Skip to Content

CN

Expanded multilocus sequence typing for burkholderia species.

Expanded multilocus sequence typing for burkholderia species.

Journal of clinical microbiology (2009-06-06)

Theodore Spilker, Adam Baldwin, Amy Bumford, Chris G Dowson, Eshwar Mahenthiralingam, John J LiPuma

ABSTRACT

PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Betaine, BioUltra, ≥99.0% (NT)

Sigma-Aldrich

Betaine, ≥98% (perchloric acid titration)

Sigma-Aldrich

Dimethyl sulfoxide, PCR Reagent

Sigma-Aldrich

Dimethyl sulfoxide, anhydrous, ≥99.9%

Sigma-Aldrich

Dimethyl sulfoxide, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Sigma-Aldrich

Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications

Supelco

Dimethyl sulfoxide, analytical standard

Sigma-Aldrich

Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC)

Sigma-Aldrich

Dimethyl sulfoxide, ACS reagent, ≥99.9%

Sigma-Aldrich

Dimethyl sulfoxide, BioUltra, Molecular Biology, ≥99.5% (GC)

Sigma-Aldrich

Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture

Sigma-Aldrich

Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

Sigma-Aldrich

Dimethyl sulfoxide, Molecular Biology

Sigma-Aldrich

Dimethyl sulfoxide, suitable for HPLC, ≥99.7%

Sigma-Aldrich

Dimethyl sulfoxide, ReagentPlus^®, ≥99.5%

Sigma-Aldrich

Dimethyl sulfoxide, puriss. p.a., dried, ≤0.02% water

Sigma-Aldrich

Dimethyl sulfoxide, ≥99.6%, ReagentPlus^®