LC-MS Analysis of Sterols with Ascentis® Express C18
Materials
related product
Aldosterone solution
100 μg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Corticosterone solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®17α-Methyltestosterone solution
1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant®Testosterone solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis® Express 90 Å C18, 2.7 µm, 10 cm x 2.1 mm, (53823-U)
mobile phase
[A]Water, 5 mM ammonium formate, 0.1% formic acid, pH 4.0; [B] Methanol
gradient
0-3 min 60% B, 3-5 min to 9% B, 5-7.5 min 95% B, 7.5-7.51 to 60% B, 7.51-11 min 60% B
flow rate
0.3 mL/min
pressure
190 bar
column temp.
50 °C
detector
MS/MS +ESI/ -ESI; spray voltage: 3.0 kV; nebulizing gas: 2 L/min; drying gas: 15 L/min; DL temp: 250 ˚C; heat block: 400 ˚C; MRM transitions see Analysis Notes
injection
2 µL
sample/matrix
Steroid standards of 10 ng/mL in 45% methanol and 55% water
Description
General description
Synthetic hormones and steroids have found application in the food industry, and many animals in the food chain have been exposed to high levels of steroids during their lifetime. Steroid hormones are naturally occurring, lipophilic compounds, that regulate many essential functions in the human body. Increased exposure to high levels of steroids, however, has been linked to cardiovascular system damage and cancer. Steroids, which are typically used as growth promoters and those used for therapeutic purposes, were screened in beef samples.
Analysis Note
MRM Transitions: Aldostrerone 361.0000>343.1000, Corticosterone 347.6000>109.0000, α-Zearalanol 321.0000>277.0000, Testosterone 289.0000>109.0000, 17α-Methyltestosterone 303.1000>97.0000, and Progesterone 315.0000>109.1000
Other Notes
APP_573
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany