- A generalized strategy for immobilizing uniformly oriented membrane proteins at solid interfaces.
A generalized strategy for immobilizing uniformly oriented membrane proteins at solid interfaces.
Chemical communications (Cambridge, England) (2013-02-26)
Amit Vaish, Vitalii Silin, Marlon L Walker, Kristen L Steffens, Susan Krueger, Alexei A Yeliseev, Klaus Gawrisch, David J Vanderah
PMID23435270
摘要
We have developed a method based on self-assembly of thiols on Au substrates to immobilize membrane proteins at interfaces. Using water soluble nitrilotriacetic acid (NTA)-terminated oligo(ethylene glycol) thiols, a histidine-tagged G protein-coupled membrane receptor (GPCR) was captured in a defined orientation with little nonspecific binding.
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Supelco
乙二醇 溶液, suitable for NMR (reference standard), 80% in DMSO-d6 (99.9 atom % D), NMR tube size 5 mm × 8 in.