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Jana L Phan et al.
Journal of experimental botany, 67(22), 6481-6495 (2016-11-20)
Xylans are the most abundant non-cellulosic polysaccharide found in plant cell walls. A diverse range of xylan structures influence tissue function during growth and development. Despite the abundance of xylans in nature, details of the genes and biochemical pathways controlling
Henning Willers et al.
Journal of biomedicine & biotechnology, 2008, 821529-821529 (2008-05-17)
Genetic or epigenetic inactivation of the pathway formed by the Fanconi anemia (FA) and BRCA1 proteins occurs in several cancer types, making the affected tumors potentially hypersensitive to DNA cross-linkers and other chemotherapeutic agents. It has been proposed that the
Yung-Hsin Cheng et al.
Experimental eye research, 179, 179-187 (2018-11-25)
Elevated intraocular pressure (IOP) in glaucoma is due to impairment of aqueous humor drainage via the uveoscleral or trabecular outflow pathway. Latanoprost reduces IOP by increasing the uveoscleral outflow. Despite its potency, long-term daily application of it may cause undesirable
Alireza Pouya et al.
PloS one, 6(11), e27925-e27925 (2011-11-30)
This study aims to differentiate human induced pluripotent stem cells (hiPSCs) into oligodendrocyte precursors and assess their recovery potential in a demyelinated optic chiasm model in rats. We generated a cell population of oligodendrocyte progenitors from hiPSCs by using embryoid
Arkadiusz Urbański et al.
Micron (Oxford, England : 1993), 104, 8-20 (2017-10-20)
The evolutionary success of insects is undoubtedly related to a well-functioning immune system. This is especially apparent during insect development by the adaptation of individuals to the changing risk of infection. In addition, current studies show that the insect immune
Marita Pall et al.
Fertility and sterility, 91(6), 2551-2556 (2008-06-17)
To test the hypothesis that DHEAS production from DHEA occurs in hepatic cells and that this production is augmented by the presence of sex steroids or insulin. In vitro prospective experiment. Academic medical center. Hepatoma G2 cells cultured in media
Remedios Castelló-Cros et al.
Methods in molecular biology (Clifton, N.J.), 522, 275-305 (2009-02-28)
It is increasingly recognized that interactions between cancer cells and their surrounding stroma are critical for promoting the growth and invasiveness of tumors. For example, cancer cells alter the topography and molecular composition of stromal extracellular matrix by increasing paracrine
A Urbański et al.
Developmental and comparative immunology, 120, 104065-104065 (2021-03-12)
Tachykinin-related peptides (TRPs) are important neuropeptides. Here we show that they affect the insect immune system, especially the cellular response. We also identify and predict the sequence and structure of the tachykinin-related peptide receptor (TRPR) and confirm the presence of
Somayeh Karimzadeh et al.
European journal of cell biology, 97(2), 126-135 (2018-02-06)
Recent evidence suggests that mitochondrial apoptosis regulators and executioners may regulate differentiation, without being involved in cell death. However, the involved factors and their roles in differentiation and apoptosis are still not fully determined. In the present study, we compared
Juan C Calderón et al.
The Journal of physiology, 588(Pt 1), 267-279 (2009-11-04)
Electrically elicited Ca(2+) transients reported with the fast Ca(2+) dye MagFluo-4 AM and myosin heavy chain (MHC) electrophoretic patterns were obtained in intact, enzymatically dissociated fibres from adult mice extensor digitorum longus (EDL) and soleus muscles. Thirty nine fibres (23
Somin Eunice Lee et al.
Nano letters, 15(7), 4564-4570 (2015-06-04)
Plasmon rulers, consisting of pairs of gold nanoparticles, allow single-molecule analysis without photobleaching or blinking; however, current plasmon rulers are irreversible, restricting detection to only single events. Here, we present a reversible plasmon ruler, comprised of coupled gold nanoparticles linked
H Choudhry et al.
Oncogene, 34(34), 4482-4490 (2014-11-25)
Activation of cellular transcriptional responses, mediated by hypoxia-inducible factor (HIF), is common in many types of cancer, and generally confers a poor prognosis. Known to induce many hundreds of protein-coding genes, HIF has also recently been shown to be a
Eung-Soo Hwang et al.
Journal of virology, 83(23), 12388-12398 (2009-09-25)
Infection of host cells with human cytomegalovirus (HCMV) induces cell cycle dysregulation. Two HCMV immediate-early (IE) proteins, IE1-72 and IE2-86, are promiscuous transactivators that have been implicated in the dysregulatory events. Cellular p53 protein is accumulated to high levels in
A simple, rapid and non-destructive procedure to extract cell wall-associated proteins from Frankia.
F Tavares et al.
Journal of microbiological methods, 39(2), 171-178 (1999-11-27)
A simple cell fractionation procedure was developed to extract cell wall-associated proteins from the nitrogen-fixing actinomycete Frankia. The method was based on washing Frankia mycelia in 62.5 mM Tris-HCl (pH 6.8) buffer supplemented with 0.1% Triton X-100 as solubilizing agent.
Samina Alam et al.
Molecular cancer, 10, 97-97 (2011-08-11)
In normal cells proliferation and apoptosis are tightly regulated, whereas in tumor cells the balance is shifted in favor of increased proliferation and reduced apoptosis. Anticancer agents mediate tumor cell death via targeting multiple pathways of programmed cell death. We
Wei Xiong et al.
Nature protocols, 9(10), 2438-2449 (2014-09-19)
Mechanosensation, the transduction of mechanical force into electrochemical signals, allows organisms to detect touch and sound, to register movement and gravity, and to sense changes in cell volume and shape. The hair cells of the mammalian inner ear are the
Matthew J Stroud et al.
The Journal of biological chemistry, 286(28), 24987-24995 (2011-05-13)
The microtubule (MT) and actin cytoskeletons are fundamental to cell integrity, because they control a host of cellular activities, including cell division, growth, polarization, and migration. Proteins involved in mediating the cross-talk between MT and actin cytoskeletons are key to
Nidal Ghosheh et al.
Stem cells international, 2016, 8648356-8648356 (2016-03-08)
Human pluripotent stem cells- (hPSCs-) derived hepatocytes have the potential to replace many hepatic models in drug discovery and provide a cell source for regenerative medicine applications. However, the generation of fully functional hPSC-derived hepatocytes is still a challenge. Towards
Ewa K Zuba-Surma et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 73A(12), 1116-1127 (2008-10-28)
Recently, we purified a population of CXCR4+/Oct-4+/SSEA-1+/Sca-1+/Lin(-)/CD45(-) very small embryonic-like stem cells (VSELs) from adult murine bone marrow (BM). After using flow cytometry, ImageStream analysis, confocal microscopy, and real time RT-PCR, we report that similar cells could be also identified
Cedric Auffray et al.
The Journal of experimental medicine, 206(3), 595-606 (2009-03-11)
CX(3)CR1 expression is associated with the commitment of CSF-1R(+) myeloid precursors to the macrophage/dendritic cell (DC) lineage. However, the relationship of the CSF-1R(+) CX(3)CR1(+) macrophage/DC precursor (MDP) with other DC precursors and the role of CX(3)CR1 in macrophage and DC
Zbigniew Darzynkiewicz et al.
Methods (San Diego, Calif.), 44(3), 250-254 (2008-03-04)
Activation of endonucleases that cleave chromosomal DNA preferentially at internucleosomal sections is a hallmark of apoptosis. DNA fragmentation revealed by the presence of a multitude of DNA strand breaks, therefore, is considered to be the gold standard for identification apoptotic
Baydaa El Shazely et al.
Insect biochemistry and molecular biology, 110, 60-68 (2019-05-06)
Antimicrobial peptides (AMPs) are important immune effectors in insects. Bacteria have a limited number of ways to resist AMPs, and AMP-resistance is often costly. Recently, it has become clear that AMP activities in vitro and in vivo differ. Although some
Sivaram Harendra et al.
Journal of environmental sciences (China), 25(3), 576-584 (2013-08-08)
Surfactants are soil washing agents and facilitators for subsurface remediation of hydrocarbon spills. It is important to understand the sorption and transport behavior of surfactants for enhanced soil remediation. The adsorption and desorption isotherms of cetyl trimethylammonium bromide (CTAB) and
Wan-Heng Wang et al.
The Journal of clinical investigation, 118(3), 1056-1064 (2008-02-16)
Elevated intraocular pressure (IOP) is the principal risk factor for glaucoma and results from excessive impedance of the fluid outflow from the eye. This abnormality likely originates from outflow pathway tissues such as the trabecular meshwork (TM), but the associated
Hadi Shafiee et al.
Scientific reports, 5, 9919-9919 (2015-06-06)
We report a biosensing platform for viral load measurement through electrical sensing of viruses on a flexible plastic microchip with printed electrodes. Point-of-care (POC) viral load measurement is of paramount importance with significant impact on a broad range of applications
C Chan et al.
Electrophoresis, 20(17), 3467-3471 (1999-12-23)
An optimised protocol for the production of two-dimensional protein patterns of human colonic mucosal cells using immobilised pH gradients in the first dimension is presented. We tested a wide variety of solubilising agents and electrophoretic parameters, separately and in combination.
Lalji K Gediya et al.
Bioorganic & medicinal chemistry, 16(6), 3352-3360 (2008-01-02)
We have developed new, simple, and efficient procedures for the synthesis of two promising histone deacetylase inhibitors (HDIs), CI-994, (N-(2-aminophenyl)-4-acetylaminobenzamide), and MS-275 (N-(2-aminophenyl)4-[N-(pyridine-3-yl-methoxycarbonyl)aminomethyl]benzamide) from commercially available acetamidobenzoic acid and 3-(hydroxymethyl)pyridine, respectively. The procedures provide CI-994 and MS-275 in 80% and
Yuan Lyu et al.
Cells, 9(10) (2020-10-11)
Blood extracellular vesicles (BEVs) carry bioactive cargo (proteins, genetic materials, lipids, licit, and illicit drugs) that regulate diverse functions in target cells. The cannabinoid drug delta-9-tetrahydrocannabinol (THC) is FDA approved for the treatment of anorexia and weight loss in people
Lígia Sousa-Ferreira et al.
PloS one, 6(5), e19745-e19745 (2011-05-19)
Some pathological conditions with feeding pattern alterations, including obesity and Huntington disease (HD) are associated with hypothalamic dysfunction and neuronal cell death. Additionally, the hypothalamus is a neurogenic region with the constitutive capacity to generate new cells of neuronal lineage
Chia-Yuan Liu et al.
Radiation oncology (London, England), 6, 56-56 (2011-06-01)
This work aimed to investigate the effect of costunolide, a sesquiterpene lactone isolated from Michelia compressa, on cell cycle distribution and radiosensitivity of human hepatocellular carcinoma (HCC) cells. The assessment used in this study included: cell viability assay, cell cycle
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