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Immobilon® Tools

for Western blotting

Tailored for Your Western Blot

Western blotting is an important technique when you have a complex sample and want to identify a specific protein.

Successful Western blot experiments rely on having the right tools for the job.

Our comprehensive Immobilon® portfolio of products is designed for a dependable, reproducible, and high-sensitivity Western blotting experiment.

The product range offers the following:

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Move your research forward by evaluating novel, updated electrophoresis and Western blotting workflow products that deliver improved results in less time.

  • TurboMix™ Bis-Tris Polyacrylamide Gel Casting Kits deliver a rapid and reproducible workflow for hand-cast protein gels. Bis-tris gel chemistry has a similar molecular weight range to standard tris-glycine, but provides multiple benefits: faster run time, sharper protein bands, and longer shelf life. Learn More
  • mPAGE® Bis-Tris Polyacrylamide Gels provide outstanding resolution, short run times, and are compatible with popular gel tanks. Trial kits include 2 gels and running buffer powder to make 1L tank adapters. Learn More
  • ReadyBlue™ Protein Gel Stain is a rapid and sensitive colloidal Coomassie stain for polyacrylamide gels. The stain is room-temperature stable and ready to use, allowing for a fast and simplified protocol with results in 10 minutes or less. No prior fixing step is necessary.
  • Immobilon® PVDF Transfer Membranes offer high protein binding capacity for improved signal. The durability and high protein retention of Immobilon® PVDF membranes make them the preferred option for the sequential detection of multiple targets via antibody stripping and reprobing. Learn More
  • Immobilon® Chemiluminescent Substrates: we offer a broad selection of ECL (enhanced chemiluminescence) substrates for every application. Immobilon® ECL Ultra delivers the highest sensitivity and enables reduced antibody consumption. Immobilon® Forte, Crescendo, and Classico provide the convenience and reliability of premixed, one-component solutions with good performance. Learn More
  • Immobilon® Signal Enhancer combines signal amplification and blocking in one ready-to-use reagent. It boosts chemiluminescent and fluorescent signals and enables reduced antibody consumption. Block Noise Cancelling reagents provide protein-free blocking for Western blot experiments in a convenient, ready-to-use room temperature stable formulations.

Explore Our PVDF and Nitrocellulose Membranes

  • Immobilon®-P membranes: Our classic PVDF membrane offers high protein binding for greater sensitivity as well as durability for stripping and re-probing. Compatible with chromogenic and chemiluminescent detection.
  • Immobilon®-E membranes : The only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step. This membrane is well-suited for most Western blotting applications and is compatible with chromogenic and chemiluminescent detection.
  • Immobilon®-PSQ membranes: Higher binding capacity and retention for stronger signal, making these membranes ideal for protein sequencing and immunoblotting of low molecular weight proteins (<20 kDa).
  • Immobilon®-FL membranes: Developed for low background across a wide range of excitation and emission wavelengths for fluorescence-based detection protocols, these membranes are ideal for multiplexing, fluorescence, and chemifluorescence detection methods as well as non-fluorescent detection chemistries.
  • Immobilon® NC membranes: Our nitrocellulose membranes offer lowest background with decreased non-specific antibody binding and can bind both proteins and nucleic acids for a variety of blotting applications.
Immobilon NOW

Sliced to Your Need: Immobilon® NOW Transfer Membrane Rolls and Dispenser

Immobilon® NOW transfer membranes offer the convenience of pre-cut sheets with the flexibility of rolls in the formats you depend on: Immobilon®-E, Immobilon®-P, Immobilon®-FL Immobilon®-PSQ, and Immobilon®-NC membranes and provide

  • Single-cut convenience: measure, cut, and store the transfer membrane in the Immobilon® NOW Dispenser
  • Compatible with mini and midi gels
  • No waste! Use just what you need.
  • Compact design saves laboratory space.
Immobilon® ECL Substrates for Superior Performance

Superior Performance with Immobilon® ECL Substrates

Immobilon® ECL (enhanced chemiluminescence) Ultra and Western HRP substrates deliver exceptional sensitivity and long signal life. Our range of substrates offers the following:

  • Single-component, ready-to-use formulations to simplify detection and provide enhanced reproducibility.
  • Two-component formulations render the highest sensitivity and long signal duration, thereby saving antibodies during detection.
  • A broad range of choices provides an optimal signal-to-background ratio across a spectrum of target protein concentrations.

30 Minutes to Detect Your Choice of Protein

Are you tired of immunodetection protocols that require lots of hands-on time? 

Not to worry!

Explore our  SNAP i.d.® 2.0 system for an accelerated immunodetection step in your Western blotting experiment. The SNAP i.d.® 2.0 system uses a vacuum manifold to drive blocking reagents and antibodies and wash buffers directly through the PVDF or nitrocellulose membrane to speed up the workflow.

The rapid immunodetection system:

  • Shortens cycle time from 4-24 hours to just 30 minutes.
  • Enables parallel processing of blots, making it easy to apply consistent conditions across experiments.
Related Applications
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
Lysis & Protein Extraction
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Related Product Categories
Transfer Membranes & Kits
Choose from a variety of Immobilon® PVDF membranes for optimal Western blotting and protein immunoblotting performance based on target protein size, application, detection method, and convenience.
Immunodetection Reagents & Substrates
High quality blocking reagents, secondary antibodies, and HRP and AP substrates for Western blotting and immunoblotting detection.
Membrane Immunodetection Devices
Simplify your Western blotting workflow and save time using immunodetection devices for Western blot blocking, antibody binding, and washing.
Transfer Buffers & Reagents
Premixed transfer buffers for consistent, reliable protein transfer and high purity, electrophoresis-grade chemicals for making transfer buffers for Western blotting applications.
Protein Gel Electrophoresis and Transfer Equipment
Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.
Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Protein Electrophoresis Gel Stains
High sensitivity colorimetric and fluorescent protein gel stains, fixing solutions, and de-staining reagents for polyacrylamide gels, agarose gels, and PVDF and nitrocellulose membranes.
Protein Markers
Pre-stained and unstained molecular weight markers for protein gel electrophoresis, SDS-PAGE, and Western blotting applications.
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