HPLC Analysis of Arbutin and Hydroquinone in Whitening Serum with Ascentis® Express C18
Materials
related product
used together
CONDITIONS
column
Ascentis® Express C18, 2.7 µm 100 x 2.1 mm I.D. (53832-U)
mobile phase
[A] Water; [B] Methanol; A/B 10/90 (v/v)
gradient
isocratic
flow rate
100 µL/min
pressure
81 bar (1175 psi)
column temp.
25 °C
detector
UV, 220 nm (micro flow cell; 1.4 µL/7 mm)
injection
0.5 µL
sample/matrix
Standard solution (10 µg/mL): Weigh accurately 5 mg of each standard into a 50 mL volumetric flask and add 40 mL diluent. Sonicate for 5 min and fill up to mark with diluent. Pipette 5 mL of this solution to a 50 ml volumetric flask and fill up to volume with diluent.
Sample preparation: Place 1 g of homogenized serum in a 50 mL volumetric flask, add 30 mL of diluent and sonicate for 5 minutes. Fill up to volume with diluent. Transfer 3 mL of this suspension into a 25 mL volumetric flask and fill up to volume with diluent. Pass through 0.45 µm syringe filter before injection.
Description
General description
Bleaching or skin whitening serum is widely used to reduce melanin in the skin, and for such products analytical quality control is required. This report focuses on the testing of arbutin and the toxic by-product hydroquinone in formulated serum products, using LC-UV.
For the analysis of arbutin and hydroquinone in skin lightening cream, the goal of this work is to improve existing methods (e.g. Int. J. Appl. Sci. Eng., 2011.9.4) using shorter and smaller particle size columns instead of a 250 x 4.6mm RP-18e 5 µm column.
Analysis Note
Here an Ascentis® Express C18 100 x 2.1 mm, 2.7 mm is used. An LOD <5 µg/mL and LOQ <10 µg/mL for arbutin, and a LOD <0.5 µg/mL LOQ <1 µg/mL of hydroquinone was achieved using a UV detection.
Other Notes
APP_226
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany