5 g peanut butter in 40 mL vial
divinylbenzene-Carboxen-polydimethylbenzene StableFlex (57328-U)
headspace, 30 min at 65 °C in heating block
270 °C for 5 min
SUPELCOWAX 10, 30 m × 0.25 mm, 0.25 μm (24079)
40 °C (5 min) to 230 °C at 4 °C/min
270 °C
splitless/split, closed 0.5 min, 270 °C, with 0.75 mm liner
ion trap mass spectrometer, m/z =30-350 at 0.6 sec/scan. Selected ions used for quantitation.
Helium, 30 cm/sec
0.75 mm
peanut butter
In this application, SPME was used for the analysis of volatiles in multifloral honey using a headspace method. Headspace SPME (HS-SPME) using a Carboxen/PDMS/DVB fiber coupled with gas chromatography-mass spectrometry (GC/MS) was used for the characterization of the volatile fraction of honey samples. Using SPME it was possible to obtain and distinguish the chemical fingerprints of different types of honey. The relatively polar SUPELCOWAX 10 GC column provides excellent separation of these polar compounds.
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