HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4
Materials
related product
Product No.
Description
Pricing
L-Lactic Dehydrogenase from rabbit muscle
Type XI, lyophilized powder, 600-1,200 units/mg proteinTrypsin Inhibitor from Glycine max (soybean)
BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)used together
Product No.
Description
Pricing
BIOshell™ A400 Protein C4, 3.4 μm Guard Cartridge
3.4 μm particle size, L × I.D. 0.5 cm × 2.1 mm, pkg of 3 eaCONDITIONS
column
BIOshell A400 Protein C4, 10 cm X 2.1 mm, 3.4 μm particles (66825-U)
mobile phase
(A) 75:25, [0.1% TFA in water]:[0.1% TFA in acetonitrile] ; (B) 25:75, [0.1% TFA in water]:[0.1% TFA in acetonitrile]
gradient
25 to 65% B in 8 min
flow rate
0.3 mL/min
pressure
1430 psi (99 bar)
detector
UV, 215 nm
injection
1 μL
sample
protein mixture, g/L ea: lysozyme, 0.1; haptoglobulin, 0.3; conalbumin, 0.3; trypsin inhibitor, 0.3; glutamate dehydrogenase, 0.8; beta-galactosidase, 0.7; ovalbumin, 0.8; lactate dehydrogenase, 0.4
Description
Analysis Note
This application demonstrates the utility of the BIOshell A400 Protein C4 column for the reversed-phase chromatography of proteins from a variety of sources and of different molecular weights.
Legal Information
BIOshell is a trademark of Sigma-Aldrich Co. LLC