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Hamza Ali et al.
Journal of medical microbiology, 63(Pt 6), 884-891 (2014-03-29)
Bacterial contamination of platelet concentrates (PCs) poses the highest transfusion-associated infectious risk, with Staphylococcus epidermidis being a predominant contaminant. Herein, the growth dynamics of 20 S. epidermidis strains in PCs and regular media were characterized. Strains were categorized as fast
Mao-Tian Zhou et al.
Molecular & cellular proteomics : MCP, 14(9), 2535-2549 (2015-07-01)
Compared with whole-cell proteomic analysis, subcellular proteomic analysis is advantageous not only for the increased coverage of low abundance proteins but also for generating organelle-specific data containing information regarding dynamic protein movement. In the present study, peroxisome-enriched fractions from Sendai
Daniel Anthony Dias et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1000, 1-13 (2015-07-24)
This study reports a GC-QqQ-MS method for the quantification of forty-eight primary metabolites from four major classes (sugars, sugar acids, sugar phosphates, and organic acids) which can be applied to a number of biological systems. The method was validated in
Nan Li et al.
Immunology and cell biology, 93(3), 311-320 (2014-11-19)
The mammalian testis is an immunoprivileged organ where male germ cell autoantigens are immunologically ignored. Both systemic immune tolerance to autoantigens and local immunosuppressive milieu contribute to the testicular immune privilege. Testicular immunosuppression has been intensively studied, but information on
John R P Knight et al.
The Biochemical journal, 465(2), 227-238 (2014-10-30)
Cells respond to external stress conditions by controlling gene expression, a process which occurs rapidly via post-transcriptional regulation at the level of protein synthesis. Global control of translation is mediated by modification of translation factors to allow reprogramming of the
Anja K K Rahn et al.
Food chemistry, 166, 301-308 (2014-07-24)
Sodium chloride has been shown to promote chlorination of glycerol during thermal processing. However, the detailed mechanism of this reaction is not well understood. Preliminary experiments have indicated that the reaction mixture should contain an amino acid and it should
Zohreh Hosseinzadeh et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 36(2), 727-740 (2015-05-30)
Janus kinase-3 (JAK3) is activated during energy depletion. Energy-consuming pumps include the Na(+)/K(+)-ATPase. The present study explored whether JAK3 regulates Na(+)/K(+)-ATPase in dendritic cells (DCs). Ouabain (100 µM)-sensitive (Iouabain) and K(+)-induced (Ipump) outward currents were determined by utilizing whole cell
Hongying Zhang et al.
Plant signaling & behavior, 6(5), 652-654 (2011-03-31)
Drought, salinity and low temperature are major environmental factors that influence plant growth and development, and eventually limit crop yield and quality. To survive adverse stresses, plants have developed complex signaling networks to perceive external stimuli, and then manifest adaptive
Xu Chen et al.
Journal of biomedical materials research. Part A, 103(12), 3886-3895 (2015-07-01)
There is considerable interest in how the fate of adipose-derived stem cells is determined. Physical stimuli play a crucial role in skeletogenesis and in cartilage repair and regeneration. In the present study, we investigated the comparative and interactive effects of
Shang Song et al.
Acta biomaterialia, 18, 100-111 (2015-03-05)
Critical failures associated with current engineered bone grafts involve insufficient induction of osteogenesis of the implanted cells and lack of vascular integration between graft scaffold and host tissue. This study investigated the combined effects of surface microtextures and biochemical supplements
Brahim El Mathari et al.
Human molecular genetics, 24(14), 3939-3947 (2015-04-23)
We have previously shown that the deletion of the dystrophin Dp71 gene induces a highly permeable blood-retinal barrier (BRB). Given that BRB breakdown is involved in retinal inflammation and the pathophysiology of many blinding eye diseases, here we investigated whether
Yu-Min He et al.
Journal of natural medicines, 69(3), 303-312 (2015-03-10)
Gentianae Scabrae Radix is a well-known traditional medicine that is used for the treatment of hepatitis, cholecystitis and inflammatory diseases. It consists mainly of secoiridoid glycosides, with representatives of gentiopicroside, sweroside and swertiamarin. In the present study, a chemical investigation
S Watanabe et al.
Cell death & disease, 5, e1497-e1497 (2014-10-31)
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the selective and progressive loss of motor neurons. Cystatin C (CysC), an endogenous cysteine protease inhibitor, is a major protein component of Bunina bodies observed in the spinal motor
Simon Henriet et al.
Nucleic acids research, 43(7), 3701-3711 (2015-03-18)
Selective pressure to maintain small genome size implies control of transposable elements, and most old classes of retrotransposons are indeed absent from the very compact genome of the tunicate Oikopleura dioica. Nonetheless, two families of retrotransposons are present, including the
Madoka Nakai et al.
The Journal of general virology, 96(Pt 4), 904-914 (2014-12-20)
A granulovirus (GV) producing occlusion bodies (OBs) with an unusual appearance was isolated from Adoxophyes spp. larvae in the field. Ultrastructural observations revealed that its OBs were significantly larger and cuboidal in shape, rather than the standard ovo-cylindrical shape typical
Ivana D'Andrea et al.
EMBO molecular medicine, 7(7), 904-917 (2015-04-18)
Although PI3Kγ has been extensively investigated in inflammatory and cardiovascular diseases, the exploration of its functions in the brain is just at dawning. It is known that PI3Kγ is present in neurons and that the lack of PI3Kγ in mice
Muhammad Shahid et al.
The journal of physical chemistry. B, 119(25), 8072-8079 (2015-06-13)
An enhanced thermal decomposition of chemical compounds in aqueous solution has been achieved at reduced solution temperatures. The technique exploits hitherto unrecognized properties of a bubble column evaporator (BCE). It offers better heat transfer efficiency than conventional heat transfer equipment.
Xiaoyan Chu et al.
Drug metabolism and disposition: the biological fate of chemicals, 43(6), 851-863 (2015-03-31)
Inhibition of hepatic transporters such as organic anion transporting polypeptides (OATPs) 1B can cause drug-drug interactions (DDIs). Determining the impact of perpetrator drugs on the plasma exposure of endogenous substrates for OATP1B could be valuable to assess the risk for
Ying Huo et al.
Cryobiology, 95, 20-28 (2020-07-01)
To evaluate the long-term effects of oocyte cryopreservation on the health of the first filial generation (F1), we used B6D2F1 mice for oocyte collection, in vitro fertilization, and breeding. The female F1 mice born from the offspring of fresh mature
Cesare Castellini et al.
Systems biology in reproductive medicine, 60(3), 143-150 (2014-02-20)
Using the rabbit as an animal model, this study evaluated the long-term effect of silver nanoparticles (NPs) administered intravenously (0.6 mg/kg bw) on reproductive activity and sperm quality. Semen analysis was performed by optical microscopy and sperm motility evaluation by
María Sol Balbuena et al.
The Journal of experimental biology, 218(Pt 17), 2799-2805 (2015-09-04)
Glyphosate (GLY) is a herbicide that is widely used in agriculture for weed control. Although reports about the impact of GLY in snails, crustaceans and amphibians exist, few studies have investigated its sublethal effects in non-target organisms such as the
Hiromi Murase et al.
Investigative ophthalmology & visual science, 56(4), 2511-2518 (2015-03-26)
Renewal and elimination of the aged photoreceptor outer segment (POS) by RPE cells is a daily rhythmic process that is important for long-term vision. Phagocytic dysfunction results in photoreceptor cell death. Tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, is known
Jan P Böttcher et al.
Journal of immunology (Baltimore, Md. : 1950), 195(10), 5066-5076 (2015-10-16)
Dendritic cells (DCs) are powerful APCs that can induce Ag-specific adaptive immune responses and are increasingly recognized as important players in innate immunity to both infection and malignancy. Interestingly, although there are multiple described hematological malignancies, DC cancers are rarely
Jie Wen et al.
Neuropharmacology, 99, 196-209 (2015-07-21)
Alpha/beta-hydrolase domain 6 (ABHD6) is a novel 2-arachidonoylglycerol (2-AG) hydrolytic enzyme, that can fine-tune the endocannabinoid signaling in the central nervous system. Recently we and others have demonstrated the protective effect of ABHD6 inhibition in the animal models of traumatic
Alexander J Lausch et al.
Biomacromolecules, 16(7), 1938-1947 (2015-05-27)
The periodontium is the set of tissues responsible for tooth anchorage, and consists of interconnected layers of mineralized and unmineralized tissues (bone, ligament and cementum). The ligament-cementum interface is a particularly elegant example of biological control of mineralization and the
Kimberly J Hassett et al.
Journal of pharmaceutical sciences, 104(2), 627-639 (2015-01-13)
During transport and storage, vaccines may be exposed to temperatures outside of the range recommended for storage, potentially causing efficacy losses. To better understand and prevent such losses, dominant negative inhibitor (DNI), a recombinant protein antigen for a candidate vaccine
Martín García-Flores et al.
Journal of agricultural and food chemistry, 63(3), 1042-1052 (2015-01-15)
In comparison to the exponential increase of genotyping methods, phenotyping strategies are lagging behind in agricultural sciences. Genetic improvement depends upon the abundance of quantitative phenotypic data and the statistical partitioning of variance into environmental, genetic, and random effects. A
Shaowen Wang et al.
Journal of virology, 88(22), 13047-13063 (2014-08-29)
Iridoviruses are nucleocytoplasmic DNA viruses which cause great economic losses in the aquaculture industry but also show significant threat to global biodiversity. However, a lack of host cells has resulted in poor progress in clarifying iridovirus behavior. We investigated the
Steven D Zink et al.
Viruses, 7(10), 5619-5631 (2015-10-31)
Complex interactions between microbial residents of mosquitoes and arboviruses are likely to influence many aspects of vectorial capacity and could potentially have profound effects on patterns of arbovirus transmission. Such interactions have not been well studied for West Nile virus
Adam P Bateman et al.
The journal of physical chemistry. A, 119(19), 4386-4395 (2014-11-15)
The effect of relative humidity (RH) on the rebound of particles composed of isoprene, α-pinene, and toluene secondary organic materials (SOMs) was studied. A three-arm impaction apparatus was used to study rebound from 5 to 95% RH at 298 K.
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