HPLC-FLD Analysis of Bisphenol A in Olive Oil using a 4.6 mm ID Chromolith® Column and Ethanol in Mobile Phase - Green HPLC
Materials
related product
used together
Milli-Q® IQ 7000 Ultrapure Water Purification System
output: type 1 water (18.2 MΩ·cm), the most advanced Milli-Q® ultrapure (Type 1) water purification system that is intelligent, intuitive, and green.CONDITIONS
column
Chromolith® HighResolution RP-18 endcapped 100 x 4.6 mm (1.52022)
mobile phase
Water/Ethanol 51/49 (v/v)
gradient
isocratic
flow rate
1 mL/min
pressure
69 bar (1001 psi)
column temp.
30 °C
detector
FLD; Ex: 275 nm; Em: 305 nm
sample/matrix
Olive Oil spiked at 25 µg/g with BPA - Weigh 2.0 g of olive oil in a 15 mL screw-cap test tube, add 1 mL of the spike solution (50 µg/mL in, seal with screw cap and shake for 30 seconds. Add 4 mL of diluent and shake for 1 minute. Sonicate the solution for 15 minutes at room temperature and after that centrifuge it at 5000 rev/min for 25 min. Take an aliquot of the supernatant and put it through a 0.45 µm PTFE Filter.
injection
5 µL (A037)
Description
General description
Bisphenol A (BPA) is a precursor to important plastics and can be found in many different consumer goods, e.g. plastic bottles. It is soluble in organic solvents and slightly soluble in water. Due to this characteristic leaching of BPA from plastic bottles into the bottle content can be observed. BPA is a xenoestrogen with the ability to mimic the human hormone estrogen.
Analysis Note
This application work is focused on combining HPLC with more sustainable solvents. Therefore, the analysis of BPA content in olive oil was developed by using ethanol as an organic modifier in the mobile phase and as extraction solvent.
For the HPLC separation, a matrix tolerant Chromolith® HighResolution RP-18 endcapped column 100 x 4.6 mm I.D. combined with fluorescence detection (FLD) was applied. A spiked olive oil sample (25 µg/g) was only extracted ethanol and filtered through a 0.45 µm PTFE Filter.
Other Notes
App_727
Legal Information
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany