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Separation Options with Sephacryl®
Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.
Performing a Purification and On-column Refolding of an Insoluble Histidine-tagged Protein
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.
Removal of GST Tag by Enzymatic Cleavage
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Applying Equilibrated Immobiline® DryStrip Gels to SDS Gels
This page shows how to apply equilibrated Immobiline DryStrip gels
High-Performance Thin-Layer Chromatography: A Fast and Efficient Fingerprint Analysis Method for Medicinal Plants
In this article we present several HPTLC applications and analytical standards for ginsenosides.
Minipreps using His SpinTrap™ and His SpinTrap™ Kit
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.
Fast LC-MS-MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3
We presents a robust method for analyzing 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in serum using a multiplexed LC-MS-MS system.
Multiplexing Metabolic Syndrome Biomarkers | MILLIPLEX® Assays
Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
Gravity-flow Purification using His GraviTrap™ and His GraviTrap™ Kit
This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from Cytiva.
Immunohistochemistry Applications in Gastrointestinal Cancer
Colorectal cancer is the third most common cancer in both men and women. An estimated 136,000 cases of colorectal cancer are expected to occur in 2016.
Dephosphorylation Procedures for DNA and Proteins
Dephosphorylation of DNA using Calf Intestinal Alkaline Phosphatase, Shrimp Alkaline Phospha, Bovine Intestinal Alkaline Phosphatase
A Simple Strategy for Protein Enrichment Using Ultrafiltration
The human plasma proteome is a vast resource for biomarker discovery and investigation.
Cultrex® 3-D Culture Matrix™ Laminin I Protocol
3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.
Immunohistochemistry: Current Applications in Skin Cancer
Immunohistochemistry (IHC) techniques and applications have greatly improved, dermatopathology is still largely based on H&E stained slides.This paper outlines ways in which IHC antibodies can be utilized for dermatopathology.
Determination of 2-Chloroethanol as Marker for Fumigant Ethylene Oxide in Sesame Seeds by HS-SPME-GC-MS
The work describes the development of an HS-SPME-GC-MS method to determine 2-chloroethanol as a marker of fumigant ethylene oxide in different kinds of sesame seed samples.
BINOL and Derivatives
We present an article concerning BINOL and Derivatives.
Advancements in the Fabrication of Integrated Organic Electronics
Professor Tokito and Professor Takeda share their new materials, device architecture design principles, and performance optimization protocols for printed and solution-processed, low-cost, highly flexible, organic electronic devices.
How to Make and Use Percoll Gradients
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
GC Analysis of Omega 3 Fatty Acids in Fish Oil Capsules and Farm Raised Salmon
nalysis of the omega 3 fatty acid profiles from fish oil capsules and salmon, highlighted through the use of 2 GC columns of different selectivity for analysts who wish to perform confirmatory analysis.
RPC Purification Options and Scale up with SOURCE™ Media
The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up
LC/MS/MS Method for Determination of Glyphosate, AMPA, and Glufosinate in Cereals
Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.
Lipoprotein Function
Learn about the five main classes of lipoproteins: chylomicrons, very low density lipoproteins (VLDL), intermediate density lipoproteins (IDL), low density lipoproteins (LDL) and lipoprotein(a), and high density lipoproteins.
QuickLink™ DNA Ligation Kit
T4 DNA ligase is used for the joining of DNA molecules with compatible cohesive (sticky) termini, joining of blunt ended double stranded DNA molecules
Auto2D® 2-D Gel Electrophoresis Device Frequently Asked Questions
Auto2D® 2-D Gel Electrophoresis Device frequently asked questions for 2-D PAGE and additional 2DE protein separation workflow needs.
Tips & Tricks | Sample Handling and Preparation | MILLIPLEX® Assays
Have questions about sample handling and preparation for MILLIPLEX® multiplex panels? Explore information on sample collection, sample handling, and sample preparation for multiplex assays with tips and tricks to get the most out of your assays.
ATRP Ligands and Initiators for the Clean Synthesis of Functional Polymers
Atom transfer radical polymerization (ATRP) has emerged as one of the most successful synthetic techniques for the preparation of polymers with predetermined molecular weights, narrow molecular weight distributions, and high degrees of chain end functionalities.
Biomedical Implant Devices Fabricated from Low Young’s Modulus Titanium Alloys Demonstrating High Mechanical Biocompatibility
Biomedical implants are essentially foreign substances within the human body that must survive many years’ exposure to demanding mechanical and physiological conditions. Despite these challenges, metal implants have been widely used to substitute for or rebuild hard tissues such as
Self-Assembled Monolayers: Advantages of Pure Alkanethiols
We presents an article regarding Self-Assembled Monolayers: Advantages of Pure Alkanethiols.
Smart Oligonucleotide Delivery by Multifunctional Block Copolymers
Kanjiro Miyata (The University of Tokyo, Japan) provides insights on the rational design of polymeric materials for “smart” oligonucleotide delivery.
Understanding Sweet Taste Perception
The powerful effect of the pleasurable sensation arising from sweet tasting foods has led to consumer demand and large-scale production of the caloric sweetening agents sucrose and fructose.
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