)
Invariant NKT (iNKT) cells have been shown to help B cells in a cognate or noncognate manner; however, whether cognate iNKT cell help induces B cell memory responses remains controversial, and the underlying mechanisms are still unclear. In this study
D Belletti et al.
International journal of pharmaceutics, 490(1-2), 85-93 (2015-05-20)
In this investigation, Nutlin-3 (Nut3), a novel antitumor drug with low water solubility (<0.1mg/L at 25°C), was loaded into liposomes (Lipo-Nut3), polymeric nanoparticles (NPs-Nut3) and nanoparticles engineered with an antibody direct against Syndecan-1/CD 138 (Syn-NPs-Nut3) to obtain carriers targeted to
Peter Bieling et al.
The EMBO journal, 37(1), 102-121 (2017-11-17)
WASP-family proteins are known to promote assembly of branched actin networks by stimulating the filament-nucleating activity of the Arp2/3 complex. Here, we show that WASP-family proteins also function as polymerases that accelerate elongation of uncapped actin filaments. When clustered on
Matthew J Sydor et al.
Biochimica et biophysica acta. Biomembranes, 1862(9), 183313-183313 (2020-04-19)
Engineered nano-materials (ENM) have been reported to affect lipid membrane permeability in cell models, but a mechanistic understanding of how these materials interact with biological membranes has not been described. To assess mechanisms of permeability, liposomes composed of DOPC, DOPS
A B Gomide et al.
Biochimica et biophysica acta, 1828(5), 1384-1389 (2013-02-05)
Using phase contrast and fluorescence microscopy we study the influence of the alkylphospholipid, ALP, 10-(octyloxy) decyl-2-(trimethylammonium) ethyl phosphate, ODPC, in giant unilamellar vesicles, GUVs, composed of DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), brain sphingomyelin (SM) and cholesterol (Chol). The results show that adding 100μM
Elena Lantero et al.
Pharmaceutics, 12(9) (2020-09-03)
Heparin is a promising antimalarial drug due to its activity in inhibiting Plasmodium invasion of red blood cells and to the lack of resistance evolution by the parasite against it, but its potent anticoagulant activity is preventing the advance of
Allison Faig et al.
Langmuir : the ACS journal of surfaces and colloids, 31(43), 11875-11885 (2015-10-22)
Cationic amphiphiles have received increasing attention as antimicrobials given their unique ability to disrupt bacteria cell membranes. While extensive research has demonstrated that amphiphiles' hydrophobic-to-charge ratio significantly modulates antibacterial activity, less work has focused on elucidating the specific impact of
S Malekkhaiat Häffner et al.
Physical chemistry chemical physics : PCCP, 19(35), 23832-23842 (2017-07-07)
Membrane interactions are critical for the successful use of inorganic nanoparticles as antimicrobial agents and as carriers of, or co-actives with, antimicrobial peptides (AMPs). In order to contribute to an increased understanding of these, we here investigate effects of particle
Po-Yu Peng et al.
Langmuir : the ACS journal of surfaces and colloids, 31(13), 3904-3911 (2015-03-10)
Forming fluid supported lipid bilayers (SLBs) on a gold surface can enable various lipid-membrane-associated biomolecular interactions to be investigated by several surface sensing techniques, such as surface plasmon resonance and scanning tunneling microscopy. However, forming fluid SLBs on a gold
Emilio J González-Ramírez et al.
Scientific reports, 10(1), 14085-14085 (2020-08-26)
The biophysical properties of sphingolipids containing lignoceric (C24:0) or nervonic (C24:1) fatty acyl residues have been studied in multicomponent lipid bilayers containing cholesterol (Chol), by means of confocal microscopy, differential scanning calorimetry and atomic force microscopy. Lipid membranes composed of
Jonathan P Litz et al.
Biophysical journal, 110(3), 635-645 (2016-02-04)
Recent results provide evidence that cholesterol is highly accessible for removal from both cell and model membranes above a threshold concentration that varies with membrane composition. Here we measured the rate at which methyl-β-cyclodextrin depletes cholesterol from a supported lipid
Sandrine L Verstraeten et al.
Scientific reports, 9(1), 7285-7285 (2019-05-16)
The membrane activity of some saponins, such as digitonin or alpha-hederin, is usually attributed to their interaction with membrane cholesterol (Chol). This contrasts with our recent publication showing that Chol, contrary to sphingomyelin (SM), can delay the cytotoxicity of the
Naoya Matsuo et al.
Journal of lipid research, 60(7), 1199-1211 (2019-05-16)
In mammals, lipids are selectively transported to specific sites using multiple classes of lipoproteins. However, in Drosophila, a single class of lipoproteins, lipophorin, carries more than 95% of the lipids in the hemolymph. Although a unique ability of the insect
Lucie Kocourková et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 170, 247-255 (2016-07-28)
Antimicrobial peptides have long been considered as promising compounds against drug-resistant pathogens. In this work, we studied the secondary structure of antimicrobial peptides melectin and antapin using electronic (ECD) and vibrational circular dichroism (VCD) spectroscopies that are sensitive to peptide
Youngjun Woo et al.
Journal of biomedical nanotechnology, 9(4), 610-614 (2013-04-30)
We have developed hydrodynamic filtration method in microfluidic device for the efficient size-selection of polydisperse lipid vesicles for giant unilamellar vesicles (GUVs), in which vesicles were formed by electroformation method. Combining pinched flow channel design before hydrodynamic filtration, GUVs were
Bruno F B Silva et al.
Biochimica et biophysica acta, 1838(1 Pt B), 398-412 (2013-09-26)
Cationic liposome-DNA (CL-DNA) complexes, are regarded as promising materials for safe and efficient delivery of genes for therapeutical applications. In order to be used in vivo, these complexes may be coated with a hydrophilic polymer (e.g. polyethylene-glycol, PEG) that provides
Shrikrishnan Sankaran et al.
Small (Weinheim an der Bergstrasse, Germany), 11(46), 6187-6196 (2015-10-16)
In this work, the development of a photoresponsive platform for the presentation of bioactive ligands to study receptor-ligand interactions has been described. For this purpose, supramolecular host-guest chemistry and supported lipid bilayers (SLBs) have been combined in a microfluidic device.
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