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Large molecule HPLC
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facet applications:Low pressure liquid chromatography
facet applications:Small molecule HPLC
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Showing 31-60 of 198 results
Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
SupraSolv® GC Solvent Specifications
Our GC solvents have been specially developed and tested for ECD, FID and MS detection or for headspace gas chromatography to ensure accurate, reliable and reproducible analytical results.
Synthetic Cannabinoid Analysis
A large variety of cannabinoid drug standards, along with an HPLC method with the Supelco Ascentis® Express RP-Amide column, are presented in this article.
Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
Optimising the LC-MS Analysis of Biomolecules
We present an article concerning optimising the LC-MS analysis of biomolecules.
Chromatographic Selectivity
Harnessing the Power of Chromatographic Selectivity
Capto Adhere Anion Exchanger for MAbs & Bind/Elute
Capto adhere from Cytiva is a multimodal strong anion exchanger for BioProcess applications.
How to Extend Liquid Chromatography Column Life: Regenerate or Use Guard Columns
What is causing aging of a column? Is the regeneration/washing of the column a reasonable approach to extend the lifetime of a column? What are the risks & challenges? Learn more in this article.
HPLC Analysis of Active Cannabinoid Compounds in Gummy Bear Candy on Ascentis® Express Biphenyl with UV Detection after Extraction
Tetrahydrocannabinolic acid A solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material.
Ascentis® Express F5 (Pentafluorophenyl) U/HPLC Columns
Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds
HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 15:85)
Separation of Sucrose; Maltose; Maltotriose
Chiral and Achiral LC-MS Analysis of Warfarin™ in Plasma Samples
Clinical laboratories are beginning to reap the benefits of LC-MS in terms of its sensitivity, specificity, throughput, and potential to reduce cost per sample. The purpose of this study was to demonstrate the LC-MS analysis of Warfarin in plasma samples
Protein G and Protein A Bind to Different IgG
This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.
HybridSPE-Phospholipid Technology
This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.
Ascentis® Express RP-Amide Columns for HPLC and UHPLC
Improve your separation of basic compounds with our Ascentis® Express RP-Amide HPLC columns from the Supelco® portfolio.
Determination of Ethyl Glucuronide and Ethyl Sulfate in Urine using LC-MS/MS
This study is aimed at developing and validating an LC-ESI-ion trap-MS/MS method for identification and quantification of ethyl glucuronide (EtG) and ethyl sulfate (EtS) as ethanol biomarkers for urine analysis.
HPLC Products: The Best Choice for Any LC Instrument
Discover our comprehensive portfolio of High-performance liquid chromatography (HPLC) columns and consumables for your various and specialized U/HPLC based applications― 100% compatible with HPLC, UHPLC, or UPLC® systems from any vendor.
Ion Chromatography & Cation Suppression Straightforward Cation Analysis at Trace Levels
Ammonium determination in acidic solutions with Metrosep C Supp 2 column using the acidic binary IC eluent nitric acid/rubidium nitrate concentrate and the sodium bicarbonate/sodium carbonate suppressor regenerant.
Chromatography Profile Analysis of Phosphorothioate Oligonucleotides
The ultimate goal of most antisense research is to develop therapeutic agents for diseases such as diabetes, cancer, and HIV/ AIDS. Phosphorothioate oligonucleotides (commonly referred to as S-oligos) are often the molecules of choice because of their resistance to cellular
Analysis of Oligonucleotides by SEC-MALS
Separation and identification of oligonucleotides differing by one base in length using Size Exclusion Chromatography combined with multi-angle light scattering (SEC-MALS) detector.
The Importance of Filtration in Optimizing (U)HPLC and LC-MS Performance
Learn how filter selection and sample and mobile phase filtration impact HPLC back pressure and column lifetime.
Quantitative Analysis of Nitrosamine Impurities by LC-MS Methods from the United States Pharmacopeia General Chapter <1469>
This application note describes the LC-MS-based quantitative analysis of known nitrosamine impurities following procedures 1 and 3 as given in USP general chapter .
Universal Hydrophilic Syringe Filters: Millex® Hydrophilic PTFE
Millex® hydrophilic PTFE syringe filters boast broad chemical compatibility and are HPLC-certified, guaranteeing low extractables. With their robust performance, these filters can be used for a variety of samples without the risk of membrane failure or sample contamination.
Determination of Ephedrine HCl and Pseudoephedrine HCl in Xiao’er Kechuanling Oral Solution using a Discovery® HS C18 Column
Developing an HPLC method for quantifying ephedrine HCl and pseudoephedrine HCl in traditional Chinese medicine (TCM) with a Discovery® C18 column to report: chromatographic data, linearity, specificity and repeatability, LOD and LOQ.
Ascentis ® Express HILIC U/HPLC Columns
For separation of polar compounds including polar neutrals, polar acids, and polar and non-polar basic amines use our Ascentis® Express HILIC column.
Three Methods for Critical Quality Attribute Determination of Monoclonal Antibodies
Step-by-step workflows for the intact mass analysis, peptide mapping, and N-glycan analysis of the monoclonal antibody― adalimumab, for an accurate characterization of the critical quality attributes (CQAs) to ensure drug safety and efficacy. Read more.
Fast and high-resolution Analysis of Intact Therapeutic Monoclonal Antibody Trastuzumab using BIOshell™ A400 Protein C4 Column
Optimization of a Reversed-Phase Liquid Chromatographic (RP-LC) method for the intact analysis of a therapeutic monoclonal antibody, trastuzumab, using a BIOshell™ A400 Protein C4 column.
Determination of Ephedrine HCl and Pseudoephedrine HCl in Xiao’er Kechuanling Oral Solution with Purospher® STAR RP-18e column
HPLC method to analyze ephedrine HCl and pseudoephedrine HCl in traditional Chinese medicine (TCM) with a Purospher® RP-18e colμmn to report chromatographic data, linearity, specificity and repeatability and LOD and LOQ.
Synthesis and HILIC/MS Analysis of Acylcarnitines
HILIC mode chromatography using an Ascentis® Express OH5 resolved the majority of the acyl-L-carnitines tested. It may be possible to use MS/MS and particular fragment ions to resolve the two coeluting pairs.
Extractables Studies of Single-Use Equipment Immediate Identification and Quantification of Unknown Extractables by GC/MS with a Certified Reference Material Mix for Extractables and Leachables
Identification and quantification of the most common extractables originating from single-use equipment by GC/MS and an Extractables and Leachables Screening Standard.
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