UHPLC Analysis of Nucleosides on Purospher® STAR RP-18 endcapped
Materials
standard
CONDITIONS
column
Hibar Purospher® STAR RP-18 endcapped, 150 x 4.6 mm, 3 μm (1.50470.0001)
mobile phase
[A] 30 mM ammonium phosphate, pH 5.3 with ammonium hydroxide in water; [B] Methanol; 97.5 [A] / 2.5 [B]
flow rate
1.0 mL/min
pressure
3060 psi 211 Bar
column temp.
25 °C
detector
UV 254
injection
5 μL
sample
5 μg/mL of 12 nucleosides in 98:2 30mM ammonium phosphate pH 5.3 with ammonium hydroxide in water: methanol
Description
Analysis Note
Nucleosides are structural subunits of nucleic acids, the macromolecules that convey genetic information in living cells. They contain a molecule of sugar (pentose) bonded to a nitrogen-containing organic ring compound. The nitrogen-containing compound is either a pyrimidine (cytosine, thymine, or uracil) or a purine (adenine or guanine). Nucleosides, derivatives and / or analogues can be used in many different industries and applications as in agrochemistry (herbicides, fungicides and insecticides), therapeutic drugs including a range of antiviral (HIV, hepatitis C virus and herpes simplex) and even antibiotics.
Legal Information
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany