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  • Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts.

Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts.

Food chemistry (2014-05-20)
O Kenny, T J Smyth, D Walsh, C T Kelleher, C M Hewage, N P Brunton
ABSTRACT

Antimicrobial properties of ethanol and water extracts from eight Asteraceae species were investigated against three Gram positive (Staphylococcus aureus, MRSA and Bacillus cereus) and two Gram negative (Escherichia coli and Salmonella typhimurium) bacterial strains. Ethanol extracts from Centaurea scabiosa, Arctium minus, Taraxacum officinale, Centaurea nigra and Cirsium palustre demonstrated antimicrobial activity against strains of S. aureus, MRSA and B. cereus (MIC=187.5-365μg/ml). Ethanol extracts also had higher antioxidant activities and phenolic content demonstrating a link between these compounds and the bioactivity of these extracts. Further investigation into the phenolic content of ethanol extracts using UPLC-MS/MS lead to the identification and quantification of numerous phenolic compounds in all species including; 18 from Cirsium arvense, 16 from Cirsium vulgare, 19 from C. palustre, 15 from C. nigra, 17 from C. scabiosa, 14 from Sonchus asper, 17 from A. minus and 11 from T. officinale.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Vanillic acid, ≥97%, FG
Sigma-Aldrich
Methanol, suitable for NMR (reference standard)
Supelco
Kaempferol, analytical standard
Rosmarinic acid, primary reference standard
Sigma-Aldrich
L-Ascorbic acid, FCC, FG
Supelco
(−)-Epicatechin, analytical standard
Chlorogenic acid, primary reference standard
Supelco
Ethanol solution, certified reference material, 2000 μg/mL in methanol
Supelco
L-Ascorbic acid, analytical standard
Supelco
Vanillic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Rosmarinic acid, 96%
Supelco
Sodium carbonate, reference material for titrimetry, certified by BAM, >99.5%
Sigma-Aldrich
Sodium carbonate, BioUltra, anhydrous, ≥99.5% (calc. on dry substance, T)
Supelco
Sinapic acid, suitable for matrix substance for MALDI-MS, ≥99.0% (T)
Sigma-Aldrich
D-(−)-Quinic acid, 98%
Sigma-Aldrich
L-Ascorbic acid, 99%
Sigma-Aldrich
Kaempferol, ≥97.0% (HPLC)
Sigma-Aldrich
Ethyl gallate, antioxidant, ≥96.0% (HPLC)
Supelco
2,4,6-Tris(2-pyridyl)-s-triazine, for spectrophotometric det. of Fe, ≥99.0% (HPLC)
Sigma-Aldrich
Vanillic acid, purum, ≥97.0% (HPLC)
Millipore
2,3,5-Triphenyl-tetrazolium chloride solution, suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC
Sigma-Aldrich
Sodium carbonate, anhydrous, powder, 99.999% trace metals basis
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Supelco
Methanol, analytical standard
Sigma-Aldrich
L-Ascorbic acid, puriss. p.a., ACS reagent, reag. ISO, Ph. Eur., 99.7-100.5% (oxidimetric)
Sigma-Aldrich
L-Ascorbic acid, tested according to Ph. Eur.
Sigma-Aldrich
Capsaicin, from Capsicum sp., ≥50% (HPLC)
Sigma-Aldrich
L-Ascorbic acid, BioUltra, ≥99.5% (RT)
Sigma-Aldrich
Apigenin, ≥95.0% (HPLC)
Sigma-Aldrich
(−)-Epicatechin, ≥90% (HPLC)