HPLC-UV (254 nm) Analysis of Neonicotinoids on Chromolith® HR RP-18e Column with 2 mm I.D.
Materials
related product
CONDITIONS
column
Chromolith® HighResolution RP-18e 100 x 2 mm (1.52322)
mobile phase
[A] Water + 0.1% formic acid;
[B] Acetonitrile + 0.1% formic acid
gradient
(0 min 100% A; 0-3 min to 90% A; 3-5 min to 70% A, 5-12 min to 60% A; 12-12.4 min to 100% A; 12.4 min to 16 min 100% A)
flow rate
250 µL/min
pressure
58-69 bar (841-1001 psi)
column temp.
Ambient
detector
UV, 254 nm
injection
1 µL
sample/matrix
Neonicotinoid standard 100 µM each compound in acetonitrile
Description
General description
Neonicotinoids are water-soluble insecticides. They are used for crop protection and can migrate from treated soil into plant tissue and flower nectar. Neonicotinoids have a negative effect on the brain cells of honeybees, possibly leading to the collapse of colonies. [Clothianidin, Imidacloprid and Thiamethoxam are banned in Germany, (EU) No. 485/2013]
Analysis Note
Here an HPLC-UV separation of 7 neonicotinoids on a Chromolith® HR RP-18e column with 2 mm ID is shown, achieving baseline separation in under 9 minutes. The chomatogram shown is for UV detection at 254 nm. For Dinotefuran, Imidacloprid, and Clothianidin a detection at 270 nm provides higher sensitivities.
Other Notes
APP_267
Legal Information
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany