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Xuan Jiang et al.
The American journal of pathology, 181(2), 626-641 (2012-06-05)
Mutations in chromosome-helicase-DNA-binding protein 7 (CHD7) are identified as the main cause for CHARGE syndrome (coloboma, heart anomaly, choanal atresia, retardation, genital and ear anomalies). Most patients (55% to 85%) with CHARGE syndrome display developmental defects in the central nervous
Ezgi Hacisuleyman et al.
Nature structural & molecular biology, 21(2), 198-206 (2014-01-28)
RNA, including long noncoding RNA (lncRNA), is known to be an abundant and important structural component of the nuclear matrix. However, the molecular identities, functional roles and localization dynamics of lncRNAs that influence nuclear architecture remain poorly understood. Here, we
Adam D Langenbacher et al.
EvoDevo, 7, 9-9 (2016-04-14)
Germ cells are specified during early development and are responsible for generating gametes in the adult. After germ cells are specified, they typically migrate to a particular niche in the organism where they reside for the remainder of its lifetime.
Adam D Langenbacher et al.
Genesis (New York, N.Y. : 2000), 53(1), 194-201 (2014-09-03)
Botryllus schlosseri is a colonial ascidian with characteristics that make it an attractive model for studying immunology, stem cell biology, evolutionary biology, and regeneration. Transcriptome sequencing and the recent completion of a draft genome sequence for B. schlosseri have revealed
Sonja Oberland et al.
Frontiers in cellular neuroscience, 9, 366-366 (2015-10-07)
Olfactory signals influence food intake in a variety of species. To maximize the chances of finding a source of calories, an animal's preference for fatty foods and triglycerides already becomes apparent during olfactory food search behavior. However, the molecular identity
Camilo Riquelme-Guzmán et al.
eLife, 11 (2022-10-12)
Early events during axolotl limb regeneration include an immune response and the formation of a wound epithelium. These events are linked to a clearance of damaged tissue prior to blastema formation and regeneration of the missing structures. Here, we report
Richard Weiszmann et al.
Nature protocols, 4(5), 605-618 (2009-04-11)
We describe a high-throughput protocol for RNA in situ hybridization (ISH) to Drosophila embryos in a 96-well format. cDNA or genomic DNA templates are amplified by PCR and then digoxigenin-labeled ribonucleotides are incorporated into antisense RNA probes by in vitro
Eunah Chung et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(43), 17732-17737 (2011-10-19)
Estrogen regulates numerous developmental and physiological processes. Most effects are mediated by estrogen receptors (ERs), which function as ligand-regulated transcription factors. Estrogen also regulates the activity of GPR30, a membrane-associated G protein-coupled receptor. Many different types of environmental contaminants can
Yoshihiro Komatsu et al.
Methods in molecular biology (Clifton, N.J.), 1092, 1-15 (2013-12-10)
In situ hybridization is a powerful method for detecting endogenous mRNA sequences in morphologically preserved samples. We provide in situ hybridization methods, which are specifically optimized for mouse embryonic samples as whole mounts and section tissues. Additionally, β-Galactosidase (β-gal) is
Chunyan Li et al.
Nature communications, 12(1), 1094-1094 (2021-02-19)
Seahorses have a circum-global distribution in tropical to temperate coastal waters. Yet, seahorses show many adaptations for a sedentary, cryptic lifestyle: they require specific habitats, such as seagrass, kelp or coral reefs, lack pelvic and caudal fins, and give birth
Susannah H Kassmer et al.
Nature communications, 6, 8565-8565 (2015-10-13)
The colonial ascidian Botryllus schlosseri continuously regenerates entire bodies in an asexual budding process. The germ line of the newly developing bodies is derived from migrating germ cell precursors, but the signals governing this homing process are unknown. Here we
Jie Hong et al.
Experimental eye research, 111, 17-26 (2013-04-02)
A small percentage of retinal ganglion cells (RGCs) express melanopsin and are intrinsically photosensitive (ipRGCs). Whether light can affect the development of ipRGCs is not clear. In the rat retina, we found constant light exposure during the first postnatal week
Yongchao Liu et al.
Scientific reports, 5, 10159-10159 (2015-05-12)
Long non-coding RNAs (lncRNAs), which have evolved as important gene expression modulators, are involved in human malignancies. The down-regulation of lncRNA growth arrest specific transcript 5 (GAS5) has been reported in several cancers, however, the underlying mechanism of lncRNA GAS5
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