CN
Low pressure liquid chromatography
Applied Filters:
facet applications:Low pressure liquid chromatography
facet content type:Protocol
Showing 1-30 of 45 results
Selectivity and the Properties of a HIC Medium
Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.
Characteristics of Glutathione Sepharose® Products
Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.
Affinity Chromatography in a Purification Strategy (CIPP)
This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.
MonoBeads: Purification of Milligram Quantities with Highest Resolution
This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.
Chromatofocusing: Principles and Methods
This page covers the principles and methods of chromatofocusing, a chromatography technique that separates proteins according to differences in their isoelectric point (pI).
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
Purification using StrepTactin Sepharose® High Performance
This page shows how to purify Strep-tag II recombinant proteins using StrepTactin Sepharose® High Performance from Cytiva.
Sample Preparation in Ion Exchange Chromatography
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
Coupling Small Ligands Through Carboxyl Groups via a Spacer Arm
Ligands are coupled in a simple one-step procedure in the presence of a coupling reagent, carbodiimide. The carbodiimides may be regarded as anhydrides of urea.
Coupling Protocol for Primary Amine of a Ligand
This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.
Purification Using GSTrap™ HP, GSTrap™ FF, and GSTrap™ 4B Columns
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap columns packed with Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, or Glutathione Sepharose® 4B chromatography media.
Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
Sepharose High Performance: Purification with High Resolution
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up
This page covers the use of Sepharose Fast Flow for purification of proteins.
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose 6 Fast Flow
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from Cytiva. Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized and
Packing a Column and Performing a Separation with Sephadex® LH-20
This page shows how to perform a separation with a Sephadex LH-20 column from Cytiva which are Size Exclusion Chromatography media of polysaccharide network made from cross-linked hydroxypropylated dextran beads.
Performing a Separation with Superose
Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.
Removal of Detergents from Biological Product Matrices
The Amberlite™ XAD-4 resin used in Porozorb™ cartridges is a proven technology that is highly effective in removing various detergents from cell culture media for biopharmaceutical applications such as vaccine production.
Performing a Purification of Antibody Fragments with Capto™ L Products
This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.
Removal of Albumin Using Blue Sepharose® Chromatography Media
Remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.
Capto Core 700
Capto Core 700 from Cytiva is designed for intermediate purification and polishing of viruses and other large biomolecules in flowthrough mode.
Refolding of proteins using gel filtration
This page shows how to refold proteins using gel filtration and products from Cytiva.
Refolding using ion exchange chromatography and analysis of refolding
This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from Cytiva and how to analyze the refolding process with Superdex columns from Cytiva.
Purification of Histidine-Tagged Proteins using TALON® Superflow
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
Buffer Exchange and Desalting for Affinity Chromatography
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
Purification using StrepTrap™ HP 1 mL and 5 mL
This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.
Purification of Histidine-Tagged Proteins using HiTrap® IMAC HP and HiTrap® IMAC FF
This page shows how to purify histidine-tagged proteins using HiTrap® IMAC HP and HiTrap® IMAC FF from Cytiva.
Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance
Protocol for Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance
Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
Page 1 of 2