T9410

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Absolute Quantification of the Central Carbon Metabolome in Eight Commonly Applied Prokaryotic and Eukaryotic Model Systems.

Lisa M Røst et al.

Metabolites, 10(2) (2020-02-26)

Absolute quantification of intracellular metabolite pools is a prerequisite for modeling and in-depth biological interpretation of metabolomics data. It is the final step of an elaborate metabolomics workflow, with challenges associated with all steps-from sampling to quantifying the physicochemically diverse

Survival of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis frozen in skim milk- tryptone-glucose-glycerol medium.

Tarja Kaijalainen et al.

Journal of clinical microbiology, 42(1), 412-414 (2004-01-13)

In STGG (skim milk, tryptone, glucose, glycerol) medium at -80 degrees C, Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis isolates survived for at least 3 years, and the same species have survived in nasopharyngeal swabs for at least 1.5 years.

Nitrogen source and concentration affect utilization of glucose by mixed ruminal microbes in vitro.

M B Hall

Journal of dairy science, 100(4), 2739-2750 (2017-02-22)

The availability of rumen-degradable protein (RDP) changes the use of carbohydrates by ruminal microbes. However, the effects of RDP on the simultaneous use of carbohydrate and formation of microbial products are not well described, although such information is needed to

Estimating degradation of individual essential amino acids in fish meal and blood meal by rumen microbes in a dual-flow continuous-culture system.

S Gargallo et al.

Journal of dairy science, 103(7), 6209-6217 (2020-04-26)

Current feeding systems are based on the assumption that the AA profile of rumen undegraded protein is similar to that of the original feed. The objective of this experiment was to determine rumen bacterial degradation of individual essential AA in

Long-term monitoring of bacteria undergoing programmed population control in a microchemostat.

Frederick K Balagaddé et al.

Science (New York, N.Y.), 309(5731), 137-140 (2005-07-05)

Using an active approach to preventing biofilm formation, we implemented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small populations of bacteria with single-cell resolution. We used this device to observe the dynamics of Escherichia coli carrying

Optimizing conditions for the growth of Lactobacillus casei YIT 9018 in tryptone-yeast extract-glucose medium by using response surface methodology.

S Oh et al.

Applied and environmental microbiology, 61(11), 3809-3814 (1995-11-01)

This study was undertaken to find optimum conditions of tryptone, yeast extract, glucose, Tween 80, and incubation temperature for the growth of Lactobacillus casei YIT 9018 and to assess the effects of these factors by use of response surface methodology.

Divergent utilization patterns of grass fructan, inulin, and other nonfiber carbohydrates by ruminal microbes.

M B Hall et al.

Journal of dairy science, 99(1), 245-257 (2015-11-26)

Fructans are an important nonfiber carbohydrate in cool season grasses. Their fermentation by ruminal microbes is not well described, though such information is needed to understand their nutritional value to ruminants. Our objective was to compare kinetics and product formation

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