2 g of coffee beans
50/30 μm divinylbenzene/Carboxen on polydimethylsiloxane on a StableFlex fiber (57329-U)
headspace, with a pre-equilibration period of 10 min. at 60 °C, and then a fiber exposure of 40 min. at 60 °C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 rpm
260 °C for 5 min
Omegawax 250, 30 m x 0.25 mm I.D., 0.25 μm (24136)
40 °C (5 min), 4 °C/min to 230 °C, 50 °C/min to 280 °C (2 min)
260 °C
MS, m/z 40-400
helium, 34.8 cm/sec constant
splitless
m/z 40-400
Roasted coffee
0.75 mm I.D. SPME/Splitless (2633901)
Headspace-SPME followed by GC analysis was used in this application to analyze volatile compounds in roasted coffee. SPME is ideal for analyzing the headspace of solid samples like coffee beans. The Carboxen/DVB/PDMS fiber is the fiber of choice for analyzing the wide range of analytes commonly found in roast coffee beans.The relatively polar Omegawax 250 GC column provided high resolution.
Omegawax is a trademark of Sigma-Aldrich Co. LLC