HPLC Analysis of Glucosinolates on Chromolith® RP-18 endcapped
Materials
related product
CONDITIONS
column
Chromolith® HighResolution RP-18 endcapped 200-2 (2x 100-2 mm columns coupled with 1.51467)
mobile phase
[A] Water; [B] acetonitrile
gradient
At 0 min, 100%A at 95µL/min; in 6.7 min, to 95%A at 190 µL/min; in 10.0 min to 80%A at 363 µL/min; in 3.3 min to 20%A at 363 µL/min.
flow rate
95-363 µL (see gradient)
column temp.
Ambient
detector
UV, 229 nm (micro flow cell; 1.4 µL/7 mm)
injection
1 µL
pressure
55-190 bar (798-2755 psi) (A031)
Description
General description
Glucosinolates are plant secondary metabolites present in Brassicaceae family plants, including broccoli, cauliflower, horseradish, and cabbage. When plants are chewed, cut, or otherwise damaged, glucosinolates form mustard oils that give the plants their pungent flavor. These oils have a characteristic bitter flavor and can be used for crop protection or as an antibacterial in medicinal products.In this method for the determination of glucosinolates, a Chromolith® HighResolution RP-18 endcapped column was used with UV detection.
Other Notes
App_634
Legal Information
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany