[Potentiometric glucose determination in human serum samples with glucose oxidase biosensor based on iodide electrode].

[Potentiometric glucose determination in human serum samples with glucose oxidase biosensor based on iodide electrode].

Prikladnaia biokhimiia i mikrobiologiia (2013-06-26)

E Karakus, Sh Pekyardimchi, E Kilich

ABSTRACT

Glucose potentiometric biosensor was prepared by immobilizing glucose oxidase on iodide-selective electrode. The hydrogen peroxide formed after the oxidation of glucose catalysed by glucose oxidase (GOD) was oxidized by sodium molybdate (SMo) at iodide electrode in the presence ofdichlorometane. The glucose concentration was calculated from the decrease of iodide concentration determined by iodide-selective sensor. The sensitivity of glucose biosensor towards iodide ions and glucose was in the concentration ranges of 1.0 x 10(-1) - 1.0 x 10(-6) M and 1.0 x 10(-2) - 1.0 x 10(-4) M, respectively. The characterization of proposed glucose biosensor and glucose assay in human serum were also investigated.

MATERIALS

Product Number

Brand

Product Description

357227

Sigma-Aldrich

Molybdenum, foil, thickness 0.025 mm, ≥99.9% trace metals basis

510092

Sigma-Aldrich

Molybdenum, powder, 1-5 μm, ≥99.9% trace metals basis

357200

Sigma-Aldrich

Molybdenum, foil, thickness 1.0 mm, ≥99.9% trace metals basis

266922

Sigma-Aldrich

Molybdenum, foil, thickness 0.1 mm, ≥99.9% trace metals basis

203823

Sigma-Aldrich

Molybdenum, powder, <150 μm, 99.99% trace metals basis

266892

Sigma-Aldrich

Molybdenum, powder, <150 μm, 99.9% trace metals basis

18304

Sigma-Aldrich

Hydrogen peroxide solution, 34.5-36.5%

480967

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Sodium molybdate dihydrate, 99.99% trace metals basis

M1651

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Sodium molybdate dihydrate, ≥99.5%, suitable for plant cell culture

M1003

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Sodium molybdate dihydrate, ≥99.5%

331058

Sigma-Aldrich

Sodium molybdate dihydrate, ACS reagent, ≥99%

243655

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Sodium molybdate, ≥98%

366986

Sigma-Aldrich

Molybdenum, powder, 10 μm, ≥99.95% trace metals basis

737860

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Sodium molybdate, powder, −100 mesh particle size, 99.9% trace metals basis

577987

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Molybdenum, nanopowder, <100 nm particle size (TEM), 99.8% trace metals basis

323381

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Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H₂O

88597

Millipore

Hydrogen peroxide solution, 3%, suitable for microbiology

349887

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Hydrogen peroxide solution, contains inhibitor, 35 wt. % in H₂O

266914

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Molybdenum, wire, diam. 1.0 mm, 99.95% trace metals basis

02575

Supelco

Dichloromethane, analytical standard

650463

Sigma-Aldrich

Dichloromethane, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 50-150 ppm amylene as stabilizer

676853

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320269

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Dichloromethane, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer

D65100

Sigma-Aldrich

Dichloromethane, contains 40-150 ppm amylene as stabilizer, ACS reagent, ≥99.5%

270997

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66742

Supelco

Dichloromethane, Selectophore^™, ≥99.5%

34856

Sigma-Aldrich

Dichloromethane, suitable for HPLC, ≥99.8%, contains amylene as stabilizer

439223

Sigma-Aldrich

Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer

24233

Sigma-Aldrich

Dichloromethane, puriss., meets analytical specification of Ph. Eur., NF, ≥99% (GC)

32222

Sigma-Aldrich

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