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HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18

HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18 application for HPLC

Materials

related product

Product No.
Description
Pricing

T9128

Trypsin inhibitor from Glycine max (soybean)

lyophilized powder

A2327

Adrenomedullin 52 human

≥90% (HPLC), powder

R6513

Ribonuclease A from bovine pancreas

Molecular Biology, ≥70 Kunitz units/mg protein, lyophilized

62971

Lysozyme from chicken egg white

powder (crystalline), 70000-140000 U/mg

S7571

Superoxide Dismutase from bovine erythrocytes

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

41235

Ovalbumin-Atto 488 conjugate

suitable for fluorescence

53307-U

Ascentis® Express Peptide ES-C18 (2.7 μm) HPLC Column

L × I.D. 15 cm × 2.1 mm, pk of 1 ea

C6749

Cytochrome c equine

recombinant, expressed in E. coli

C2624

Carbonic Anhydrase from bovine erythrocytes

≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder

CONDITIONS

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)

mobile phase

[A] 75:25, 0.1% TFA in water: 0.08% TFA in acetonitrile; [B] 25:75, 0.1% TFA in water: 0.08% TFA in acetonitrile

gradient

held at 0% B for 0.5 min; 0 to 100% B in 7 min

flow rate

0.3 mL/min

pressure

4743 psi (327 bar)

column temp.

35 °C

detector

UV, 220 nm

injection

1 μL

sample

0.2 - 0.6 g/L in mobile phase A

Description

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Chromatogram Search

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HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS