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显示 1-30 共 53 条结果 关于 "T3253" 范围 论文
Domenico Azarnia Tehran et al.
Bio-protocol, 8(13), e2918-e2918 (2018-07-05)
Cerebellar Granule Neurons (CGN) from post-natal rodents have been widely used as a model to study neuronal development, physiology and pathology. CGN cultured in vitro maintain the same features displayed in vivo by mature cerebellar granule cells, including the development
Jihwan Lee et al.
Science advances, 6(43) (2020-10-25)
Unraveling the genetic and epigenetic determinants of phenotypes is critical for understanding and re-engineering biology and would benefit from improved methods to separate cells based on phenotypes. Here, we report SPOTlight, a versatile high-throughput technique to isolate individual yeast or
Navaneetha Krishnan Bharathan et al.
Developmental biology, 450(2), 115-131 (2019-04-03)
Desmoplakin (Dsp) is a unique and critical desmosomal protein, that is integral to epidermal development. However, it is unclear whether this protein is required specifically for epidermal morphogenesis. Using morpholinos or Crispr/Cas9 mutagenesis we decreased the function of Dsp in
Alexander Maxan et al.
Neurobiology of disease, 141, 104951-104951 (2020-05-23)
In order to model various aspects of Huntington's disease (HD) pathology, in particular protein spread, we administered adeno-associated virus (AAV) expressing green fluorescent protein (GFP) or GFP coupled to HTT-Exon1 (19Q or 103Q) to the central nervous system of adult
Tibor Maliar et al.
Chemistry & biodiversity, 12(11), 1678-1695 (2015-11-17)
The content of biogenic compounds and the biological activities of barley (Hordeum vulgare L.)-grain extracts was evaluated. The sufficiently large and heterogeneous set of barley genotypes (100 accessions) enabled the selection of special genotypes interesting for potential industrial, pharmaceutical, and
Kyle R Hansen et al.
Journal of neurotrauma, 35(13), 1523-1536 (2018-01-19)
Mild traumatic brain injury (mTBI) represents a serious public health concern. Although much is understood about long-term changes in cell signaling and anatomical pathologies associated with mTBI, little is known about acute changes in neuronal function. Using large scale Ca2+
Vašková Janka et al.
In vitro cellular & developmental biology. Animal, 51(9), 964-974 (2015-05-29)
Although knowledge of cancer management is extensive, mortality is not currently declining in this area. It is therefore important to implement a long-term strategy that would aim to prevent these serious diseases. Ferrocene-related organometallic compounds are promising candidates for design
Cheryl M Armstrong et al.
Toxins, 10(11) (2018-10-27)
Amplified luminescent proximity homogenous assay-linked immunosorbent assay (AlphaLISA) is comprised of a bead-based immunoassay that is used for small molecule detection. In this study, a novel AlphaLISA was developed and optimized for the detection of Shiga-toxin 2 (Stx2). Efficacy and
Carlos Sanchez-Martin et al.
Antioxidants & redox signaling, 34(7), 505-516 (2020-05-23)
Aims: TNF receptor-associated protein 1 (TRAP1), the mitochondrial paralog of the heat shock protein 90 (Hsp90) family of molecular chaperones, is required for neoplastic growth in several tumor cell models, where it inhibits succinate dehydrogenase (SDH) activity, thus favoring bioenergetic
Yuxiang Jiang et al.
Bio-protocol, 7(5), e2146-e2146 (2017-03-05)
Total internal reflection fluorescence (TIRF) microscopy is a powerful tool for visualizing the dynamics of actin filaments at single-filament resolution in vitro. Thanks to the development of various fluorescent probes, we can easily monitor all kinds of events associated with
Arjeta Podrimaj-Bytyqi et al.
Scientific reports, 8(1), 17873-17873 (2018-12-16)
Bladder urothelial cell carcinoma (UCC) is an increasingly prevalent cancer worldwide, and thus, gaining a better understanding of its identifiable risk factors is a global priority. This study addressed this public health need with the understanding that cancer-initiating events, such
Chandrasekhar Natarajan et al.
Current protocols in protein science, 101(1), e112-e112 (2020-07-21)
The development of new technologies for the efficient expression of recombinant hemoglobin (rHb) is of interest for experimental studies of protein biochemistry and the development of cell-free blood substitutes in transfusion medicine. Expression of rHb in Escherichia coli host cells
Bernhard Sissolak et al.
Biotechnology journal, 14(7), e1800714-e1800714 (2019-04-16)
Frequently measured mammalian cell culture process indicators include viability and total cell concentration (TCC). Cell lysis, an additional important process characteristic that substantially contributes to the overall product purity profiles, is often not addressed in detail. In the present study
C Gröer et al.
Journal of pharmaceutical and biomedical analysis, 100, 393-401 (2014-09-15)
Cytochrome P450 (CYP) enzymes and UDP-glucuronosyltransferases (UGT) are major determinants in the pharmacokinetics of most drugs on the market. To investigate their impact on intestinal and hepatic drug metabolism, we developed and validated quantification methods for nine CYP (CYP1A2, CYP2B6
Willy V Bienvenut et al.
Molecular systems biology, 16(7), e9464-e9464 (2020-07-08)
Protein acetylation is a highly frequent protein modification. However, comparatively little is known about its enzymatic machinery. N-α-acetylation (NTA) and ε-lysine acetylation (KA) are known to be catalyzed by distinct families of enzymes (NATs and KATs, respectively), although the possibility
Karol Bomsztyk et al.
Nucleic acids research, 47(12), e69-e69 (2019-03-31)
Chromatin immunoprecipitation (ChIP) is the most widely used approach for identification of genome-associated proteins and their modifications. We have previously introduced a microplate-based ChIP platform, Matrix ChIP, where the entire ChIP procedure is done on the same plate without sample
Marten Szibor et al.
The Journal of biological chemistry, 295(14), 4383-4397 (2020-02-26)
Mitochondrial oxidative phosphorylation (OXPHOS) and cellular workload are tightly balanced by the key cellular regulator, calcium (Ca2+). Current models assume that cytosolic Ca2+ regulates workload and that mitochondrial Ca2+ uptake precedes activation of matrix dehydrogenases, thereby matching OXPHOS substrate supply
Shao-Hua Wu et al.
Biosensors & bioelectronics, 73, 41-46 (2015-06-05)
A new gold particles modified heated copper disk electrode (Au-HCuDE) with direct current was fabricated. The hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) was self-assembled on the heated electrode and resulted in a new biosensor denoted as HRP-DNAzyme/Au-HCuDE. By controlling the temperature
Máté Kiss et al.
Cancer immunology research, 9(3), 309-323 (2020-12-29)
IL1β is a central mediator of inflammation. Secretion of IL1β typically requires proteolytic maturation by the inflammasome and formation of membrane pores by gasdermin D (GSDMD). Emerging evidence suggests an important role for IL1β in promoting cancer progression in patients
Ricky H Bhogal et al.
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, 24(10), 1437-1452 (2018-07-25)
Ischemia/reperfusion injury (IRI) is the main cause of complications following liver transplantation. Reactive oxygen species (ROS) were thought to be the main regulators of IRI. However, recent studies demonstrate that ROS activate the cytoprotective mechanism of autophagy promoting cell survival.
Raquel García-González et al.
Talanta, 142, 11-19 (2015-05-25)
Amplification-free (referring to enzymatic amplification step) detection methodologies are increasing in biosensor development due to the need of faster and simpler protocols. However, for maintaining sensitivity without this step, highly detectable molecules or very sensitive detection techniques are required. The
A M Shibl et al.
Journal of medical microbiology, 13(2), 345-348 (1980-05-01)
The effects of tetracycline, chloramphenicol and polymyxin B on growth and protease production by Pseudomonas aeruginosa were studied. Tetracycline inhibited protease production at concentrations much lower than those required to cause growth inhibition; the effect was not due to inhibition
Tris-tris-HCl: a standard buffer for use in the physiologic pH range.
R A Durst et al.
Clinical chemistry, 18(3), 206-208 (1972-03-01)
Morten Egevang Jørgensen et al.
Bio-protocol, 7(20), e2581-e2581 (2017-10-20)
Xenopus laevis oocytes are a widely used model system for characterization of heterologously expressed secondary active transporters. Historically, researchers have relied on detecting transport activity by measuring accumulation of radiolabeled substrates by scintillation counting or of fluorescently labelled substrates by
Sandra Lawrynowicz Leibel et al.
Scientific reports, 9(1), 13450-13450 (2019-09-19)
Surfactant protein B (SFTPB) deficiency is a fatal disease affecting newborn infants. Surfactant is produced by alveolar type II cells which can be differentiated in vitro from patient specific induced pluripotent stem cell (iPSC)-derived lung organoids. Here we show the
Ying Hou et al.
Biotechnology progress, 31(4), 974-982 (2015-05-29)
Protein A chromatography is widely employed for the capture and purification of monoclonal antibodies (mAbs). Because of the high cost of protein A resins, there is a significant economic driving force to seek new downstream processing strategies. Membrane chromatography has
Cristina Ana Mocanu et al.
Journal of controlled release : official journal of the Controlled Release Society, 338, 754-772 (2021-09-17)
The receptor for advanced glycation end products (RAGE) plays a central role in the chronic inflammatory process associated with atherosclerosis development. We aimed to develop lipoplexes carrying RAGE-short hairpin (sh) RNA, targeted to the adhesion molecule P-selectin, selectively expressed on
Evaluation of the allergenicity and antigenicity of bovine-milk alphas1-casein using extensively purified synthetic peptides.
Elsayed S et al.
Scandinavian Journal of Immunology, 60, 486-486 (2004)
Christian M Schürch et al.
Cell, 182(5), 1341-1359 (2020-08-09)
Antitumoral immunity requires organized, spatially nuanced interactions between components of the immune tumor microenvironment (iTME). Understanding this coordinated behavior in effective versus ineffective tumor control will advance immunotherapies. We re-engineered co-detection by indexing (CODEX) for paraffin-embedded tissue microarrays, enabling simultaneous
Xu Yu et al.
Biosensors & bioelectronics, 66, 520-526 (2014-12-17)
A novel highly sensitive colorimetric assay for DNA detection using cascade amplification strategy based on hybridization chain reaction and enzyme-induced metallization was established. The DNA modified superparamagnetic beads were demonstrated to capture and enrich the target DNA in the hybridization
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