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3D Mammosphere Culture Media

3D mammosphere culture protocol used to expand human MCF-7 and MDA-MB-231 breast cancer cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

Determination of Caffeine in Energy Drinks with HPTLC-MS

Thin layer chromatography coupled with mass spectrometry does not require substantial sample preparation. Energy drinks can be applied without dilution directly onto the TLC plate.

Enzymatic Assay of Invertase

Optimizing Crude Sample Plant PCR

Overcoming potent inhibitors and sample diversity in direct plant PCR.

Applying Equilibrated Immobiline® DryStrip Gels

This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.

Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

HPLC Method for the Separation of Nine Key Silymarin Components of Milk Thistle

Experimental steps, coupled with prediction software, provided conditions suitable for the analysis biologically active milk thistle components.

Blood Brain Barrier Antibody Penetration Assay using Millicell® Microwell Plates

Discover a high throughput assay using organoids and Millicell® Microwell plates. Our novel blood brain barrier model allows direct cell-to-cell interactions, closely modeling the in vivo properties.

Determination of Omeprazole and Related Compounds

The Ascentis® Express 120 Å C18 pH+ column delivers high efficiency, stable retention, and reliable peak performance under alkaline mobile phase conditions.

Improving API Solubility Using Spray Drying with Polyvinyl Alcohol

Discover how spray drying with polyvinyl alcohol enhances solubility for small and large molecule drugs and unlocks the potential of poorly soluble APIs.

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