Skip to Content
Merck
CN

UHPLC/MS Analysis of Cannabinoids on Ascentis® Express C18

UHPLC/MS Analysis of Cannabinoids on Ascentis® Express C18 application for UHPLC-MS

Materials

related product

Product No.
Description
Pricing

C-045

Cannabidiol solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-046

Cannabinol solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

T-005

(−)-trans-Δ9-THC solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

50813-U

Ascentis® Express C18, 2 μm UHPLC Column

2 μm particle size, L × I.D. 10 cm × 2.1 mm

C-140

Cannabidivarin solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-141

Cannabigerol solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-142

Cannabigerolic acid solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

C-143

Cannabichromene solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-144

Cannabidiolic acid solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 μm particles (50813-U)

mobile phase

[A] 0.1% formic acid; [B] 0.1% formic acid in acetonitrile

gradient

60% B to 100% B in 10 min

flow rate

0.4 mL/min

pressure

7200 psi (496 bar)

column temp.

35 °C

detector

MS, ESI(+), ESI(-), MRM m/z (see figure for transitions)

injection

1 μL

sample

100 ng/mL each in methanol

Description

Analysis Note

Cannabis compounds reportedly have therapeutic efficacy in the treatment of pain, mood disorders, and inflammatory diseases. These standards are used in testing methods by GC/MS, LC/MS, or HPLC for applications in clinical toxicology, testing of cannabis potency or impurity profiling by growers, pharmaceutical research, forensic analysis, and urine drug testing. Shown here is the separation of cannabis compounds on an Ascentis Express C18 column. Highest grade UHPLC solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS