Pcr thermocycler

PCR Protocols and Guides - Simplify your gene discovery

fidelity thermophilic DNA polymerases. Materials and Methods Thermocycler DNA amplification was performed on MJ Research PTC-200 thermocyclers that had recently been calibrated by the manufacturer. Reactions

Data Sheet - A7721

to prevent evaporation if not using a thermocycler with a heated lid. 4. The amplification parameters will vary depending on the primers and the thermocycler used. It may be necessary to optimize

Amplifluor® SNPs HT Genotyping System for FAM-JOE

reagents in a PCR-designated hood in a template-free PCR room (recommended). 2. Program thermocycler with conditions described in Table 7. Start the program and pause the thermocycler at 96°C to preheat

Data Sheet - P9217

will vary depending on the primers and the thermocycler used. It may be necessary to optimize the system for individual primers, template, and thermocycler. Common cycling parameters are: a. Denature

Product Information Sheet - P8092

will vary depending on the primers and the thermocycler used. It may be necessary to optimize the system for individual primers, template, and thermocycler. Common cycling parameters are: a. Denature

Product Information Sheet - OPT2

10X PCR buffers for evaluation. 2. Layer 25 µl of mineral oil over the reaction mix in every tube if using a thermocycler without a heated lid. 3. Place the tubes in the thermocycler

Data Sheet - P8842

will vary depending on the primers and the thermocycler used. It may be necessary to optimize the system for individual primers, template, and thermocycler. Common cycling parameters are: a. Denature

Amplifluor® SNPs HT Genotyping System for Assay Development

reagents in a PCR-designated hood in a template-free PCR room (recommended). 2. Program thermocycler with conditions described in Table 7. Start the program and pause the thermocycler at 96°C to preheat

Amplifluor® SNPs HT Genotyping System for FAM-SR

reagents in a PCR-designated hood in a template-free PCR room (recommended). 2. Program thermocycler with conditions described in Table 7. Start the program and pause the thermocycler at 96°C to preheat

Product Information Sheet - P8467

will vary depending on the primers and the thermocycler used. It may be necessary to optimize the system for individual primers, template, and thermocycler. Common cycling parameters are: a. Denature

Product Information Sheet - T4809

Mineral Oil, Product Code M 8662 (optional) • 0.5 ml or 0.2 ml thin-walled PCR tubes, Product Codes P 3114 and P 3364 • Thermocycler • Primers • DNA to be amplified • Chloroform, Product Code C 7559 (

Product Information Sheet - 11636138001

, pH 7.0 (e. g., PCR Nucleotide Mix*) • Water, PCR Grade*.  Depending on the type of thermocycler, the usage of mineral is optional. Mineral oil must be used in case a thermocycler without heating lid

Product Information Sheet - D7442

polymerase for reliable PCR amplification, DNA contaminants can be introduced into PCR through a number of other reagents.2 To further minimize the risk of contaminant DNA during PCR, we include 10× MTP

Product Information Sheet-11888412001

be omitted if you are using a PCR cycler that does not require an oil overlay, according to the recommendations of the manufacturer. Place the sample in a thermocycler and start an appropriate cycling

Product Information Sheet - WGA3

Vortex thoroughly, centrifuge briefly, and begin thermocycling. The following profile has been optimized for a PE 9700 or equivalent thermocycler: Initial Denaturation 95 C for 3 minutes

Product Information Sheet-2gfrmkb

time. Recommendations for hold times are based on the ramp rate of the thermocycler, as well as the yield required. Thermocyclers with ramp speeds higher than 3°C/sec are considered fast, while slow cyclers

User Guide

Little or no PCR product is detected. Extract was not neutralized Make sure SRE0087, Neutralization Solution for Blood was added to the extract before PCR was performed. PCR reaction is

User Guide

Little or no PCR product is detected. Extract was not neutralized Make sure SRE0087, Neutralization Solution for Blood was added to the extract before PCR was performed. PCR reaction is

Product Information Sheet-exppcrkb

performed in a standard thermocycler, heating block, or waterbath, after which the sample is centrifuged and the DNA-containing supernatant recovered. Extracts may be used directly in PCR, without quantification

Product Information Sheet-expextkb

performed in a standard thermocycler, heating block, or waterbath, after which the sample is centrifuged and the DNA-containing supernatant recovered. Extracts may be used directly in PCR, without quantification

T7E1001 - Technical Bulletin

centrifuging. 3. Transfer the tubes to a PCR machine and begin thermocycling using the following conditions as defined in Table 2. Table 2. Thermocycle Program for PCR Reactions Cycle Step Temp Time

Product Information Sheet-SRE0086

Little or no PCR product is detected. Extract was not neutralized Make sure Cat. No. SRE0087, Neutralization Solution for Blood was added to the extract before PCR was performed. PCR reaction

Product Information Sheet-SRE0087

Little or no PCR product is detected. Extract was not neutralized Make sure Cat. No. SRE0087, Neutralization Solution for Blood was added to the extract before PCR was performed. PCR reaction

Product Information Sheet-2gfhsrmkb

time. Recommendations for hold times are based on the ramp rate of the thermocycler, as well as the yield required. Thermocyclers with ramp speeds higher than 3°C/sec are considered fast, while slow cyclers

Product Information Sheet-2gfhsrmdkb

time. Recommendations for hold times are based on the ramp rate of the thermocycler, as well as the yield required. Thermocyclers with ramp speeds higher than 3°C/sec are considered fast, while slow cyclers

Application Note - mRNA Capture Kit in cDNA-AFLP

suming steps such as washing (Figure 1). In addition, all incubation steps can be performed in a thermocycler. In the work presented here, the effect of gibberellic acid (GA3) treatment or pollination

CpG WIZ® H19-IGF2 Amplification Kit (MilliporeSigma)

or unmodified Normal control DNA) to each control tube. e. Place tubes in the thermocycler block, and perform PCR under the following conditions: Denature: for Taq Polymerase 95°C / 5 minutes

CpG WIZ® MGMT Amplification Kit

(modified U and M, unmodified W) to each control tube. e. Place tubes in the thermocycler block, and perform PCR under the following conditions: 9

TRAPeze® Kit RT Telomerase Detection Kit

the tubes in the thermocycler block. 2. Set-up the real-time experiment to include the following PCR parameters. 30°C 30 min 1 cycle 95°C 2.0 min 1 cycle 45 cycles 94°C

CpG WIZ® hMLH1 Amplification Kit

(modified U and M, unmodified W) to each control tube. e. Place tubes in the thermocycler block, and perform PCR under the following conditions: Denature: for Taq Polymerase 95°C / 5 minutes